Search results for "spore"
showing 10 items of 252 documents
Biological and microclimatic monitoring for conservation of cultural heritage: a case study at the De Rossi room of the Palatina library in Parma
2019
Biological particles in heritage-related indoor environments (museums, libraries, archives) represent a hazard to artifacts (biodeteriogenic action), operators and visitors’ health. The aim of the study was to evaluate environmental biological contamination and microclimate conditions in different periods of the year in De Rossi room of the historical Palatina Library in Parma. Microclimatic measurements were recorded continuously for a period ranging from 11 to 17 days in: January–February, May and September 2017. Monitoring of bacterial and fungal contamination was performed for air by active and passive sampling and by nitrocellulose membranes on shelves and manuscripts. Microorganisms w…
A new highly diverse palynoflora from the Lower Devonian Nogueras Formation of the Iberian Peninsula
2015
AbstractA new well-preserved spore assemblage has been discovered from the Lower Devonian Nogueras Formation of Mezquita de Loscos (Teruel Province, north-eastern Spain). The palynoflora includes 34 spore species belonging to 20 genera, among which 14 are new for the locality, e.g. Apiculiretusispora, Brochotriletes, Cirratriradites, Iberoespora, Knoxisporites and Verrucosisporites. The assemblage is mainly composed of trilete spores, while tripapillate and monolete forms are also observed. An early Pragian age is suggested for the fossil site. Specimens of Latosporites ovalis, a species previously well-documented only from the late Pragian–Emsian of Saudi Arabia and Brazil, are found in th…
Étude paléobotanique d’une lentille argileuse cénomanienne de la carrière de Hucheloup, Maine-et-Loire, NO France : implications taxonomiques, strati…
2017
International audience; A Cenomanian clay lens in Hucheloup Quarry (Maine-et-Loire, north-western France) has yielded 82 foliar compressions and impressions, of which 62 were identified at several taxonomic levels. The plants are ascribed to diverse Pinales (27%), ferns (27%), Ginkgoales (26%), angiosperms (19%) and Cycadales (1%). The most abundant species in the assemblage are Frenelopsis alata (K. Feistmantel) E. Knobloch emend. J. Kvaček (Pinales) and Eretmophyllum obtusum (Velenovský) J. Kvaček (syn. E. andegavense Pons, Bourreau et Broutin) (Ginkgoales). Wood fragments, along with very well-preserved cuticles, have also been recovered and identified. The specimens are usually very fra…
Gametic and somatic embryogenesis through in vitro anther culture of different Citrus genotypes
2015
Abstract: In vitro tissue culture represents a useful technique for advancing Citrus breeding and propagation. Among in vitro regeneration systems, anther culture is commonly used to produce haploids and doubled haploids for a fast-track producing homozygous lines, in comparison with the traditional self-pollination approach, which involves several generations of selfing. In addition, anthers culture can produce somatic embryos that can also be used for clonal propagation. In this study, two thermal shocks were applied to the anthers of six Citrus genotypes (two clementine and four sweet oranges), just after they were put in culture. The response obtained was different depending on the geno…
Give me a sample of air and I will tell which species are found from your region : Molecular identification of fungi from airborne spore samples
2017
Fungi are a megadiverse group of organisms, they play major roles in ecosystem functioning and are important for human health, food production and nature conservation. Our knowledge on fungal diversity and fungal ecology is however still very limited, in part because surveying and identifying fungi is time demanding and requires expert knowledge. We present a method that allows anyone to generate a list of fungal species likely to occur in a region of interest, with minimal effort and without requiring taxonomical expertise. The method consists of using a cyclone sampler to acquire fungal spores directly from the air to an Eppendorf tube, and applying DNA barcoding with probabilistic specie…
Microspore Embryogenesis Through Anther Culture in Citrus clementina Hort. ex Tan.
2016
Anther culture is a biotechnological method that allows to obtain, in one step, homozygous plants, very important to plant breeding, due to their numerous applications in mutation research, selection, genome sequencing, genetic analysis, and transformation. To induce the microspores, i.e., the immature male gametes, to switch from the normal gametophytic pathway to the sporophytic one, it is necessary to submit them to a type of stress, such as high or low temperature, starvation, or magnetic field. Stress can be applied to the donor plants and/or the fl oral buds or the anthers or the isolated microspores, before or during the culture. In this chapter, the protocol to induce gametic embryo…
Microspore embryogenesis induced through in vitro anther culture of almond (Prunus dulcis Mill.)
2016
Anther culture is one of the most widely used methods to induce gametic embryogenesis. The aim of this investigation was to induce microspore embryogenesis in almond (Prunus dulcis Mill.), through this technique. Anthers were cultured at the vacuolated developmental stage, and seven cultivars, two culture media and two temperature treatments were assessed. Although evidence of the microspore induction was observed in all the genotypes and treatments tested (symmetrical nucleus division and multinucleated structures), calli were produced merely by anthers cultured in the medium P and the regeneration of embryos was detected only in anthers of the cultivars Filippo Ceo, Lauranne and Genco, pl…
Single-Spore Extraction for Genetic Analyses of Arbuscular Mycorrhizal Fungi
2020
International audience; Biomass of arbuscular mycorrhizal fungi (AMF, Glomeromycota) is often only available in small quantities as these fungi are obligate biotrophs and many species are difficult to cultivate under controlled conditions. Here, I describe a simple, efficient approach to produce crude extracts from single or a small number of spores that can be used for genotyping AMF.
Two ectomycorrhizal truffles, Tuber melanosporum and T. aestivum , endophytically colonise roots of non‐ectomycorrhizal plants in natural environments
2020
International audience; Serendipitous findings and studies on Tuber species suggest that some ectomycorrhizal fungi, beyond their complex interaction with ectomycorrhizal hosts, also colonise roots of nonectomycorrhizal plants in a loose way called endophytism. Here, we investigate endophytism of T. melanosporum and T. aestivum . We visualised endophytic T. melanosporum hyphae by fluorescent in situ hybridisation on nonectomycorrhizal plants. For the two Tuber species, microsatellite genotyping investigated the endophytic presence of the individuals whose mating produced nearby ascocarps. We quantified the expression of four T. aestivum genes in roots of endophyted, non‐ectomycorrhizal plan…
Gametic embryogenesis through isolated microspore culture in Corylus avellana L.
2015
Haploid technology is a valuable plant breeding tool for obtaining homozygosity particularly in woody plants. Hazelnut, the world’s sixth ranking nut tree crop is a monoecious, anemophilous species. It is characterized by a sporophytic incompatibility system that prevents production of homozygous plants with conventional methods, involving several self-pollination cycles. In this study, gametic embryogenesis, in particular isolated microspore culture, was tried with five genotypes. Two culture media were tested and four temperature stress treatments were applied to the isolated microspores that were cultivated at the vacuolated developmental stage. To our knowledge, this is the first report…