Search results for "start"
showing 10 items of 361 documents
Cloning and sequencing of the dnaK region of Streptomyces coelicolor A3(2)
1993
Abstract The dnaK homologue of Streptomyces coelicolor A3(2) strain M145 has been cloned and sequenced. Nucleotide sequence analysis of a 2.5-kb region revealed an open reading frame (ORF) encoding a predicted DnaK protein of 618 amino acids (Mr = 66 274). The dnaK coding sequence displays extreme codon bias and shows a strong preference for CGY and GGY, for Arg and Gly codons, respectively. The predicted DnaK sequence has a high Lys:Arg ratio which is not typical of streptomycete proteins. The region immediately downstream from dnaK contains an ORF for a GrpE-like protein; the predicted start codon of grpE overlaps the last two codons of dnaK, indicating that the two genes are translationa…
Molecular analysis of the dominant lactic acid bacteria of chickpea liquid starters and doughs and propagation of chickpea sourdoughs with selected W…
2020
Abstract Fermented chickpea liquid is used as a leavening agent in chickpea bread production. In the present study, traditional chickpea liquid starter and dough samples were collected from bakeries in Turkey and microbiologically investigated. Culture-independent analysis for microbiota diversity, performed by MiSeq Illumina, identified Clostridium perfringens as major group in all samples, while Weissella spp. Dominated LAB community. A culture-dependent methodology was applied and 141 isolates were confirmed to be members of the LAB group based on 16s rRNA gene sequence analysis. In particular, 11 different LAB species were identified confirming the high frequency of isolation of weissel…
The spatial distribution of bacteria in Grana-cheese during ripening.
2012
The microbial composition and its spatial distribution of Grana Trentino, a hard Parmesan-like cheese, was determined, from vat milk to cheese. After cutting along the vertical axis of the cheese wheels, three layers were sampled diagonally across the cheese: under the cheese rind, an intermediate section and the cheese core. After two different ripening periods (9 and 18 months), the cheese samples were analysed using traditional culture dependent and culture independent methods. Milk samples were dominated by mesophilic and psychrophilic bacterial counts. Thermophilic bacteria (Lactobacillus helveticus) were found in high amounts in cooked whey and natural whey starter cultures. After 9 m…
Identification of Critical Genes for Growth in Olive Brine by Transposon Mutagenesis of Lactobacillus pentosus C11
2013
ABSTRACT Olive brine represents a stressful environment due to the high NaCl concentration, presence of phenolic compounds known as antimicrobials, and low availability of nutrients. Thus, only a few strains of lactic acid bacteria (LAB) are adapted to grow in and ferment table olives. To identify the mechanisms by which these few strains are able to grow in olive brine, Lactobacillus pentosus C11, a particularly resistant strain isolated from naturally fermented table olives, was mutagenized by random transposition using the P junc -TpaseIS 1223 system (H. Licandro-Seraut, S. Brinster, M. van de Guchte, H. Scornec, E. Maguin, P. Sansonetti, J. F. Cavin, and P. Serror, Appl. Environ. Microb…
Programmēšanas apguves tīmekļa vietnes moduļu izstrāde
2018
Start(IT) ir projekts, kura mērķis ir palielināt programmēšanas apguves iespējas Latvijas vispārizglītojošās skolās. Lai atvieglotu mācības, tika izveidota tīmekļa vietne - sistēma, kurā to ērti var izdarīt. Kvalifikācijas darba ietvaros tīmekļa vietnei, kas veidota Ruby programmēšanas valodā Ruby on Rails ietvarā, tiek izstrādāti jauni moduļi, kas ļaus sistēmas lietotājiem apgūt konkrētus kursus, sekot līdzi mācību progresam, pārbaudīt savas zināšanas testos, kā arī pievienotas jaunas iespējas lietotāju profilos. Sistēmas dati tiek uzglabāti PostgreSQL datubāzē, savukārt sistēma ir veidota, izmantojot jaukto metodi, iekļaujot tajā spējās (Agile) un ūdenskrituma (waterfall) izstrādes metode…
Selection of Debaryomyces hansenii isolates as starters in meat products based on phenotypic virulence factors, tolerance to abiotic stress condition…
2021
Debaryomyces hansenii is a yeast widely used in meat fermentations as starter for the purpose of improving the aromatic quality of the final product. However, it has not been the subject of an extensive study regarding phenotypic characteristics important for starter selection, such as the capacity to grow at abiotic stress conditions occurring during fermentation, the ability to generate desirable aromas and the absence of virulence traits in yeasts.
Validation of a New Method for the Diagnosis of Rotor bar Failures via Wavelet Transformation in Industrial Induction Machines
2006
[EN] In this paper, the authors propose a method for the diagnosis of rotor bar failures in induction machines, based on the analysis of the stator current during the startup using the discrete wavelet transform (DWT). Unlike other approaches, the study of the high-order wavelet signals resulting from the decomposition is the core of the proposed method. After an introduction of the physical and mathematical bases of the method, a description of the proposed approach is given; for this purpose, a numerical model of induction machine is used in such a way that the effects of a bar breakage can clearly be shown, avoiding the influence of other phenomena not related with the fault. Afterward, …
The Use of the Wavelet Approximation Signal as a Tool for the Diagnosis of Rotor Bar Failures
2005
[EN] The aim of this paper is to present a new approach for rotor bar failure diagnosis in induction machines. The method focuses on the study of an approximation signal resulting from the wavelet decomposition of the startup stator current. The presence of the left sideband harmonic is used as evidence of the rotor failure in most diagnosis methods based on the analysis of the stator current. Thus, a detailed description of the evolution of the left sideband harmonic during the startup transient is given in this paper; for this purpose, a method for calculating the evolution of the left sideband during the startup is developed, and its results are physically explained. This paper also show…
A quantitative-based model to assess seed accelerators’ performance
2021
Seed accelerators are a new generation of business incubators. While the number of seed accelerators worldwide has grown exponentially, there is as yet no consensus on how to measure and analyse th...
Knowledge assets for internationalization strategy proposal
2021
[EN] Lack of trust, lack of references and the confidential nature of cybersecurity projects make internationalization in companies from the cybersecurity sector a great challenge. The development of lean methodologies over recent years has presented a method to reduce time and effort, measure performance in each step and pivot when it is necessary to apply a process to a different field. Considering that internationalization resembles entrepreneurship, because of uncertainty and risk in a new market where the value proposition should be validated, the Lean Start-up philosophy will serve as a theoretical framework in which to operate. The study of international challenges of cybersecurity c…