Search results for "virus"

The complete nucleotide sequence of an isolate of Tomato yellow leaf curl Sardinia virus found in Sicily

2010

Partial sequences of Tomato yellow leaf curl Sardinia virus (TYLCSV) derived from tomato samples collected in Sicily in 1999, 2002 and 2004 indicated the presence of a TYLCSV different from the one previously described as the Sic strain. Here, we report a complete DNA sequence that is classified as belonging to the TYLCSV type strain (Sar strain), confirming the co-existence in Sicily of virus populations of both strains. Moreover, comparisons between this new sequence and those of the two recombinants recently described in Sicily revealed unequivocally (99% identity) that their TYLCSV-derived portion originated from the Sar strain. © 2010 Springer-Verlag.

Real-time RT-PCR detection of betanodavirus in naturally and experimentally infected fish from Spain

2011

Infections with betanodavirus affect a wide range of wild and farmed fish species throughout the world, mostly from the marine environment. The aim of this work was to develop and validate real-time RT-PCR assays for sensitive and specific detection of nodavirus in diseased or carrier fish. The new detection assay was used to study the transmission and development of nodavirus infection in juvenile sea bass, Dicentrarchus labrax (L.), challenged by different routes, and also to screen for nodavirus in various farmed fish species. On average, the sensitivity was 10-100 times higher than a standard RT-PCR, and the assay was able to detect asymptomatic carrier fish that otherwise could have be…

Comparative evolution of P–M system and infection by the sigma virus in French and Spanish populations ofDrosophila melanogaster

1992

SummaryIn 1983, an extensive survey of populations ofD. melanogasterwas started in a southern French region (Languedoc) in two non-Mendelian systems: the P–M system of transposable elements and the hereditary Rhabdovirus sigma. Unexpectedly fast-evolving phenomena were observed and interesting correlations were noted, giving similar geographical pattern to the region in both systems. For these reasons, the analysis was continued and extended towards the north (Rhône Valley) and the south (Spain). In the P–M system, all the Languedoc populations evolved from 1983 to 1991 towards the Q type which is characteristic of the Rhône Valley populations. In contrast, M′ strains are currently observed…

Pathogenese und Diagnostik der Cytomegalovirus-Infektion

2008

S3-Leitlinie „Prophylaxe, Diagnostik und Therapie der Hepatitis-C-Virus (HCV) -Infektion“ : AWMF-Register-Nr.: 021/012

2018

Viral diseases causing the greatest economic losses to the tomato crop. I. The Tomato spotted wilt virus — a review

1996

Abstract Tomato spotted wilt virus (TSWV) is one of the most important diseases affecting tomatoes, occasionally leading to losses of up to 100%. Because of the importance of this virus disease, considerable research has been carried out in recent years on the viral particle, disease vectors, transmission and control methods. This review presents an account of the current state of research on TSWV with special reference to tomato. Genetic resistance appears as the best solution to control this disease. It may be complemented with other strategies of control aimed to reduce inoculum amount or to avoid transmission, since these strategies have been shown to be ineffective to stop the disease …

SARS-CoV-2 RNA and antibody detection in human milk from a prospective multicenter study in Spain

2021

Background During the COVID-19 pandemic in 2020, breastfeeding in women positive for SARS-CoV-2 was compromised due to contradictory data regarding potential viral transmission. However, growing evidence confirms the relevant role of breast milk in providing passive immunity by generating and transmitting specific antibodies against the virus. Thus, our study aimed to develop and validate a specific protocol to detect SARS-CoV-2 in breast milk matrix as well as to determine the impact of maternal SARS-CoV-2 infection on presence, concentration, and persistence of specific SARS-CoV-2 antibodies. Study design/Methods A prospective multicenter longitudinal study in Spain was carried out from A…

Viral diseases causing the greatest economic losses to the tomato crop. II. The Tomato yellow leaf curl virus — a review

1996

Abstract Tomato yellow leaf curl geminivirus (TYLCV), transmitted by the whitefly Bemisia tabaci (Gennadius) is one of the most devastating diseases of cultivated tomato (Lycopersicon esculentum Mill.). TYLCV causes economic losses up to 100% in tomato crop in many tropical and subtropical regions, and is spreading towards new areas. The increasing economic importance of TYLCV has resulted in the need for accurate detection and identification procedures, stimulating intensive research efforts focused on virus biology, diversity, and epidemiology to develop successful control strategies. Breeding for resistance appears to be the best approach to control this disease, but to date only partial…

Pityriasis lichenoides chronica associated with herpes simplex virus type 2.

2012

Introduction. Pityriasis lichenoides is a rare, acquired spectrum of skin conditions of an unknown etiology.Case Report. A 28-year-old man presented with recurrent outbreaks of herpes simplex virus associated with the onset of red-to-brown maculopapules located predominantly in trunk in each recurrence. Positive serologies to herpes simplex virus type 2 were detected. Histopathological examination of one of the lesions was consistent with a diagnosis of pityriasis lichenoides chronica.Discussion. Pityriasis lichenoides is a rare cutaneous entity of an unknown cause which includes different clinical presentations. A number of infectious agents have been implicated based on the clustering of …

Insecticidal activity of Vip3Aa, Vip3Ad, Vip3Ae, and Vip3Af from Bacillus thuringiensis against lepidopteran corn pests.

2012

Vip3Aa, Vip3Ad, Vip3Ae, and Vip3Af proteins from Bacillus thuringiensis were tested for their toxicity against Spodoptera frugiperda and Agrotis ipsilon. Vip3Ad was non-toxic to the two species. Vip3Ae and Vip3Af were significantly more toxic than Vip3Aa against S. frugiperda, both as protoxins and as toxins. Against A. ipsilon, Vip3Ae protoxin was more toxic than Vip3Aa and Vip3Af protoxins. Purification by metal-chelate affinity chromatography significantly affected Vip3Ae toxicity against the two insect species.