Search results for "testing"

Validación y verificación analítica de los métodos microbiológicos

2015

Clinical microbiologists should ensure, to the maximum level allowed by the scientific and technical development, the reliability of the results. This implies that, in addition to meeting the technical criteria to ensure their validity, they must be performed with a number of conditions that allows comparable results to be obtained, regardless of the laboratory that performs the test. In this sense, the use of recognized and accepted reference methodsis the most effective tool for these guarantees. The activities related to verification and validation of analytical methods has become very important, as there is continuous development, as well as updating techniques and increasingly complex …

Comparison between Disk Diffusion and Microdilution Methods for Determining Susceptibility of Clinical Fungal Isolates to Caspofungin

2007

ABSTRACT We compared the caspofungin (CAS) susceptibility testing results generated by the disk diffusion (DD) assay with the results of the Clinical and Laboratory Standards Institute (CLSI) broth microdilution (BD) reference method for 106 yeast isolates. The isolates represented 11 different fungal species, including Candida albicans ( n = 50), C. parapsilosis ( n = 10), C. glabrata ( n = 10), C. tropicalis ( n = 10), C. guillermondii ( n = 6), C. rugosa ( n = 5), C. krusei ( n = 5), C. kefyr ( n = 2), C. pelliculosa ( n = 2), Saccharomyces cerevisiae ( n = 3), and Geotrichum candidum ( n = 3). The DD assay was performed in supplemented Mueller-Hinton agar with CAS, which was tested at c…

Evaluation of the Disk Diffusion Method Compared to the Microdilution Method in Susceptibility Testing of Anidulafungin against Filamentous Fungi

2008

ABSTRACT Susceptibility testing of anidulafungin (AFG) against 32 mold isolates showed an excellent correlation between disk diffusion (DD) and broth microdilution methods. Based on our data, a 2-μg disk of AFG and a 24-h reading time might represent the best parameters for AFG DD testing against filamentous fungi.

Commemorating World TB Day 2020: “IT’S TIME” — It’s time to End the Global TB Epidemic

2020

Biocompatibility of three new calcium silicate-based endodontic sealers on human periodontal ligament stem cells.

2016

Aim To evaluate the biocompatibility of three calcium silicate-based endodontic sealers, Bioroot BC Sealer (Septodont, Saint-Maur-des-Fosses, France), Endoseal MTA (EndoSeal, Maruchi, Seoul, Korea) and Nano-ceramic Sealer (B&L Biotech, Fairfax, VA, USA) (NCS), on human periodontal ligament stem cells (hPDLSCs). Methodology Human periodontal ligament stem cells were cultured in the presence of various endodontic sealer eluates for 24 h. Cell viability was determined using the MTT assay. Cell death and changes in phenotype induced by the set endodontic sealer eluates were evaluated through flow cytometry. Also, an in vitro scratch wound-healing model was used to determine their effects in cel…

Effect of pH on compressive strength of some modification of mineral trioxide aggregate

2013

Objectives: Recently, it was shown that NanoMTA improved the setting time and promoted a better hydration process which prevents washout and the dislodgment of this novel biomaterial in comparison with WTMA. This study analyzed the compressive strength of ProRoot WMTA (Dentsply), a NanoWMTA (Kamal Asgar Research Center), and Bioaggregate (Innovative Bioceramix) after its exposure to a range of environmental pH conditions during hydration. Study Design: After mixing the cements under aseptic condition and based on the manufacturers' recommendations, the cements were condensed with moderate force using plugger into 9 × 6 mm split molds. Each type of cement was then randomly divided into three…

Study of two MTA cements

2014

Introduction: To determine and compare the pH, conductivity and calcium release of an experimental Portland cement (PE) consisting of trioxid mineral aggregate and a comercially available modified Portland cement (C.P.M.) after 1, 2, 3, 4, 8, 10, 15 and 30 days. Material and Methods: Cements were mixed following the manufacturer's instructions, with a powder: liquid ratio of 3:1. Each cement was placed in 12 PVC tubes 1 mm in diameter and 10 mm in length and allowed to set. Four empty tubes were used as negative controls. Tubes were submerged in plastic flasks containing 10 ml deionized water and stored at 37ºC and 100% humidity. After 1, 2, 3, 4, 8, 10, 15 and 30 days tubes were removed fr…

Domain specific language for securities settlement systems

2012

Actual problems during design, implementation and maintenance of securities settlement systems software are achieving complementarity of several different, connected, asynchronously communicating settlement systems and verification of this complementarity. The aim of this paper is to create domain specific language for modeling of settlement systems and their interactions. Then use models to calculate settlement systems behavior. Specific of settlement systems requires that they perform accordingly to business rules in any situation. This makes use of model checking a very desirable step in development process of settlement systems. Defining a domain specific language and creating editor su…

Data Quality Model-based Testing of Information Systems: the Use-case of E-scooters

2020

The paper proposes a data quality model-based testing methodology aimed at improving testing methodology of information systems (IS) using previously proposed data quality model. The solution supposes creation of a description of the data to be processed by IS and the data quality requirements used for the development of the tests, followed by performing an automated test of the system on the generated tests verifying the correctness of data to be entered and stored in the database. The generation of tests for all possible data quality conditions creates a complete set of tests that verify the operation of the IS under all possible data quality conditions. The proposed solution is demonstra…

Data Quality Model-based Testing of Information Systems

2020

This paper proposes a model-based testing approach by offering to use the data quality model (DQ-model) instead of the program’s control flow graph as a testing model. The DQ-model contains definitions and conditions for data objects to consider the data object as correct. The study proposes to automatically generate a complete test set (CTS) using a DQmodel that allows all data quality conditions to be tested, resulting in a full coverage of DQ-model. In addition, the possibility to check the conformity of the data to be entered and already stored in the database is ensured. The proposed alternative approach changes the testing process: (1) CTS can be generated prior to software developmen…