Search results for "vidi"
showing 10 items of 346 documents
Un "falso" ovidiano del XIII secolo: gli «Pseudo-Remedia amoris»
2011
Ovidio nel mondo ispanico. Influenze, echi, riscritture.
2020
Studio sull'influenza del poeta Ovidio nella letteratura del mondo ispanico, tra tradizione e modernità
Trombofilia ereditaria e complicanze gravidiche nella popolazione siciliana
2009
Trombofilia ereditaria e complicanze gravidiche nella popolazione siciliana. È ormai noto che la trombofilia congenita rappresen- ti un fattore di rischio per la gravidanza. Può infatti es- sere causa di abortività ricorrente, perdita fetale (MEF), ritardo di crescita fetale (IUGR) e altri problemi nel mantenimento della gravidanza (preeclampsia e di- stacco di placenta). Nel presente studio è stata indaga- ta la prevalenza dei fattori trombofilici nella popolazio- ne siciliana e la possibile relazione fra essi ed un even- to trombotico uteroplacentare in gravidanza. Sono sta- ti comparati due gruppi di gestanti con anamnesi posi- tiva per presenza di almeno un fattore trombofilico e storia…
Scritti in onore di Pellegrino Capaldo
2014
La qualità dei servizi è stata riconosciuta come un rilevante aspetto caratterizzante la performance dell’azienda pubblica, analogamente alla capacità di operare secondo criteri di efficienza e nel rispetto dei vincoli di bilancio. Sebbene sia possibile riscontrare alcuni significativi casi di adozione di politiche di customer satisfaction nelle amministrazioni pubbliche italiane, a tutt’oggi il tema presenta significative criticità, specialmente in ordine agli aspetti metodologici e agli strumenti operativi che consentano di integrare dette politiche nel sistema di programmazione e controllo delle aziende che erogano tali servizi. Il presente lavoro adotta una diversa prospettiva di analis…
Literatura ovidiana (Ars Amandi y Reprobatio amoris) en la educación medieval
2004
La tradición de las obras ovidianas, sobre todo su Ars amandi y Reprobatio amoris, fueron una fuente inagotable de inspiración para gran parte de los autores medievales y renacentistas. Se analiza en este artículo las razones de su utilización como material docente en los siglos XII y XIII, y como se fusionaron ambos textos con la tradición cristiana (santos Padres de la Iglesia, autores monacales, etc.) y con la filosofía moral aristotélica, dando nuevos modelos de comportamiento amoroso. -canet@uv.es
Efficient production of active chicken avidin using a bacterial signal peptide in Escherichia coli
2004
Chicken avidin is a highly popular tool with countless applications in the life sciences. In the present study, an efficient method for producing avidin protein in the periplasmic space of Escherichia coli in the active form is described. Avidin was produced by replacing the native signal sequence of the protein with a bacterial OmpA secretion signal. The yield after a single 2-iminobiotin–agarose affinity purification step was approx. 10 mg/l of virtually pure avidin. Purified avidin had 3.7 free biotin-binding sites per tetramer and showed the same biotin-binding affinity and thermal stability as egg-white avidin. Avidin crystallized under various conditions, which will enable X-ray cryst…
Real time optical immunosensing with flow-through porous alumina membranes
2014
Through the presentation of analytical data from bioassay experiments, measured by polarimetry, we demonstrate for the first time a real time immunoassay within a free standing macroporous alumina membrane. The 200 nm nominal pore diameter of the membrane enables flow-through, thereby providing an ideal fluidic platform for the targeted delivery of analytes to bioreceptors immobilized on the pore walls, enabling fast sensing response times and the use of small sample volumes (<100 mu L). For the immunoassay, the pore walls were first coated with the functional copolymer, copoly(DMA-NAS) using a novel coupling process, before immobilization of the allergen protein, beta-lactoglobulin, by spo…
Recent advances in surface plasmon resonance biosensors for microRNAs detection
2020
miRNAs are a large family of non-coding RNAs which play important roles in translational and post-transcriptional regulation of gene expression and biological processes. Abnormal expression of miRNAs is related to the initiation and progression of different diseases which make them be promising candidates for early medical diagnostics. Thus, accurate detection of miRNAs has great significance for disorder diagnosis. Nevertheless, their intrinsic characteristics such as short sequence, low concentration and sequence homology challenge routine techniques. The detection assays need to be extremely sensitive and selective in small value of intricate RNA samples. Biosensor-based strategies have …
High-resolution crystal structure of an avidin-related protein: insight into high-affinity biotin binding and protein stability.
2004
The chicken avidin gene belongs to an extended gene family encoding seven avidin-related genes (AVRs), of which only avidin is expressed in the chicken. The sequences of AVR4 and AVR5 are identical and the common protein (AVR4) has been expressed both in insect and bacterial systems. The recombinant proteins are similarly hyperthermostable and bind biotin with similarly high affinities. AVR4 was crystallized in the apo and biotin-complexed forms and their structures were determined at high resolution. Its tertiary and quaternary structures are very similar to those of avidin and streptavidin. Its biotin-binding site shows only a few alterations compared with those of avidin and streptavidin…
Specific interaction of desthiobiotin lipids and water-soluble biotin compounds with streptavidin
1991
As shown for biotin lipids (Ref. 1), the formation of perfect 2-D crystalline streptavidin domains can also be observed in the plane of desthiobiotin lipid monolayers. The binding constant of streptavidin with desthiobiotin (Ka = 5·1013 mol−1) is lower than that with biotin (Ka = 1015 mol−1) (Ref. 2). By adding free biotin into the subphase a competitive replacement and a detaching of the streptavidin domains from the desthiobiotin lipid monolayer takes place. Streptavidin domains built at receptor lipid monolayers are still functional. As could be shown, there are two biotin binding sites at each protein molecule that are fully accessible to biotin (Ref. 1). This can be proven by the inter…