0000000000244110
AUTHOR
M. P. Dierich
Herpes simplex virus type 1 and 2 in the adrenal glands: Replication and histopathology
The adrenal glands were shown to be the most severely infected organs in the early phase of HSV-1 infections (up to 10 days p.i.) after i.p. infections in mice. Virus could be isolated from the adrenal glands as early as one hour after infection with pathogenic and apathogenic strains. Infection of the adrenal glands is a result of viremia. The content of HSV-1 (5 strains) was much higher in the adrenals than in spleen and liver. It peaked at 3-4 days p.i. compared to 1-2 days in spleen and liver. Only strain 17 syn+ produced low tissue titres in the adrenal glands. Morphologic alterations by HSV-1 infections commenced with distinct foci 2 days after infection in the zona fasciculata, detec…
Factors influencing the interaction of herpes simplex virus glycoprotein C with the third component of complement.
The factors influencing the interaction of herpes simplex virus (HSV) glycoprotein C (gC) with the third component of complement (C3) were investigated in this study. The ability of gC of HSV type 1 (gC-1) to bind to the C3b fragment of C3 was found to be influenced by cell specific processing of gC-1 in a different manner, binding being remarkably enhanced in some cell lines following removal of sialic acid residues. Testing several intertypic recombinants of HSV we found that only strains expressing gC-1 exhibited binding to C3b, even though their genome consisted mainly of HSV-2 sequences in some recombinants. Expression of type-2 glycoproteins gB, gD, gE, gG, gH, and gI did not alter th…
Conditions for the Enhancing Effect of Protease Inhibitors on the Concanavalin A Induced Thymidine Response of Murine Lymphocytes
Incorporation of [<sup>3</sup>H]-thymidine – [<sup>3</sup>H]-TdR – into concanavalin A (Con A) stimulated murine splenocytes and thymocytes was found to be enhanced by addition of certain concentrations of phenyl-methyl-sulfonylfluoride (PMSF), di-isopropylfluorophosphate (DFP), N-α-tosyl-<i>L</i>-lysyl-<i>L</i>-chloromethylketone (TLCK), and soybean trypsin inhibitor (SBTI). No enhancement could be observed when mononuclear cells of the peripheral blood were used, and a medium enhancement when thymocytes were applied. Furthermore, no enhancing effect of the protease inhibitors (PI) on the Con A response of murine splenocytes could be observed…
Capacity of Gentamicin-Treated Pseudomonas aeruginosa to Induce Granulocyte Locomotion
In protection against an invading parasite the host’s granulocytes play an essential role. To exert their defense the phagocytes have to be attracted to the site of parasite invasion. We have shown that Pseudomonas aeruginosa ATCC 27853 and other clinical isolates of this species can induce granulocyte migration, either directly or via generation of chemotactic activity in serum [1].
K Cell Activity of Normal and Chronic Lymphocytic Leukaemia Lymphocytes: Association with Lymphocytes bearing Receptors for Human C3b
Surface properties of effector cells in antibody-dependent cell mediated cytotoxicity (ADCC) are at present under intensive investigation. Among these cells a lymphocyte population (K cells) has been described with receptors for the Fc portion of IgG (4), receptors for complement (11) and lacking receptors for sheep red blood cells (SRBC) and surface immunoglobulin (13). The purpose of the present study was to investigate more thoroughly the relationship between K cell activity in ADCC and cells bearing receptors for C3b and C3d.