0000000000055012

AUTHOR

J. Garcia De La Asuncion

showing 3 related works from this author

Oxidative damage to mitochondrial DNA and glutathione oxidation in apoptosis: studiesin vivoandin vitro

1999

Free radicals may be involved in apoptosis although this is the subject of some controversy. Furthermore, the source of free radicals in apoptotic cells is not certain. The aim of this study was to elucidate the role of oxidative stress in the induction of apoptosis in serum-deprived fibroblast cultures and in weaned lactating mammary glands as in vitro and in vivo experimental models, respectively. Oxidative damage to mtDNA is higher in apoptotic cells than in controls. Oxidized glutathione (GSSG) levels in mitochondria from lactating mammary gland are also higher in apoptosis. There is a direct relationship between mtDNA damage and the GSSG/reduced glutathione (GSH) ratio. Furthermore, wh…

DNA damageApoptosisWeaningMitochondrionmedicine.disease_causeDNA MitochondrialBiochemistryCulture Media Serum-FreeMembrane Potentialschemistry.chemical_compoundCytosolMammary Glands AnimalIn vivoGeneticsmedicineAnimalsLactationAnaerobiosisRats WistarFragmentation (cell biology)Molecular BiologyCells CulturedGlutathione DisulfideGlutathioneFibroblastsGlutathioneIn vitroPeroxidesRatsCell biologyOxidative StresschemistryApoptosisFemaleReactive Oxygen SpeciesOxidative stressDNA DamageBiotechnologyThe FASEB Journal
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Inspired supplemental oxygen reduces markers of oxidative stress during elective colon surgery.

2007

.Anaesthesia was induced with sodium thiopental andfentanyl, and maintained with sevoflurane. After inductionof anaesthesia, patients were randomly assigned 30 or80 per cent oxygen throughout surgery and for 6 hafterwards. During surgery, lungs were ventilated involume-controlled mode (10 ml/kg), whereas during the6 h afterwards respiration was spontaneous with an oxygenmask (16 l/min)

medicine.medical_specialtySupplemental oxygenchemistry.chemical_elementmedicine.disease_causeOxygenchemistry.chemical_compoundColonic DiseasesPostoperative ComplicationsColon surgeryMalondialdehydeRespirationmedicineHumansOxygen supplementationbusiness.industryOxygen Inhalation TherapyMalondialdehydeGlutathioneSurgeryOxygenOxidative StressSodium thiopentalTreatment OutcomechemistryAnesthesiaSurgerybusinessOxidative stressBiomarkersmedicine.drugThe British journal of surgery
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Late onset administration of oral antioxidants prevents age-related loss of motor co-ordination and brain mitochondrial DNA damage.

1999

We have studied the effect of aging on brain glutathione redox ratio, on brain mitochondrial DNA damage and on motor co-ordination in mice and the possible protective role of late onset administration of sulphur-containing antioxidants. Glutathione redox ratios change to a more oxidized state in whole brain with aging but the changes are much more pronounced when this ratio is measured in brain mitochondria. The levels of 8-oxo-7,8-dihydro-2 '-deoxyguanosine in mitochondrial DNA are much higher in the brain of old animals than in those of young ones. Late onset oral administration of sulphur-containing antioxidants partially prevents oxidation of mitochondrial glutathione and DNA. There is …

Malemedicine.medical_specialtyMitochondrial DNAAgingAdministration OralLate onsetMice Inbred StrainsBiologyMotor Activitymedicine.disease_causeBiochemistryRedoxDNA MitochondrialAntioxidantsDrug Administration Schedulechemistry.chemical_compoundMiceOral administrationInternal medicineAge relatedmedicineAnimalsPostural BalanceAlanineBrainDeoxyguanosineGeneral MedicineGlutathioneMolecular biologyGlutathioneThiazolesEndocrinologychemistry8-Hydroxy-2'-DeoxyguanosineOxidation-ReductionOxidative stressDNASulfurDNA DamageFree radical research
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