0000000000226295
AUTHOR
P. Braun
Increase in transmitted resistance to non-nucleoside reverse transcriptase inhibitors among newly diagnosed HIV-1 infections in Europe
Background: One out of ten newly diagnosed patients in Europe was infected with a virus carrying a drug resistant mutation. We analysed the patterns over time for transmitted drug resistance mutations (TDRM) using data from the European Spread program.Methods: Clinical, epidemiological and virological data from 4317 patients newly diagnosed with HIV-1 infection between 2002 and 2007 were analysed. Patients were enrolled using a pre-defined sampling strategy.Results: The overall prevalence of TDRM in this period was 8.9% (95% CI: 8.1-9.8). Interestingly, significant changes over time in TDRM caused by the different drug classes were found. Whereas nucleoside resistance mutations remained con…
The Influence of Brassinosteroid, a Growth-promoting Steroidal Lactone, on Development and CO2-fixation Capacity of Intact Wheat and Mustard Seedlings
In 1970, Mitchell et al. isolated a lipoidal complex from the pollen of rape (Brassica napus L.). This complex, called “Brassins” was found to have partly powerful growth-promoting properties. The novel growth response of young bean plants in the second internode assay (Worley, Mitchell 1971; Mitchell, Gregory 1972) and other physiological changes following Brassin-treatment have been studied under several aspects (Krizek, Worley 1981; Gregory 1981).
Transmission of HIV Drug Resistance and the Predicted Effect on Current First-line Regimens in Europe
Transmitted human immunodeficiency virus drug resistance in Europe is stable at around 8%. The impact of baseline mutation patterns on susceptibility to antiretroviral drugs should be addressed using clinical guidelines. The impact on baseline susceptibility is largest for nonnucleoside reverse transcriptase inhibitors.
The influence of brassinosteroid on growth and parameters of photosynthesis of wheat and mustard plants.
The growth response of wheat (Triticum aest. L.) and mustard seedlings (Sinapis alba L.) treated with 10(-6) mol · l(-1) brassinosteroid (BR) foliar spray was measured. BR-treatment resulted in a general promotion of plant growth. We found the accumulation of photosynthates to be stimulated in the treated plants, as indicated by enhanced fresh and dry weights of leaves and shoots. BR also promoted the synthesis of soluble proteins and soluble reducing sugars, whereas the chlorophyll content was hardly affected. CO(2)-fixation in vivo as well as the (in vitro) RubPC-ase activity of BR-treated leaves were enhanced. In the developing wheat leaves we detected no difference in the ratio fraction…
Ribulose-1,5-bisphosphate Carboxylase-Oxygenase: New Aspects Respective the pH-Dependance of the Carboxylation Reaction
The investigation was directed towards the effects of reaction conditions, substrates and pH on the carboxylation reaction of ribulose-1 ,5-bisphosphate carboxylase-oxygenase in the crude enzyme extracts from several plants. Optimal substrate concentrations (HCO3 - and RubP) were determined. The highest carboxylase activity was attained with Tris/HCl buffer. The pH activity profile was quite sharp with an optimum at pH 7.8. Purified and crystallized carboxylase yielded a broad optimum curve under the same reaction conditions
Treatment-associated polymorphisms in protease are significantly associated with higher viral load and lower CD4 count in newly diagnosed drug-naive HIV-1 infected patients
Background: The effect of drug resistance transmission on disease progression in the newly infected patient is not well understood. Major drug resistance mutations severely impair viral fitness in a drug free environment, and therefore expected to revert quickly. Compensatory mutations, often already polymorphic in wild-type viruses, do not tend to revert after transmission. While compensatory mutations increase fitness during treatment, their presence may also modulate viral fitness and virulence in absence of therapy and major resistance mutations. We previously designed a modeling technique that quantifies genotypic footprints of in vivo treatment selective pressure, including both drug …
Limited cross-border infections in patients newly diagnosed with HIV in Europe
Background: International travel plays a role in the spread of HIV-1 across Europe. It is, however, not known whether international travel is more important for spread of the epidemic as compared to endogenous infections within single countries. In this study, phylogenetic associations among HIV of newly diagnosed patients were determined across Europe.Results: Data came from the SPREAD programme which collects samples of newly diagnosed patients that are representative for national HIV epidemics. 4260 pol sequences from 25 European countries and Israel collected in 2002-2007 were included.We identified 457 clusters including 1330 persons (31.2% of all patients). The cluster size ranged bet…