Search results for "techniques"

Protocol for Specific Isolation of Virulent Strains of Vibrio vulnificus Serovar E (Biotype 2) from Environmental Samples

2004

ABSTRACT The eel pathogen Vibrio vulnificus biotype 2 comprises at least three serovars, with serovar E being the only one involved in both epizootics of eel vibriosis and sporadic cases of human infections. The virulent strains of this serovar (VSE) have only been recovered from clinical (mainly eel tissue) sources. The main objective of this work was to design and validate a new protocol for VSE-specific isolation from environmental samples. The key element of the new protocol is the broth used for the first step (saline eel serum broth [SEB]), which contains eel serum as a nutritive and selective component. This approach takes advantage of the ability of VSE cells to grow in eel serum an…

Molecular Typing Reveals Frequent Clustering among Human Isolates of Listeria monocytogenes in Italy

2009

In Italy, the annual incidence of reported cases of listeriosis amounts in recent years (2004 to 2006) to 0.8 cases per million inhabitants. Our study is a subtyping analysis by serotyping, ribotyping, and pulsed-field gel electrophoresis analysis of 44 human isolates from apparently sporadic cases of infection in the Lombardy region and in the Province of Florence, Italy, in the years 1996 to 2007. Based on the results of the different subtyping methods, 10 occasions were detected when strains of L. monocytogenes with the same subtype were isolated from more than one listeriosis case. A total of 28 (66.7%) out of 44 isolates were attributed to molecular subtype clusters. Our data support t…

Il settore dell’olivicoltura da tavola in Italia: Situazione e prospettive

2020

Table olive Industry in Italy The Italian table olive industry is almost completely located in the southern regions: Sicily and Apulia together account for about two thirds of the entire national production. “Nocellara del Belice”, “Nocellara etnea”, “Bella di Cerignola”, “Ascolana” and “Itrana” are the leading cultivars, together with a large number of other local traditional varieties, often utilised for a double purpose (table olives and oil production). Although Italy is nowadays largely dependent (more than 50%) on importations from other countries (mainly Spain, Greece and Morocco) the domestic production has been declining in the last thirty years and currently accounts for about 2-3…

Advances in cultivation of almonds: effects of genotypes, environment and cultural techniques

2019

Advances in cultivation of almonds: effects of genotypes, environment and cultural techniques

Analysis of microbial colonization

2012

CONTENT OF THE BOOK The book takes into consideration archaeological artefacts made out of the most common materials, like stones (both natural and artificial), mosaics, ceramics, glass, metals, wood and textiles. But all these important materials are accompanied by less diffuse artefacts and materials, like clay tablets, goldsmith artefacts, icons, leather and skin objects, bones and ivory, coral and mother of pearl. Each type of artefact and/or material is treated from at least four different points of view: - Composition and processing technology - Alteration and degradation causes and mechanisms - Procedures for conservative intervention - Case study and/or examples of conservative inte…

Impressions from the 4th EDGG Research Expedition to Sicily: community composition and diversity of Mediterranean grasslands.

2012

Sexual dichromatism in Mediterranean storm petrels Hydrobates pelagicus melitensis.

2010

We propose the use of a non-invasive technique for sexing the Mediterranean subspecies of the European storm petrel Hydrobates pelagicus melitensis. We found that this subspecies shows sexual dimorphism in rump band width and wing length. Fast and cheap sex identification methods can improve conservation programs and population studies of this species.

Wharton’s Jelly Mesenchymal Stromal Cells Support the Expansion of Cord Blood–derived CD34+Cells Mimicking a Hematopoietic Niche in a Direct Cell–cel…

2018

Wharton’s jelly mesenchymal stromal cells (WJ-MSCs) have been recently exploited as a feeder layer in coculture systems to expand umbilical cord blood–hematopoietic stem/progenitor cells (UCB-HSPCs). Here, we investigated the role of WJ-MSCs in supporting ex vivo UCB-HSPC expansion either when cultured in direct contact (DC) with WJ-MSCs or separated by a transwell system or in the presence of WJ-MSC–conditioned medium. We found, in short-term culture, a greater degree of expansion of UCB-CD34+cells in a DC system (15.7 ± 4.1-fold increase) with respect to the other conditions. Moreover, in DC, we evidenced two different CD34+cell populations (one floating and one adherent to WJ-MSCs) with …

Metilazione del DNA in artrite reumatoide

2005

Lo stato di metilazione del DNA genomico e del gene PTHrP è stato valutato con tecniche molecolari e citogenetiche in artrite reumatoide (AR), patologia autoimmune caratterizzata anche da alta incidenza di linfomi e da ipercalcemia per overespressione del gene PTHrP. La metilazione del DNA, infatti, ha un ruolo critico nello sviluppo delle malattie neoplastiche; il gene PTHrP avendo tre promotori uno dei quali contiene un’isola CpG è un buon candidato per la deregolazione da alterato pattern di metilazione locale. Le indagini sulla metilazione genomica, condotte su DNA estratto da sangue periferico di pazienti e di donatori e amplificato in reazioni di Methylation-Sensitive Arbitrarily Prim…

Accretion in young stars: measure of the stream velocity of TW Hya from the X-ray Doppler shift

2015

High-resolution X-ray spectra are a unique tool to investigate the accretion process in young stars. In fact X-rays allow to investigate the accretion-shock region, where the infalling material is heated by strong shocks due to the impact with the denser stellar atmosphere. Here we show for the first time that it is possible to constrain the velocity of the accretion stream by measuring the Doppler shift of the emitted X-rays. To this aim we analyzed the deep Chandra/HETGS observation of the accreting young star TW Hya. We selected a sample of emission lines free from significant blends, fitted them with gaussian profiles, computed the radial velocity corresponding to each line, and average…