Search results for "trichoderma"
showing 10 items of 47 documents
The use of glyphosate as the sole source of phosphorus or carbon for the selection of soil-borne fungal strains capable to degrade this herbicide
1997
Abstract The herbicide glyphosate was used as a selection agent for isolation of fungal strains capable to degrade phosphorus-to-carbon bond from standard sandy-clay soil. The studies have shown that the herbicide used in Martin medium as a sole source of phosphorus br carbon caused the decrease of the fungal population and substantially changed strain composition, thus selecting those which are able to degrade glyphosate.
Cork Taint of Wines: Role of the Filamentous Fungi Isolated from Cork in the Formation of 2,4,6-Trichloroanisole by O Methylation of 2,4,6-Trichlorop…
2002
ABSTRACT Cork taint is a musty or moldy off-odor in wine mainly caused by 2,4,6-trichloroanisole (2,4,6-TCA). We examined the role of 14 fungal strains isolated from cork samples in the production of 2,4,6-TCA by O methylation of 2,4,6-trichlorophenol (2,4,6-TCP). The fungal strains isolated belong to the genera Penicillium (four isolates); Trichoderma (two isolates); and Acremonium , Chrysonilia , Cladosporium , Fusarium , Mortierella , Mucor , Paecilomyces , and Verticillium (one isolate each). Eleven of these strains could produce 2,4,6-TCA when they were grown directly on cork in the presence of 2,4,6-TCP. The highest levels of bioconversion were carried out by the Trichoderma and Fusar…
Geni indotti da mercurio in Trichoderma harzianum
2011
Il lavoro della presente tesi ha riguardato lo studio della capacità di Trichoderma harzianum di crescere in presenza di mercurio. L’obiettivo principale era quello di comprendere i meccanismi biomolecolari coinvolti nella risposta metabolica allo stress indotto da mercurio in T. harzianum. E’ stato studiato l’accrescimento radiale su piastra in terreno colturale agarizzato in presenza di cadmio e mercurio e il peso secco del micelio del fungo accresciutosi in terreno colturale liquido nelle medesime condizioni. In entrambi i casi T. harzianum ha mostrato una maggiore capacità di crescita in presenza di mercurio. In un secondo tempo, la ricerca ha avuto come obiettivo quello di identificare…
Expression and Secretion of Barley Cysteine Endopeptidase B and Cellobiohydrolase I in Trichoderma reesei.
2006
Localization of expression and secretion of a heterologous barley cysteine endopeptidase (EPB) and the homologous main cellobiohydrolase I (CBHI) in a Trichoderma reesei transformant expressing both proteins were studied. The transformant was grown on solid medium with Avicel cellulose and lactose to induce the cbh1 promoter for the synthesis of the native CBHI and the recombinant barley protein linked to a cbh1 expression cassette. Differences in localization of expression between the two proteins were clearly indicated by in situ hybridization, indirect immunofluorescence, and immunoelectron microscopy. In young hyphae, native-size recombinant epb mRNA was localized to apical compartments…
Maturation of barley cysteine endopeptidase expressed in Trichoderma reesei is distorted by incomplete processing
2002
Maturation of barley cysteine endopeptidase B (EPB) in Trichoderma reesei was studied with metabolic inhibitors, Western blotting, and immuno microscopy. The inactive 42-kDa recombinant EPB proprotein, first detected in apical cells, was sequentially processed in a time-dependent manner to a secreted polypeptide of 38.5 kDa, and thereafter, to polypeptides of 37.5, 35.5, and 32 kDa exhibiting enzyme activity both in the hyphae and culture medium. The sizes of the different forms of recombinant EPB were in accordance with molecular masses calculated from the deduced amino acid sequence, assuming cleavage at four putative Kex2p sites present in the 42-kDa proprotein. Both the liquid and the z…
Application of Toxkit microbiotests for toxicity assessment in soil and compost
2004
The potential of Toxkit microbiotests to detect and analyze pollution in agricultural soil and the quality of compost was studied. The toxicity tests used included seed germination biotests using cress salad (Lepidum sativum L.), tomato (Lycopersicum esculentum L.), and cucumber (Cucumis sativus L.), and the Toxkit microbiotests included those with microalgae (Selenastrum capricornutum), protozoa (Tetrahymena thermophila), crustaceans (Daphnia magna, Thamnocephalus platyurus, and Heterocypris incongruens), and rotifers (Brachionus calyciflorus). Experiments on compost were undertaken in a modified solid-state fermentation system (SSF) and under field conditions (in a windrow). To promote th…
Growth and Antagonism of <i>Trichoderma</i> spp. and Conifer Pathogen <i>Heterobasidion annosum</i&a…
2012
Variations in the radial growth rate of 24 isolates belonging to ten species of Trichoderma, three isolates of conifer pathogen Heterobasidion annosum s.s. and four isolates of H. parviporum were evaluated by incubation on a solid malt extract medium at a temperature of 4℃, 15℃ and 21℃. Trichoderma antagonism against Heterobasidion was investigated in dual culture in vitro. The slowest rate of growth was referable to all seven strains of Heterobasidion spp. All Heterobasidion spp. strains were overgrown by 63% of Trichoderma spp. strains after two weeks at 21℃ and by 33% of strains at 15℃. 21% of Trichoderma strains did not grow and only four strains belonging to T. koningii, T. viride and …
Enzymes for the NADPH-dependent reduction of dihydroxyacetone and D-glyceraldehyde and L-glyceraldehyde in the mould Hypocrea jecorina
2006
The mould Hypocrea jecorina (Trichoderma reesei) has two genes coding for enzymes with high similarity to the NADP-dependent glycerol dehydrogenase. These genes, called gld1 and gld2, were cloned and expressed in a heterologous host. The encoded proteins were purified and their kinetic properties characterized. GLD1 catalyses the conversion of d-glyceraldehyde and l-glyceraldehyde to glycerol, whereas GLD2 catalyses the conversion of dihydroxyacetone to glycerol. Both enzymes are specific for NADPH as a cofactor. The properties of GLD2 are similar to those of the previously described NADP-dependent glycerol-2- dehydrogenases (EC 1.1.1.156) purified from different mould species. It is a reve…
Infection pulmonaire invasive à Trichoderma longibrachiatum, à propos d’un cas
2015
Introduction : Trichoderma longibrachiatum est un champignon filamenteux appartenant à la famille des hyalohyphomycètes, rarement isolé lors d’infections fongiques invasives chez l’Homme mais quelques cas ont déjà été décrits chez des sujets immunodéprimés. Observation : Un homme de 69 ans a développé un syndrome fébrile une semaine après la fin d’une chimiothérapie pour le traitement d’une leucémie aiguë myéloïde alors qu’il était en aplasie profonde (GB Aspergillus Galactomannan test), un scanner thoracique est réalisé. L’aspect scannographique (signe du halo) était évocateur d’une aspergillose pulmonaire invasive (API), motivant la réalisation d’un lavage broncho-alvéolaire (LBA). Le LBA…
Antimicrobial Properties of the Modified Cotton Textiles by the Sol-Gel Technology
2015
Comparison of the antimicrobial properties of textiles with amorphous coatings containing Zn and Si obtained by the sol-gel technology against microorganismsPseudomonas fluorescens,Saccharomyces cerevisae,Trichoderma viridethat can cause cotton textile destruction was made. Modified textile were evaluated using scanning electron microscopy, energy dispersive x-ray spectroscopy. Investigation of antimicrobial properties of the cotton fabric samples treated with different zinc acetate dihydrate concentration sols and various thermal post-treatments was made. Strong inhibition of microbial growth was detected for all cotton textile samples treated via sol-gel method according all tests microor…