Search results for "uci"

A Novel Allylic Anchor for Solid-Phase Synthesis—Synthesis of Protected and Unprotected O-Glycosylated Mucin-Type Glycopeptides

1995

Cell Surface-Bound Leucine Aminopeptidase: Target of the Immunomodulator Bestatin

1986

The study of low molecular weight enzyme inhibitors of microbial origin was initiated by Umezawa in 1965 (see Umezawa 1972). Since the discovery of an inhibitor of tyrosine hydroxylase, nearly 50 inhibitors of various enzymes have been found by him; their structures were elucidated and most of the compounds were chemically synthesized (Umezawa 1982). Among them one inhibitor of both aminopeptidase B and the ectoenzyme, leucine aminopeptidase was found in 1976 and was termed bestatin (Fig. 1), [(2S,3R)-3-amino-2-hydroxy 4-phenyl-butanoyl]-(S)-leucine (Umezawa et al. 1976).

Amino acid contents of infant foods.

2006

The protein quality of three milk-cereal-based infant foods (paps) was evaluated by determining their amino acid contents and calculating the amino acid score. Proteins were subjected to acid hydrolysis, prior to which cysteine and methionine were oxidized with performic acid. Amino acids were determined by reverse-phase high-performance liquid chromatography with fluorescence detection with a prior derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate. Tryptophan was determined by reverse-phase high-performance liquid chromatography with ultraviolet detection after basic hydrolysis. Glutamic acid, proline and leucine were the most abundant amino acids, whereas tryptophan and …

Transport-related amino acid metabolism in germinating barley grains

1987

When eight [14C]-labelled amino acids were separately injected into the endosperm of germinating (4 days at 20°C) barley (Hordeum vulgare L. cv. Himalaya) grains, the label was rapidly taken up by the scutellum and further transported to the shoot and roots. Some of the amino acids (leucine, lysine and asparagine) were transported in an intact form through the scutellum to the seedling, whilst glutamic acid and aspartic acid were largely converted to glutamine in the scutellum. Proline was mainly transported unchanged, but a small part of the label appeared in glutamine. Arginine was mostly broken down in the scutellum, possibly providing ammonia for the synthesis of glutamine. During furth…

Oxygen Availability, Energy Metabolism, and Metabolic Rate in Invertebrates and Vertebrates

1988

It has often been emphasized that primitive life originated in an environment devoid of oxygen. The first eukaryotic cells, however, appeared some 1.4 billion years ago when the earth’s atmosphere had already turned from a mildly reducing to an oxidizing one by the photosynthetic action of prokaryotes that used H20 as reducing agent (see Harold 1986, for review). The presence of free oxygen obviously was a major force shaping the evolution of eukaryotic cells. As a consequence all animals are primarily aerobes, using respiratory chains with oxygen as electron acceptor (oxidant) and membrane-bound ATP synthases for the production of ATP.

Semiquantitative bioluminescent assay of glutathione

1998

A novel technique has been developed for semiquantitative detection of glutathione (GSH) in small volumes of liquid samples. GSH is detected via enzymatic linkage to the NADP/NADPH + H+ redox system through glutathione reductase. Accumulated NADPH is measured via the bioluminescent FMN oxidoreductase bacterial luciferase reaction. A linear correlation is obtained between bioluminescence intensity of the luciferase reaction and the GSH content of the liquid sample. Possible applications of this procedure are discussed. © 1998 John Wiley & Sons, Ltd.

Tocopherol, fatty acid and phytosterol content in seeds of nine wild taxa of Sicilian Brassica (Cruciferae)

2010

The purpose of this study was to determine tocopherol (Toc), fatty acid and phytosterol content in dry seeds of nine wild taxa of Sicilian Brassica sect. Brassica. Analyses were performed by gas chromatography (GC)-mass spectroscopy (MS). The biochemical analysis of dry seeds showed that in the nine taxa examined, α - and γ -Toc were the major isomers, δ - Toc was present in traces and β -Toc was never found. All taxa had a similar fatty acid composition with C 16:0 (357.2 mg kg − 1 DW), C 18:1 (393.94 mg kg − 1 DW) and C 22:1 (618.29 mg kg − 1 DW) acids present in higher amount. Unsaturated fatty acids (52.5–66.5%) were more abundant than saturated (33.5–47.5%) ones relative to the total f…

SF002-96-1, a new drimane sesquiterpene lactone from an Aspergillus species, inhibits survivin expression

2013

Survivin, a member of the IAP (inhibitor of apoptosis) gene family, is overexpressed in virtually all human cancers and is functionally involved in the inhibition of apoptosis, regulation of cell proliferation, metastasis and resistance to therapy. Because of its upregulation in malignancy, survivin has currently attracting considerable interest as a new target for anticancer therapy. In a screening of approximately 200 strains of imperfect fungi for the production of inhibitors of survivin promoter activity, a new drimane sesquiterpene lactone, SF002-96-1, was isolated from fermentations of an Aspergillus species. The compound inhibited survivin promoter activity in transiently transfected…

Absence of binding of human salivary glycoprotein to human gingival fibroblast-like cells in vitro.

1996

The aim of this study was to determine whether human high molecular weight salivary glycoprotein binds in vitro to human gingival fibroblast-like cells. Primary monolayer cultures of 2 human gingival fibroblast-like cell lines were incubated with a high molecular weight fraction of salivary glycoprotein which expressed blood group A activity and glycoprotein-cell binding probed using an FITC-conjugated mouse monoclonal antibody to human blood group A antigen. Surface fluorescence of protein-treated cells was found to be no greater than that of untreated or serum-treated control cultures. As significant binding of salivary glycoprotein to gingival fibroblast-like cells does not occur in vitr…

Increased sulfate availability in saline water promotes hydrogen sulfide production in fish organic waste

2020

The risk of hydrogen sulfide (H2S) production can be a challenge in marine land-based recirculating aquaculture systems (RAS). Hydrogen sulfide is a toxic gas that can cause massive fish mortality even at low concentrations, and in addition, serious odour problems in the surroundings. It is a bacterial by-product originating from the degradation of organic matter in sulfur-rich waters such as marine waters. In order to hinder H2S production in marine land-based RAS, more information on the H2S production conditions and the associated microbiology is needed. In this study, the production of H2S from rainbow trout (Oncorhynchus mykiss) organic waste was examined using a novel H2S measurement …