Quantitative Bestimmung von Sterigmatocystin in verschimmelten pflanzlichen Nahrungsmitteln

Ein Verfahren fur die quantitative Bestimmung von Sterigmatocystin in pflanzlichen Nahrungsmitteln durch zweidimensionale DC-Trennung eines Extraktes und Fluorescenzintensitatsmessung der mit AlCl3 behandelten DC-Platte wird beschrieben. Durch die hier vorgeschlagene Auftrageweise auf der DC-Platte ist ein rasches Identifizieren und Auswerten von Probe- und Eichflecken moglich. Als Extraktionsmittel hat sich Acetonitril-KCl-Losung am besten bewahrt. Versuche mit zugesetztem Toxin zeigten Verluste von weniger als 5 %.

Quantitative Bestimmung von Trichothecenen HT-2 Toxin in verschimmelten pflanzlichen Nahrungsmitteln

Es wird ein Verfahren zur quantitativen Bestimmung von HT-2 Toxin in verschimmeltem Reis, Mais, Weizen, Hafer, Roggen bzw. Erbsen beschrieben. Extrahiert wurde mit einer Mischung von 9 Teilen (Volumen) Acetonitril und einem Teil wasriger 4%iger KCl-Losung. Die Extrakte musten teilweise chromatographisch vorgereinigt werden. Die terminale Bestimmung erfolgte nach zweidimensionaler dunnschichtchromatographischer Trennung durch Fluorescenzintensitatsmessung der mit H2SO4 behandelten DC-Platten. Versuche mit zugesetztem HT-2 Toxin ergaben eine Wiederfindung von 88% (Variationskoeffizient 7).

High-performance liquid chromatography of trichothecenes

Progressive femoral cortical and cancellous bone density loss after uncemented tapered-design stem fixation

Background Aseptic implant loosening and periprosthetic bone loss are major problems after total hip arthroplasty (THA). We present an in vivo method of computed tomography (CT) assisted osteodensitometry after THA that differentiates between cortical and cancellous bone density (BD) and area around the femoral component.Method Cortical and cancellous periprosthetic femoral BD (mg CaHA/mL), area (mm2) and contact area between the prothesis and cortical bone were determined prospectively in 31 patients 10 days, 1 year, and 6 years after uncemented THA (mean age at implantation: 55 years) using CT-osteodensitometry.Results 6 years postoperatively, cancellous BD had decreased by as much as 41%…

Modes of periacetabular load transfer to cortical and cancellous bone after cemented versus uncemented total hip arthroplasty: A prospective study using computed tomography-assisted osteodensitometry

Stress-shielding and periprosthetic bone loss after total hip arthroplasty (THA) may be clinically relevant for high-demand patients. Analysis of cortical and cancellous bone density (BD) changes in vivo after THA is of interest to basic science researchers and joint reconstruction surgeons. An insufficient periprosthetic bone stock may predispose to migration, early mechanical failure, and major problems in revision surgery. We used computed tomography (CT)-assisted osteodensitometry in two prospectively analyzed cohorts after cemented (n = 21) versus noncemented (n = 23) cup fixation. Periacetabular BD (mgCaHa/mL) was determined in five CT scans cranial and five CT scans at the level of t…

High-performance liquid chromatography of the mycotoxin sterigmatocystin and its application to the analysis of mouldy rice for sterigmatocystin.

HPLC: a tool for the analysis of T-2 toxin and HT-2 toxin in cereals.

An analytical procedure for the determination of trichothecenes in various cereals is described. HPLC was performed with a reversed-phase (C18) column eluted with methanol:water (60:40, v/v). Compounds were detected with a refractive index detector. The elution patterns of free and contaminated samples were compared. The recovery of added T-2 toxin (2 and 5 micrograms/g) in rye and wheat was approximately 80%. The application of this method allows for combined use with other sensitive methods such as mass spectrometry and gas chromatography. The described method is operationally simple, relatively inexpensive, and requires no derivatization.

Quantitative computed tomography-assisted osteodensitometry of the pelvis after press-fit cup fixation: a prospective ten-year follow-up.

Background: As a follow-up of a previously reported three-year study, we analyzed the periprosthetic acetabular cortical and cancellous bone density changes at ten years after implantation of a press-fit cup. Methods: Prospective clinical, radiographic, and quantitative computed tomography examinations were performed within ten days and at mean periods of one, three, and ten years after total hip arthroplasty with a press-fit cup, a femoral stem with a tapered design, and alumina-alumina pairing. Periacetabular cortical and cancellous bone density (mg CaHA/mL) in the cranial, ventral, and dorsal regions about the cup were measured for twenty-four hips in vivo. Results: All acetabular cups s…

Periacetabular Cortical and Cancellous Bone Mineral Density Loss After Press-Fit Cup Fixation

The impact of total hip arthroplasty on strain adaptive bone remodeling has been extensively analyzed by dual-energy x-ray absorptiometry. In this study, we present a prospective computed tomography-assisted study of periacetabular cortical and cancellous bone mineral density (in milligrams of calcium hydroxyapatite [CaHA] per milliliter, or mgCaHA/mL) changes 10 days and 1, 3, and 7 years after press-fit cup implantation for 38 hips in vivo. Cancellous bone mineral density decreased by O -63% ventral and O -85% dorsal to the cup; cortical bone mineral density, by O -22% ventral and O -18% dorsal to the cup. The presented periacetabular strain adaptive bone mineral density data are the most…

Bestimmung von T-2-Toxin in pflanzlichen Nahrungsmitteln

Ein Verfahren zur quantitativen Bestimmung von T-2-Toxin in verschimmeltem Reis bzw. Mais durch Extraktion, dunnschicht-chromatographische Trennung und Fluorescenzintensitatsmessung der mit H2SO4 behandelten DC-Platte wird vorgestellt. Fluorescenzabsorptions- und Fluorescenzemissionsspektrum werden angegeben. Die Abhangigkeit der Mycotoxinkonzentration von der Fluorescenzintensitat wird durch eine Gleichung beschrieben. Die analytische Detektion von T-2-Toxin last sich durch ein 4-(p-Nitrobenzyl)pyridin-Derivat und dessen Absorptionsspektrum bestatigen. Versuche mit zugesetztem T-2-Toxin zeigten einen Analysenfehler von weniger als 10%.