0000000000082865
AUTHOR
Yevgeny Beiderman
Coherent Microscopy for 3-D Movement Monitoring and Super-Resolved Imaging
In this chapter we present three types of microscopy-related configurations while the first one is used for 3-D movement monitoring of the inspected samples, the second one is used for super-resolved 3-D imaging, and the last one presents an overview digital holographic microscopy applications. The first configuration is based on temporal tracking of secondary reflected speckles when imaged by properly defocused optics. We validate the proposed scheme by using it to monitor 3-D spontaneous contraction of rat’s cardiac muscle cells while allowing nanometric tracking accuracy without interferometric recording. The second configuration includes projection of temporally varying speckle patterns…
Photonic non-contact estimation of blood lactate level
The ability to measure the blood lactate level in a non-invasive, non-contact manner is very appealing to the sports industry as well as the home care field. That is mainly because this substance level is an imperative parameter in the course of devolving a personal workout programs. Moreover, the blood lactate level is also a pivotal means in estimation of muscles' performance capability. In this manuscript we propose an optical non-contact approach to estimate the concentration level of this parameter. Firstly, we introduce the connection between the physiological muscle tremor and the lactate blood levels. Secondly, we suggest a photonic optical method to estimate the physiological tremo…
Demonstration of remote optical measurement configuration that correlates to glucose concentration in blood
An optical approach allowing the extraction and the separation of remote vibration sources has recently been proposed. The approach has also been applied for medical related applications as blood pressure and heart beats monitoring. In this paper we demonstrate its capability to monitor glucose concentration in blood stream. The technique is based on the tracking of temporal changes of reflected secondary speckle produced in human skin (wrist) when being illuminated by a laser beam. A temporal change in skin’s vibration profile generated due to blood pulsation is analyzed for estimating the glucose concentration. Experimental tests that were carried out in order to verify the proposed appro…
Three-Dimensional Mapping and Ranging of Objects Using Speckle Pattern Analysis
In this chapter, we present two novel approaches for 3-D object shape measurement and range estimation based on digital image processing of speckle patterns. In the first one, 3-D mapping and range measurement are retrieved by projecting, through a ground glass diffuser, random speckle patterns on the object or on the camera for a transmissive and reflective configuration, respectively. Thus, the camera sensor records in time sequence different speckle patterns at different distances, and by using correlation operation between them, it is possible to achieve 3-D mapping and range finding. In the second one, the 3-D mapping and ranging are performed by sensing the visibility associated with …
A microscope configuration for nanometer 3-D movement monitoring accuracy.
In this paper we present a new microscopy configuration based upon temporal tracking of a secondary reflected speckle by imaging the speckle through properly defocused optics. The configuration is used to monitor three-dimensional (3-D) spontaneous contraction of rat cardiac muscle cells while achieving nanometer tracking accuracy at a rate of 30 frames per second (fps) without using interferometric recording. Estimation of the change in the optical path of accuracy of 50 nm in the transverse direction and of 200 nm in the axial direction was achieved.
Speckle based configuration for simultaneousin vitroinspection of mechanical contractions of cardiac myocyte cells
In this manuscript we propose optical lensless configuration for a remote non-contact measuring of mechanical contractions of vast number of cardiac myocytes. All the myocytes were taken from rats, and the measurements were done in an in vitro mode. The optical method is based on temporal analysis of secondary reflected speckle patterns generated in lensless microscope configuration. The processing involves analyzing the movement and the change in the statistics of the generated secondary speckle patterns that are created on top of the cell culture when it is illuminated by a spot of laser beam. The main advantage of the proposed system is the ability to measure many cells simultaneously (a…
Optical remote sensor for alcohol concentration in blood
In this paper we incorporate recently developed novel optical approach for extraction of remote vibration sources to estimate the alcohol concentration in blood stream.
Optical sensor for remote estimation of alcohol concentration in blood stream
Abstract The purpose of this manuscript is to validate our recently developed novel optical approach for extraction of remote vibration sources as a successful technique to estimate the alcohol concentration in blood stream. This technique is based on the tracking of temporal changes of reflected secondary speckle patterns produced in human skin when being illuminated by a laser beam. Since the skin’s vibrations profile is changed due to the alcohol in the blood stream, the extraction of the vibration profile can be translated into the corresponding alcohol concentration values by means of defining several parameters acting as indicators for the presence of alcohol in the blood stream. We h…
Simultaneous remote extraction of multiple speech sources and heart beats from secondary speckles pattern
The ability of dynamic extraction of remote sounds is very appealing. In this manuscript we propose an optical approach allowing the extraction and the separation of remote sound sources. The approach is very modular and it does not apply any constraints regarding the relative position of the sound sources and the detection device. The optical setup doing the detection is very simple and versatile. The principle is to observe the movement of the secondary speckle patterns that are generated on top of the target when it is illuminated by a spot of laser beam. Proper adaption of the imaging optics allows following the temporal trajectories of those speckles and extracting the sound signals ou…
Rounding noise effects’ reduction for estimated movement of speckle patterns
The problem of resolution enhancement for speckle patterns analysis-based movement estimation is considered. In our previous publications we showed that this movement represents the corresponding tilt vibrations of the illuminated object and can be measured as a relative spatial shift between time adjacent images of the speckle pattern. In this paper we show how to overcome the resolution limitation obtained when using an optical sensor available in an optical mouse and which measures the Cartesian coordinates of the shift as an integer number of pixels. To overcome such a resolution limitation, it is proposed here to use simultaneous measurements from the same illuminated spot by a few cam…
Improved noncontact optical sensor for detection of glucose concentration and indication of dehydration level.
The ability to extract different bio-medical parameters from one single wristwatch device can be very applicable. The wearable device that is presented in this paper is based on two optical approaches. The first is the extraction and separation of remote vibration sources and the second is the rotation of linearly polarized light by certain materials exposed to magnetic fields. The technique is based on tracking of temporal changes of reflected secondary speckles produced in the wrist when being illuminated by a laser beam. Change in skin’s temporal vibration profile together with change in the magnetic medium that is generated by time varied glucose concentration caused these temporal chan…
Three-dimensional mapping and range measurement by means of projected speckle patterns.
We present a novel approach for three-dimensional (3D) measurements that includes the projection of coherent light through ground glass. Such a projection generates random speckle patterns on the object or on the camera, depending if the configuration is transmissive or reflective. In both cases the spatially random patterns are seen by the sensor. Different spatially random patterns are generated at different planes. The patterns are highly random and not correlated. This low correlation between different patterns is used for both 3D mapping of objects and range finding.
Noncontact speckle-based optical sensor for detection of glucose concentration using magneto-optic effect
We experimentally verify a speckle-based technique for noncontact measurement of glucose concentration in the bloodstream. The final device is intended to be a single wristwatch-style device containing a laser, a camera, and an alternating current (ac) electromagnet generated by a solenoid. The experiments presented are performed in vitro as proof of the concept. When a glucose substance is inserted into a solenoid generating an ac magnetic field, it exhibits Faraday rotation, which affects the temporal changes of the secondary speckle pattern distributions. The temporal frequency resulting from the ac magnetic field was found to have a lock-in amplification role, which increased the observ…
Remote estimation of blood pulse pressure via temporal tracking of reflected secondary speckles pattern
We present a novel technique for remote noncontact blood pulse pressure measurement. It is based on tracking both temporal and amplitude changes of reflected secondary speckle produced in human skin when illuminated by a laser beam. The implemented technique extracts the difference between the systolic and the diastolic blood pressure. Experimental results are presented showing good agreement when compared with conventional measurement methods.
Optical technique for classification, recognition and identification of obscured objects
Abstract The capability to classify, recognize and to identify objects from spatially low resolution images has high significance in security related applications especially in a case that recognition of camouflaged object is required. In this paper we present a novel approach in which the scenery containing obscured objects which we wish to classify, recognize or identify is illuminated by spatially coherent beam (e.g. laser) and therefore secondary speckles pattern is reflected from the objects. By special image processing algorithm developed for this research and which is basically based upon temporal tracking of the random speckle pattern one may extract the temporal signature of the ob…
New method for remote and repeatable monitoring of intraocular pressure variations
We present initial steps toward a new measurement device enabling high-precision, noncontact remote and repeatable monitoring of intraocular pressure (IOP)-based on an innovative measurement principle. Using only a camera and a laser source, the device measures IOP by tracking the secondary speckle pattern trajectories produced by the reflection of an illuminating laser beam from the iris or the sclera. The device was tested on rabbit eyes using two different methods to modify IOP: via an infusion bag and via mechanical pressure. In both cases, the eyes were stimulated with increasing and decreasing ramps of the IOP. As IOP variations changed the speckle distributions reflected back from th…
Robotic Platform for Automated Search and Rescue Missions of Humans
We present a novel type of model incorporating a special remote life signals sensing optical system on top of a controllable robotic platform. The remote sensing system consists of a laser and a camera. By properly adapting our optics and by applying a proper image processing algorithm we can sense within the field of view, illuminated by the laser and imaged by the camera, the heartbeats and the blood pulse pressure of subjects (even several simultaneously). The task is to use the developed robotic system for search and rescue mission such as saving survivals from a fire.
Remote optical sensor of blood coagulation, oximetry and dehydration
An optical approach for remote extraction of vibrations has recently been proposed. We demonstrate the method's ability to continuously monitor three biomedical indicators: blood oximetry, blood coagulation and dehydration of the body.
Speckle-based configuration for simultaneous in vitro inspection of mechanical contractions of cardiac myocyte cells.
An optical lensless configuration for a remote noncontact measuring of mechanical contractions of a vast number of cardiac myocytes is proposed. All the myocytes were taken from rats, and the measurements were done in an in vitro mode. The optical method is based on temporal analysis of secondary reflected speckle patterns generated in lensless microscope configuration. The processing involves analyzing the movement and the change in the statistics of the secondary speckle patterns that are created on top of the cell culture when it is illuminated by a spot of laser beam. The main advantage of the proposed system is the ability to measure many cells simultaneously (∼1000 cells) and to extra…
Projection of speckle patterns for 3D sensing
In this communication we present the use of projected speckle patterns coming from a phase random mask for sensing depths and thicknesses. The sensing is based on the change of the speckle pattern with propagation and the lack of correlation between speckle patterns recorded at different depths or lateral locations. The principle is used for mapping thickness of transparent media, for depth ranging and for 3D mapping of diffuse objects.