0000000000082477
AUTHOR
José Reig-armiñana
showing 15 related works from this author
Molecular and morphological diversity ofTrebouxiamicroalgae in sphaerothallioidCircinariaspp. lichens1
2018
Three vagrant (Circinaria hispida, Circinaria gyrosa, and Circinaria sp. 'paramerae') and one crustose (semi-vagrant, Circinaria sp. 'oromediterranea') lichens growing in very continental areas in the Iberian Peninsula were selected to study the phycobiont diversity. Mycobiont identification was checked using nrITS DNA barcoding: Circinaria sp. 'oromediterranea' and Circinaria sp. 'paramerae' formed a new clade. Phycobiont diversity was analyzed in 50 thalli of Circinaria spp. using nrITS DNA and LSU rDNA, with microalgae coexistence being found in all the species analyzed by Sanger sequencing. The survey of phycobiont diversity showed up to four different Trebouxia spp. as the primary phyc…
Ozone-induced reductions in below-ground biomass: an anatomical approach in potato
2010
[EN] Potato plants were grown in open-top chambers under three ozone concentrations during two complete cropping seasons (93 and 77 d in 2004 and 2005, respectively). The effects of chronic exposure to ozone on leaf anatomy, cell ultrastructure and crop yield were studied. Severe cell damage was found, even at ambient ozone levels, mainly affecting the spongy parenchyma and areas near the stomata. Damage to the cell wall caused loss of cell contact, and loss of turgor pressure due to tonoplast disintegration, contributed to cell collapse. Phloem sieve plates were obstructed by callose accumulation, and damaged mesophyll cells increased their starch stores. Tuber yield fell sharply (24–44%),…
Inhibition of NO Biosynthetic Activities during Rehydration of Ramalina farinacea Lichen Thalli Provokes Increases in Lipid Peroxidation
2019
Lichens are poikilohydrous symbiotic associations between a fungus, photosynthetic partners, and bacteria. They are tolerant to repeated desiccation/rehydration cycles and adapted to anhydrobiosis. Nitric oxide (NO) is a keystone for stress tolerance of lichens
Morphology, anatomy and germination response of heteromorphic achenes of Anthemis chrysantha J. Gay (Asteraceae), a critically endangered species
2011
[EN] This study demonstrates that Anthemis chrysantha, a 'Critically Endangered' annual plant, produces two morphs of achenes: white and dark achenes, which differ in size, mass, anatomy and germination behaviour. Fresh white achenes germinated at all temperatures assayed from 10 to 25 degrees C in both continuous darkness and 12-h photoperiod, ranging between 24% at 25 degrees C in darkness and 89% at 12/20 degrees C in light, whereas fresh dark achenes did not germinate under any temperature or light conditions. To identify differences in dormancy type between the two morphs, germination of dry-stored achenes, and achenes stratified at 5 or 25 degrees C for 2 months were tested in both da…
Ultrastructural and histochemical analysis reveals ethylene-induced responses underlying reduced peel collapse in detached citrus fruit
2010
Fruits from many citrus cultivars develop depressed areas in the flavedo (outer part of the peel) and albedo (inner part) following detachment. Although ultrastructural analysis may provide important information about multiple plant responses to stresses and external stimuli at the cell and tissue levels, and despite the proved efficacy of ethylene in reducing peel damage in citrus fruit, cytological responses of this horticultural crop to protective ethylene concentrations have not yet been reported. We show that applying high ethylene levels (2 mu L L(-1) for 14 days) causes sublethal stress as it favored the alteration of cuticle, vacuole, middle lamella and primary wall, especially in t…
The organic air pollutant cumene hydroperoxide interferes with NOantioxidant role in rehydrating lichen
2013
Organic pollutants effects on lichens have not been addressed. Rehydration is critical for lichens, a burst of free radicals involving NO occurs. Repeated dehydrations with organic pollutants could increase oxidative damage. Our aim is to learn the effects of cumene hydroperoxide (CP) during lichen rehydration using Ramalina farinacea (L.) Ach., its photobiont Trebouxia spp. and Asterochloris erici. Confocal imaging shows intracellular ROS and NO production within myco and phycobionts, being the chloroplast the main source of free radicals. CP increases ROS, NO and lipid peroxidation and reduces chlorophyll autofluorescence, although photosynthesis remains unaffected. Concomitant NO inhibit…
Molecular phylogeny and ultrastructure of the lichen microalga Asterochloris mediterranea sp. nov. from Mediterranean and Canary Islands ecosystems
2015
The microalgae of the genus Asterochloris are the preferential phycobionts in Cladonia, Lepraria and Stereocaulon lichens. Recent studies have highlighted the hidden diversity of the genus, even though phycobionts hosting Cladonia spp. in Mediterranean and Canarian ecosystems have been poorly explored. Phylogenetic analyses were made by concatenation of the sequences obtained with a plastid -LSU rDNA- and two nuclear -ITS rDNA and actin- molecular markers of the phycobionts living in several populations of Cladonia convoluta-C. foliacea complex, C. rangiformis and C. cervicornis species widely distributed in these areas in a great variety of substrata and habitats. A new strongly supported …
Effects of ozone on the foliar histology of the mastic plant (Pistacia lentiscus L.)
2004
[EN] An open-top chamber study was conducted to investigate the tissue and cellular-level foliar effects of ozone (O-3) on a Mediterranean evergreen species, the mastic plant (Pistacia lentiscus L.). Plants were exposed at three different O-3 levels, and leaf samples were collected periodically from the beginning of the exposure. Although no visible foliar injury was evident, alterations of the plastids and vacuoles in the mesophyll were observed. Senescence processes were accelerated with an anomalous stacking of tannin vacuoles, and a reduction in the size and number of the chloroplasts. Overall, most of the modifications induced by O-3 were consistent with previously reported observation…
Physiological,anatomical and biomass partitioning responses to ozone in the Mediterranean endemic plant Lamottea dianae
2011
Ozone effects on the perennial forb Lamottea dianae were studied in an open-top chamber experiment. Ozone was found to induce reductions in CO 2 assimilation and water use efficiency in the leaves of this species. These reductions were mainly related to a decline in the in vivo CO 2 fixation capacity of Rubisco (V c,max), rather than to stomatal limitations or photoinhibitory damage (F v:F m). In addition to chloroplast degeneration, other observed effects were callose accumulation, formation of pectinaceous wart-like cell wall exudates and phloem alterations. Moreover, ozone exposure significantly reduced root dry biomass. The possible relevance of these adverse effects for Mediterranean f…
Two Trebouxia algae with different physiological performances are ever-present in lichen thalli of Ramalina farinacea. Coexistence versus Competition?
2010
This study was funded by the Spanish Ministry of Education and Science (CGL2006-12917-C02-01/02), the Spanish Ministry of Science and Innovation (CGL2009-13429-C02-01/02), the AECID (PCI_A/024755/09) and the Generalitat Valenciana (PROMETEO 174/2008 GVA). We are grateful to Dr J. Gimeno-Romeu (University of California, Davis, USA) and to Dr P. J. G. de Nova (IREC, Ciudad Real, Spain), who were the first to isolate DNA from Ramalina farinacea thalli in our group. Wendy Ran revised the manuscript in English.
Effects of elevated ozone on physiological, anatomical and ultrastructural characteristics of four common urban tree species in China
2016
Fast urbanization has led to ozone (O3) being the main pollutant in summer in most of China. To assess future ground-level O3 effects on the service of urban greening species and clarify the underlying mechanism of O3 damage, four common urban greening species, Ailanthus altissima (AA), Fraxinus chinensis(FC), Platanus orientalis (PO) and Robinia pseudoacacia (RP) were exposed to non-filtered air (NF) and to elevated O3 (E-O3) in open-top chambers. E-O3 induced visible injury in all species as well as microscopic alterations such as collapse of the palisade parenchyma cells, callose accumulation, or chloroplast and mitochondrial accelerated senescence. E-O3 significantly reduced light-satur…
Epicuticular wax content and morphology as related to ethylene and storage perfomance of 'Navelate' orange fruit
2010
8 pages, 6 figures, 1 table. -- Available online 15 September 2009.
Different strategies to achieve Pb-tolerance by the two Trebouxia algae coexisting in the lichen Ramalina farinacea.
2012
Lichen thalli are permeable to airborne substances, including heavy metals, which are harmful to cell metabolism. Ramalina farinacea shows a moderate tolerance to Pb. This lichen comprises two Trebouxia phycobionts, provisionally referred to as TR1 and TR9, with distinct physiological responses to acute oxidative stress. Thus, there is a more severe decay in photosynthesis and photosynthetic pigments in TR1 than in TR9. Similarly, under oxidative stress, antioxidant enzymes and HSP70 protein decrease in TR1 but increase in TR9. Since Pb toxicity is associated with increased ROS formation, we hypothesized greater Pb tolerance in this phycobiont. Accordingly, the aim of the present study was …
Fungal-associated NO is involved in the regulation of oxidative stress during rehydration in lichen symbiosis
2010
[EN] Background Reactive oxygen species (ROS) are normally produced in respiratory and photosynthetic electron chains and their production is enhanced during desiccation/rehydration. Nitric oxide (NO) is a ubiquitous and multifaceted molecule involved in cell signaling and abiotic stress. Lichens are poikilohydrous organisms that can survive continuous cycles of desiccation and rehydration. Although the production of ROS and NO was recently demonstrated during lichen rehydration, the functions of these compounds are unknown. The aim of this study was to analyze the role of NO during rehydration of the lichen Ramalina farinacea (L.) Ach., its isolated photobiont partner Trebouxia sp. and Ast…
Estudio anatómico-histológico de las flores del endemismo Lamottea diania (Asteraceae) y de los efectos del ozono troposférico sobre su desarrollo.
2016
"Estudio anatómico-histológico de las flores del endemismo Lamottea diania (Asteraceae) y de los efectos del ozono troposférico sobre su desarrollo". Lilloa 53 (2). Plantas del endemismo Lamottea diania se expusieron en cámaras OTC (Open Top Chambers) a aire ambiente filtrado y a aire ambiente no filtrado más 30 ppb de ozono para observar el efecto de este contaminante sobre el desarrollo de sus flores, particularmente sobre su androceo. Hemos comprobado, mediante estudios de microscopía óptica y electrónica, que el ozono afecta el proceso de desarrollo y maduración de los estambres y del polen. Las anteras se ven afectadas, en unos casos al abortar algunos estambres en su desarrollo y, en …