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showing 10 items of 6927 documents
Rational drug discovery : structural studies of protein-ligand complexes
2011
Antibacterial activity and cytotoxicity of selected Egyptian medicinal plants.
2011
Medicinal plants have been used as a source of remedies since ancient times in Egypt. The present study was designed to investigate the antibacterial activity and the cytotoxicity of the organic extracts from 16 selected medicinal plants of Egypt. The study was also extended to the isolation of the antiproliferative compound jaeschkeanadiol p-hydroxybenzoate (FH-25) from Ferula hermonis. The microbroth dilution was used to determine the minimal inhibitory concentration (MIC) of the samples against twelve bacterial strains belonging to four species, Providencia stuartii, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Escherichia coli, while a resazurin assay was used to assess the cytoto…
In-Depth Characterization of Bioactive Extracts from Posidonia oceanica Waste Biomass
2019
© 2019 by the authors.
Att skapa och upprätthålla förtroendeförhållanden i vardagen
2012
Etnologinen tutkimus palvelujen käytöstä ja hyvän arjen rakentumisesta
2014
Lipīdu profila raksturojums un to atbilstība vadlīniju mērķiem koronārās sirds slimības pacientiem, kas stacionēti Latvijas Kardioloģijas centrā
2018
Ievads: Kardiovaskulārās slimības (KVS) mirstības ziņā joprojām ieņem pirmo vietu pasaulē. Latvijā 2014. gadā tās veidoja 64% no visiem nāves cēloņiem sievietēm un 49% no visiem nāves cēloņiem vīriešiem (OECD, 2017). Jo īpaši, zema blīvuma lipoproteīnu holesterīns (ZBLH) ir galvenais terapeitiskais mērķis, aizkavējot saslimšanu ar KVS. Nesen papildinātās dislipidēmijas ārstēšanas vadlīnijas nosaka ļoti augsta riska pacientiem ZBLH mērķa līmeni <1,8 mmol/L un sākotnējā ZBLH līmeņa pazemināšanu vismaz par 50%. Mērķis: Analizēt lipīdu mērķa līmeņu sasniegšanas biežumu – ZBLH <1,8 mmol/L un ne - ABLH <2,6 mmol/L, un analizēt lipīdus pazeminošo līdzekļu (LPL) lietošanu starp pacientiem ar ļoti a…
Peptide-mediated interference with baculovirus transduction
2007
Baculovirus represents a multifunctional platform with potential for biomedical applications including disease therapies. The importance of F3, a tumor-homing peptide, in baculovirus transduction was previously recognized by the ability of F3 to augment viral binding and gene delivery to human cancer cells following display on the viral envelope. Here, F3 was utilized as a molecular tool to expand understanding of the poorly characterized baculovirus-mammalian cell interactions. Baculovirus-mediated transduction of HepG2 hepatocarcinoma cells was strongly inhibited by coincubating the virus with synthetic F3 or following incorporation of F3 into viral nucleocapsid by genetic engineering, th…
Occlusion-derived baculovirus: interaction with human cells and evaluation of the envelope protein P74 as a surface display platform.
2008
To develop complementary baculovirus-based tools for gene delivery and display technologies, the interaction of occlusion-derived baculovirus (ODV) with human cells, and the functionality of the P74 ODV envelope protein for display of the IgG-binding Z domains (ZZP74) were evaluated. The cellular binding of ODV was concentration-dependent and saturable. Only minority of the bound virions were internalized at both 37 and 4 degrees C, suggesting usage of direct membrane fusion as the entry mode. The intracellular transport of ODV was confined in vesicular structures peripheral to the plasma membrane, impeding subsequent nuclear entry and transgene expression. Transduction of ODV was not rescu…
Internalization of novel non-viral vector TAT-streptavidin into human cells
2007
BMC Biotechnology, 7 (1)
Baculovirus display strategies: Emerging tools for eukaryotic libraries and gene delivery
2004
Recombinant baculoviruses have been extensively used as vectors for abundant expression of a large variety of foreign proteins in insect cell cultures. The appeal of the system lies essentially in easy cloning techniques and virus propagation combined with the eukaryotic post-translational modification machinery of the insect cell. Recently, a novel molecular biology tool was established by the development of baculovirus surface display, using different strategies for presentation of foreign peptides and proteins on the surface of budded virions. This eukaryotic display system enables presentation of large complex proteins on the surface of baculovirus particles and has thereby become a ver…