Search results for "subtil"

Antagonistic activity of Bacillus subtilis strain AG1 against fungal microorganisms associated with Vitis vinifera.

2009

Wykorzystanie antagonistycznych właściwości Bacillus subtilis wobec Rhizoctonia solani

2017

Preorganization and reorganization as related factors in enzyme catalysis: the chorismate mutase case.

2003

In this paper a deeper insight into the chorismate-to prephenate-rearrangement, catalyzed by Bacillus subtilis chorismate mutase, is provided by means of a combination of statistical quantum mechanics/molecular mechanics simulation methods and hybrid potential energy surface exploration techniques. The main aim of this work is to present an estimation of the preorganization and reorganization terms of the enzyme catalytic rate enhancement. To analyze the first of these, we have studied different conformational equilibria of chorismate in aqueous solution and in the enzyme active site. Our conclusion is that chorismate mutase preferentially binds the reactive conformer of the substrate--that…

Biosorption of lead(II), zinc(II) and nickel(II) from industrial wastewater by Stenotrophomonas maltophilia and Bacillus subtilis

2015

Abstract The biosorption of Pb(II), Zn(II) and Ni(II) from industrial wastewater using Stenotrophomonas maltophilia and Bacillus subtilis was investigated under various experimental conditions regarding pH, metal concentration and contact time. The optimum pH values for the biosorption of the three metals were in the range 5.0-6.0, while the optimal contact time for the two bacterial species was 30 min. Experimental data was analyzed using Langmuir and Freundlich isotherms; the former had a better fit for the biosorption of Pb(II), Zn(II) and Ni(II). The maximum adsorption uptakes (qmax) of the three metals calculated from the Langmuir biosorption equation for S. maltophilia were 133.3, 47.…

Induction of cryptic metabolites of the endophytic fungus Trichocladium sp. through OSMAC and co-cultivation

2019

The endophytic fungus Trichocladium sp. isolated from roots of Houttuynia cordata was cultured on solid rice medium, yielding a new amidepsine derivative (1) and a new reduced spiro azaphilone derivative (3) together with eight known compounds (4–11). Co-cultivation of Trichocladium sp. with Bacillus subtilis resulted in induction of a further new compound (2) and a 10-fold increase of 11 compared to the axenic fungal culture. Moreover, when the fungus was cultivated on peas instead of rice, a new sesquiterpene derivative (13) and two known compounds (12 and 14) were obtained. Addition of 2% tryptophan to rice medium led to the isolation of a new bismacrolactone (15). The structures of the …

Chemoselektive Fluoreszenzstudien am Subtilisin und “Thiosubtilisin”

1985

Chemoselective Fluorescence Studies with Subtilisin and “Thiosubtilisin” The serine hydroxy group in the active site of subtilisin is selectively “phosphylated”3). This is demonstrated using the fluorescent phosphinic fluoride 1. “Thiosubtilisin” does not react with 1 but with the SH-selective and fluorescent vinyl sulfone 2.

ERA-experiment “space biochemistry”

1995

Abstract The general goal of the experiment was to study the response of anhydrobiotic (metabolically dormant) microorganisms (spores of Bacillus subtilis, cells of Deinococcus radiodurans, conidia of Aspergillus species) and cellular constituents (plasmid DNA, proteins, purple membranes, amino acids, urea) to the extremely dehydrating conditions of open space, in some cases in combination with irradiation by solar UV-light. Methods of investigation included viability tests, analysis of DNA damages (strand breaks, DNA-protein cross-links) and analysis of chemical effects by spectroscopic, electrophoretic and chromatographic methods. The decrease in viability of the microorganisms was as exp…

UJI AKTIVITAS ANTIBAKTERI DARI ESTRAK TUMBUHAN AKWAY (Drimys beccariana. Gibbs)

2010

<p><em>The plant Akway (Drimys beccariana.</em>Gibbs<em>) is endemic to Papua and is frequently used traditionally by the Arfak tribe as a medicinal plant. We extract the plant by maceration with ethanol. The antibacterial test  is done by Agar diffusion method. As microba test  we used Escheresia coli and Bacillus subtilis. Result obtained from fitochemistry skrinning of the leaf and bark contained alkaloid, flavonoid, steroid, tannin, glikosida, saponin, triterpenoid, steroid and  fenolik. Concentration of active substances from the leaf are flavonoid (0,3680%) and tanin (10.33 %); while from the bark flavonoid 18.35%  and tanin (27.65%).  Antibacterial test showed…

Succinate dehydrogenase functioning by a reverse redox loop mechanism and fumarate reductase in sulphate-reducing bacteria.

2006

Sulphate- or sulphur-reducing bacteria with known or draft genome sequences (Desulfovibrio vulgaris, Desulfovibrio desulfuricans G20, Desulfobacterium autotrophicum [draft], Desulfotalea psychrophila and Geobacter sulfurreducens) all contain sdhCAB or frdCAB gene clusters encoding succinate : quinone oxidoreductases. frdD or sdhD genes are missing. The presence and function of succinate dehydrogenase versus fumarate reductase was studied. Desulfovibrio desulfuricans (strain Essex 6) grew by fumarate respiration or by fumarate disproportionation, and contained fumarate reductase activity. Desulfovibrio vulgaris lacked fumarate respiration and contained succinate dehydrogenase activity. Succi…

Immunohistochemical localization of the pro-peptide processing enzymes PC1/PC3 and PC2 in the human anal canal.

1997

Abstract HORsch, D., R. Day, N. G. Seidah, E. Weihe and M. K.-H. SchAFer. Immunohistochemical localization of the pro-peptide processing enzymes PC1/PC3 and PC2 in the human anal canal. Peptides 18(5) 755–760, 1997.—The distribution of prohormone/pro-peptide convertases PC1/PC3 and PC2 was investigated in the human anal canal by immunohistochemistry. Both prohormone convertases exhibited region-specific distribution patterns and were observed in neural and neuroendocrine cells and in nonneuroendocrine cellular elements. PC1/PC3 immunoreactivity was present in enteric neurons, subsets of nerve fibers, and neuroendocrine cells, and also in epithelial cells like intestinal stem cells, and a su…