Search results for "testing"

The nucleosome remodeling factor ISWI functionally interacts with an evolutionarily conserved network of cellular factors

2010

Abstract ISWI is an evolutionarily conserved ATP-dependent chromatin remodeling factor playing central roles in DNA replication, RNA transcription, and chromosome organization. The variety of biological functions dependent on ISWI suggests that its activity could be highly regulated. Our group has previously isolated and characterized new cellular activities that positively regulate ISWI in Drosophila melanogaster. To identify factors that antagonize ISWI activity we developed a novel in vivo eye-based assay to screen for genetic suppressors of ISWI. Our screen revealed that ISWI interacts with an evolutionarily conserved network of cellular and nuclear factors that escaped previous genetic…

2020

In this study, the potential for correlation between disintegration and dissolution performance of enteric-coated (EC) dosage forms was investigated. Different enteric hard shell capsule formulations containing caffeine as model drug were tested for disintegration (in a compendial disintegration tester) and for dissolution in both USP type I (basket) and type II (paddle) apparatuses using different media. Overall, good correlations were obtained. This was observed for both the basket and the paddle apparatus, indicating that the use of disintegration testing as a surrogate for dissolution testing (allowed by International Conference on Harmonization (ICH) for immediate release dosage forms …

Multicomponent solid dispersion as a formulation strategy to improve drug permeation: A case study on the anti-colorectal cancer irinotecan

2019

Abstract Multicomponent solid dispersions (MSD)s are frequently proposed as efficient drug delivery systems to improve drug solubility and bioavailability. In this study, the effects of specific excipients, such as mannitol, inulin, poly(methyl methacrylate-co-methacrylic)acid (PMMA) and cellulose acetate phthalate (CAP) have been tested to potentially improve irinotecan (IRN) permeation in the intestinal tract with the intention to protect the drug from the gastric environment. MSDs were formulated as microparticles by Spray-Drying technique. Raw materials and microparticles have been characterized by FTIR analysis to determine hydrogen bonding. SEM images were recorded to investigate morp…

Semiquantitative multiplex PCR: a useful tool for large rearrangement screening and characterization

2006

Methods presently employed for detection of large rearrangements have several drawbacks, such as the amount of sample and time required, technical difficulty, or the probability of false-negative carriers. Using the low-density-lipoprotein receptor (LDLR) gene, whose mutations are responsible for familial hypercholesterolemia (FH), we have developed a procedure to detect large rearrangements in this gene based on semiquantitative PCR, with important improvements as compared to previous methods. Our method covers the complete LDLR gene and introduces an internal control in the reaction. The procedure discriminates the four different large rearrangements (two deletions and two insertions) tha…

Relationship status and respiratory diseases: an analysis on a general population sample

2021

Background: Very few studies have examined whether marital/relationship status affects respiratory diseases. Objective: Assess the association of relationship status (Rstatus: domestic partnership/civil union/marriage, cohabitation with non-partners, being single) with current (CA) and past asthma (PA), allergic (AR) and non-allergic rhinitis (NAR), and chronic bronchitis (CB). Methods: Data were collected in the frame of GEIRD, a population-based, multicase-control survey. Among 2531 subjects (age 21–86, female 50%) who underwent standardized interviews, skin prick and lung function tests, 575 cases of CA, 268 cases of PA, 968 cases of AR, 462 cases of NAR, 265 cases of CB without airflow …

Calorimetric and structural investigation of the interaction between bovine serum albumin and high molecular weight dextran in water.

2005

This work studies specific interactions between a small globular protein and a highly flexible, branched polysaccharide using differential scanning calorimetry (DSC), circular dichroism (CD), fluorescence, and turbidimetry measurements. It uses the system water/bovine serum albumin (BSA)/dextran (D 2000) as a model. Dextran molecules are able to form interpolymeric complexes with BSA in water at both low and high temperatures if the polysaccharide is in excess and if the protein exists in its associated state. It leads to a partial destabilization of the secondary and tertiary structures of the protein and an additional exposure of the hydrophobic tryptophan residues to the surface of globu…

A new paradigm for pattern classification: Nearest Border Techniques

2013

Published version of a chapter in the book: AI 2013: Advances in Artificial Intelligence. Also available from the publisher at: http://dx.doi.org/10.1007/978-3-319-03680-9_44 There are many paradigms for pattern classification. As opposed to these, this paper introduces a paradigm that has not been reported in the literature earlier, which we shall refer to as the Nearest Border (NB) paradigm. The philosophy for developing such a NB strategy is as follows: Given the training data set for each class, we shall first attempt to create borders for each individual class. After that, we advocate that testing is accomplished by assigning the test sample to the class whose border it lies closest to…

87 Topical Seminar Summary: QUANTITATIVE SENSORY TESTING (QST) IN PAIN MEDICINE: WHAT IS ITS VALUE IN CLINICAL PRACTICE?

2006

Adaptive trial design: a general methodology for censored time to event data.

2008

Adaptive designs allow a clinical trial design to be changed according to interim findings without inflating type I error. The Inverse Normal method can be considered as an adaptive generalization of classical group sequential designs. The use of the Inverse Normal method for censored survival data was demonstrated only for the logrank statistic. However, the logrank statistic is inefficient in the presence of nuisance covariates affecting survival. We demonstrate, how the Inverse Normal method can be applied to Cox regression analysis. The required independence between test statistics of the different stages of the trial can be obtained by two different approaches. One is using the indepen…

V: p-Werte: Was sie besagen und was nicht …

2002

Both an extensive data description and an explicit assessment of a study result's statistical significance should be presented in the result section of a clinical trial report. Whereas the description illustrates the order and clinical relevance of the study findings, the statistical significance describes its generalizability to patients not included in the clinical trial: Despite the random recruitment of patients into a trial, the study results may fail to represent clinical reality (for example the trial might show falsely positive efficacy findings, whereas in "clinical reality" efficacy appears rather limited). A p value measures the statistical significance of a study result -- the s…