Search results for "vidi"

Selective targeting of avidin/mannose 6-phosphate receptor chimeras to early or late endosomes

2000

Summary In this study we have used the Semliki forest virus expression system to transiently express chimeric proteins that contain transmembrane and cytoplasmic domains of the cation-independent mannose 6-phosphate receptor (CI-MPR) fused to chicken avidin. Immunofluorescence and electron microscopy studies showed that the chimeric protein with the entire cytoplasmic domain of CI-MPR was transported to late endosomes, where it accumulated. We made use of the biotin-binding capacity of lumenal avidin, and found that, in agreement with this distribution, the chimeric protein could be labelled with biotinylated HRP endocytosed for a long, but not a brief, period of time. However, truncation o…

Dual-affinity avidin molecules

2005

A recently reported dual-chain avidin was modified further to contain two distinct, independent types of ligand-binding sites within a single polypeptide chain. Chicken avidin is normally a tetrameric glycoprotein that binds water-soluble d-biotin with extreme affinity (Kd ≈ 10−15M). Avidin is utilized in various applications and techniques in the life sciences and in the nanosciences. In a recent study, we described a novel avidin monomer-fusion chimera that joins two circularly permuted monomers into a single polypeptide chain. Two of these dual-chain avidins were observed to associate spontaneously to form a dimer equivalent to the wt tetramer. In the present study, we successfully used …

Determinants of COVID-19 Vaccine Rollouts and Their Effects on Health Outcomes

2022

Background Vaccination against the coronavirus disease (SARS-CoV-2) is understood to be the key way out of the COVID-19 pandemic. Limited evidence exists on the determinants of vaccine rollouts and their health effects at the country level. Objective Examine the determinants of COVID-19 vaccine rollouts and their effects on health outcomes. Methods Ordinary least squares regressions with standard errors clustered at the country level for Cross-section and Panel daily data of vaccinations and various health outcomes (new COVID-19 cases, fatalities, intensive care unit (ICU) admissions) for an unbalanced sample of about 200 countries during the period 16 December 2020 to 20 June 2021. Results…

Likviditātes vadības politika Eirosistēmā

2018

Tradicionālo monetārās politikas instrumentu izmantošana pierādījās nepietiekami efektīva globālās finanšu krīzes radīto ārkārtējo apstākļu uzlabošanai, tāpēc Eiropas Centrālā banka no 2007. gada sāka izmantot nestandarta monetārās politikas metodes, lai stabilizētu situāciju finanšu tirgos. No 2009. gada Eiropas Centrālā banka sāka izmantot aktīvu pirkšanas programmas funkcionalitātes nodrošināšanai noteiktos finanšu tirgus segmentos, monetārās politikas transmisijas veicināšanai, kopš 2015. gada ar mērķi veicināt ekonomisko aktivitāti un pacelt inflācijas līmeni līdz noteiktajam mērķim “tuvu, bet zem 2%” vidējā termiņā. Pētījuma mērķis ir, novērtējot ECB likviditātes vadības politiku, tās…

Investīcijas un investīciju vide Latvijā

2021

Autore darba gaitā aplūko investīciju vidi ietekmējošos faktorus, atspoguļojot aktuālo pētījumu rezultātus un tendences investīciju vides izpētes jomā, detalizēti analizējot investīciju vidi Latvijā. Darba gaitā autore analizē zinātnisko literatūru gan Latvijas, gan starptautisko autoru pētījumus, interneta resursus, lai sniegtu plašu priekšstatu par investīciju vides būtību, to veidojošiem un ietekmējošiem elementiem. Darba trešajā daļā, autore piedāvā analīzi, kas sniedz priekšstatu par investīciju vidi veidojošo faktoru ietekmi. Darba Investīcijas un investīciju vide Latvijā darba mērķis ir: balstoties uz teorētiskās bāzes izpēti, analizēt un apkopot informāciju par investīcijām, to vidi…

Avidin Is a Promising Tag for Fusion Proteins Produced in Baculovirus-Infected Insect Cells

1999

The baculovirus expression vector system (BEVS) has become one of the most versatile and powerful eukaryotic systems for recombinant protein expression. We have constructed a novel baculovirus transfer vector (pbacAVs+C) which allows for the efficient production, detection, and single-step purification of the desired molecule as a secretion-compatible avidin fusion protein in insect cells. It also enables fast construction of the baculoviruses by site-specific transposition in Escherichia coli. To demonstrate the power of this vector, we report here on the production of immunologically intact hevein, a major cysteine-rich latex allergen, as avidin fusion protein. Our results indicate that a…

Construction of an agglutination tool: recombinant Fab fragments biotinylated in vitro.

2009

The pComb3H vector system is used for constructing and panning recombinant antibody libraries. It allows for expression of monovalent Fab fragments, either on the surface of M13 phage, or in the form of soluble proteins secreted into the periplasmic space of bacteria. We constructed a modified pComb3H vector containing cDNA encoding for a 23-amino acid fragment of the Escherichia coli biotin carboxy carrier protein (BCCP), which is an acceptor sequence for biotinylation. The vector was used to express the Fab fragment recognizing human glycophorin A. The purified Fab fragment containing this biotin acceptor sequence was effectively biotinylated in vitro using biotin ligase (BirA). The speci…

Rec. a Helen Van Noorden, Playing Hesiod. The ‘Myth of the Races’ in Classical Antiquity, Cambridge, Cambridge University Press, 2015, x+350 p., 72 l…

2017

Recensione a Helen Van Noorden, Playing Hesiod. The ‘Myth of the Races’ in Classical Antiquity, Cambridge 2015

L'autorità e le sue contraddizioni: Numa nei Fasti di Ovidio

2008

This article shows how the few, but significant negative aspects of the figure of Numa in the historiographical tradition are dealt with in the Fasti. The ideological and broadly cultural implications of the ovidian treatment of the Numa character are explored, especially in relation with his discourse on the Einzelherrschaft in the late Augustan age

Motivi tragici nelle Imagines di Filostrato Maggiore (I 4; 18; 27; II 4; 10; 13; 23; 29; 30).

Grazie all'analisi del rapporto tra il testo delle descrizioni di Filostrato e quello di Seneca tragico, Ovidio e Stazio si ipotizza la presenza nelle Imagines di allusioni ad opere ellenistiche perdute (si fa riferimento a Callimaco e Nicandro) . Inoltre, sulla base di un parallelismo tra II 4 (Ippolito) e un verso delle Rane di Aristofane si rintraccia una citazione dell'Ippolito Velato euripideo. The analysis of the relationship between the Philostratean text and the one of Seneca (only Tragedies), Ovid and Statius confirmed the presence in Philostratus' Imagines of allusions to some Hellenistic lost works (we refer to Callimachus and Nicander) . In addition, thanks to a parallel between…