Search results for "wt"
showing 10 items of 5424 documents
Melt flow instability and vortex structures in Czochralski growth under steady magnetic fields
2002
Abstract The hydrodynamics of melt motion in the CZ-process of silicon single crystal growth in axial and axial–radial magnetic fields is studied using a physical model. The modeling liquid is eutectics InGaSn. Most attention is paid to the problem of flow stability. A multi-channel system gathering data is used to define the pattern and dynamics of velocity disturbances. Instant spatial distributions of the electric potential induced by motion in the melt are obtained. Temporal evolution of the instant distributions in vertical and horizontal planes of the working volume is under investigation.
Renewable energy for sustainable rural development: synergies and mismatches
2020
Abstract Energy transition is increasingly regarded as a promising opportunity for the economic development of rural areas. This possibility is associated with the siting and (co-)ownership of decentralized (small-scale) renewable energy facilities. The underlying productive link, however, has been taken for granted, rather than conceptually and practically cultivated. Thus, while renewable energy-based rural development has been stated as a desired by-product of energy transitions, its potential has remained largely unfulfilled. This review aims to illuminate the ambiguous interplay between renewable energy and rural development in the context of the current trajectories of the energy tran…
Stimulation of protein (collagen) synthesis in sponge cells by a cardiac myotrophin‐related molecule from Suberites domuncula
2000
The body wall of sponges (Porifera), the lowest metazoan phylum, is formed by two epithelial cell layers of exopinacocytes and endopinacocytes, both of which are associated with collagen fibrils. Here we show that a myotrophin-like polypeptide from the sponge Suberites domuncula causes the expression of collagen in cells from the same sponge in vitro. The cDNA of the sponge myotrophin was isolated; the potential open reading frame of 360 nt encodes a 120 aa long protein (Mr of 12,837). The sequence SUBDOMYOL shares high similarity with the known metazoan myotrophin sequences. The expression of SUBDOMYOL is low in single cells but high after formation of primmorph aggregates as well as in in…
Mboat7 down-regulation by hyper-insulinemia induces fat accumulation in hepatocytes.
2020
Background: Naturally occurring variation in Membrane-bound O-acyltransferase domain-containing 7 (MBOAT7), encoding for an enzyme involved in phosphatidylinositol acyl-chain remodelling, has been associated with fatty liver and hepatic disorders. Here, we examined the relationship between hepatic Mboat7 down-regulation and fat accumulation. Methods: Hepatic MBOAT7 expression was surveyed in 119 obese individuals and in experimental models. MBOAT7 was acutely silenced by antisense oligonucleotides in C57Bl/6 mice, and by CRISPR/Cas9 in HepG2 hepatocytes. Findings: In obese individuals, hepatic MBOAT7 mRNA decreased from normal liver to steatohepatitis, independently of diabetes, inflammatio…
Fostering Entrepreneurs’ Capabilities to Outline Sustainable Strategies in ‘Stunted’ SMEs through Modelling and Simulation: a Dynamic Resource Based …
The literature recognises the phenomenon of ‘dwarf’ or ‘stunted’ small and micro firms (in Italian nanismo aziendale) and that they might represent potential lost opportunities for owners and the local economy. Based on a field survey conducted by Bianchi et al. (2003) and the analysis previously developed by the authors of this paper in sketching a simple ‘insight’ model to simulate the behaviour of such firms (Bianchi & Winch, 2005), this work aims to show further research developments in the effort to better understand the business dwarfism phenomenon. A system dynamics model replicating the basic no-growth, cyclical behaviour attributed to “stunted” SMEs is firstly analysed. Alterna…
Efeitos da sustentabilidade e valor logístico nas relações entre empresas de transporte marítimo
2020
Resumen El objetivo de este trabajo fue analizar cómo la sostenibilidad (económica, social y ambiental), bajo el enfoque de triple línea base, afecta la calidad de servicio, el valor logístico y la satisfacción en empresas del sector marítimo. Se propone un modelo teórico que se contrasta con la información suministrada por una muestra de 50 responsables de empresas (transitarios, importadores y exportadores) en Panamá. La información se analiza mediante la estimación de regresión por mínimos cuadrados parciales, que permite confirmar el carácter multidimensional de la sostenibilidad y del valor logístico. Los resultados también confirman que las percepciones de los clientes sobre las inici…
Ex Vivo Retinal Explant Cultures to Study Angiogenic Responses
2012
The mouse retina has long been regarded as an easily accessible and advantageous system to investigate important questions of developmental angiogenesis. The protocol presented here profits from the suitability of the mouse retina as experimental model and describes an ex vivo culture technique of mouse retina explants that allows the quantitative assessment of angiogenic responses to pharmacological manipulations. The technique involves the extraction of the retina from the intact eye, the immediate flat mounting of the tissue on a hydrophilic membrane and the acute stimulation or inhibition of angiogenic processes of the developing vessels in their physiological context ex vivo. The numbe…
Short-Time Ocular Ischemia Induces Vascular Endothelial Dysfunction and Ganglion Cell Loss in the Pig Retina
2019
Visual impairment and blindness are often caused by retinal ischemia-reperfusion (I/R) injury. We aimed to characterize a new model of I/R in pigs, in which the intraocular pathways were not manipulated by invasive methods on the ocular system. After 12 min of ischemia followed by 20 h of reperfusion, reactivity of retinal arterioles was measured in vitro by video microscopy. Dihydroethidium (DHE) staining, qPCR, immunohistochemistry, quantification of neurons in the retinal ganglion cell layer, and histological examination was performed. Retinal arterioles of I/R-treated pigs displayed marked attenuation in response to the endothelium-dependent vasodilator, bradykinin, compared to sham-tre…
Combination of Resveratrol with omega-3 fatty acids synergize to counteract VEGF-R pathway in sick retinal pigment epithelium cells mimicking AMD
2015
Purpose The aim of this work is to assess whether RSV (Resveratrol) can act synergistically with omega-3 fatty acids to modulate VEGF (Vascular Endothelial Growth Factor) signaling pathway in order to identify a new and more effective therapy for the treatment of AMD (Age-Related Macular Degeneration). Methods In this study, undifferentiated and differentiated human retinal pigment epithelial cells (ARPE-19) were used. The cells were treated with an omega-3/RSV preparation (Resvega®), or a RSV-free formulation or RSV alone for 24 h. The expression of key proteins in VEGF signaling pathway was evaluated by Western Blotting. Results We observed that the combination omega-3/RSV preparation (Re…
The existence of FGFR1-5-HT1A receptor heterocomplexes in midbrain 5-HT neurons of the rat: relevance for neuroplasticity.
2012
The ascending midbrain 5-HT neurons to the forebrain may be dysregulated in depression and have a reduced trophic support. Within situproximity ligation assay (PLA) and supported by coimmunoprecipitation and colocation of the FGFR1 and 5-HT1A immunoreactivities in the midbrain raphe cells, evidence for the existence of FGFR1–5-HT1A receptor heterocomplexes in the dorsal and median raphe nuclei of the Sprague Dawley rat as well as in the rat medullary raphe RN33B cells has been obtained. Especially after combined FGF-2 and 8-OH-DPAT treatment, a marked and significant increase in PLA clusters was found in the RN33B cells. Similar results were reached with the FRET technique in HEK293T cells,…