Vascular atherosclerotic disease: evaluation of the whole-blood filterability and red cell membrane microrheological pattern after intravenous administration of a single dose of pentoxifylline.

SummaryA study was carried out in 10 patients with vascular atherosclerotic disease to investigate the effects of a single dose of 300 mg pentoxifylline, given by intravenous infusion over 30 minutes, on various macrorheological and nzicrorheological parameters. Measurements were made at baseline, at the end of the intravenous infusion and again 30 minutes later of whole-blood filterability, erythrocyte membrane fluidity (using pyrene as probe) and transverse fluidity gradient (using fluorescent fatty acids as probes). The results showed that there was a significant variation in whole-blood filterability and the red cell membrane fluidity gradient after pentoxifylline. No difference, howeve…

Red cell Ca2+ content (total and cytosolic) and erythrocyte membrane fluidity in several clinical conditions

Diabetic retinopathy: Monitoring of the macro and microrheological parameters after oral administration of a single dose of calcium dobesilate (Doxium®)

Red Cell Filterability and Erythrocyte Membrane Microviscosity during Ticlopidine Treatment

The effects of 10 days' treatment with 500 mg/day ticlopidine on the filterability of red blood cells in suspension and on the microviscosity of the red blood cell membrane was evaluated in 11 patients with vascular atherosclerosis. The results show ticlopidine to be effective in influencing the rheological measures of red cell filterability and membrane microviscosity: filterability was increased and microviscosity was decreased. Ticlopidine also decreased the extent of fluorescence polarization. These results are discussed regarding possible mechanisms of action of ticlopidine.

Red Cell Membrane Protein Lateral Mobility in Diabetes Mellitus

In a group of 24 diabetics subdivided for type, we evaluated the red cell membrane protein lateral mobility marking intact red cells with pyrene-3-maleimide (3-PM) and calculating the dimer to monomer fluorescence intensity ratio (Iex/Im). The same fluorescent parameter was determined in a group of 13 normal controls. From the obtained data, it is evident that the red cell membrane protein lateral mobility clearly discriminates normals from diabetics of type 1 and 2. In normals and in diabetics of type 1 and 2 no relationship is present between this fluorescent determinant and the glycometabolic parameters (FBGL and HbA1c); considering all the diabetics, a negative relationship is evident b…

Macro and microrheological determinants in chronic renal failure

Platelet membrane fluidity and platelet membrane lipid pattern in several clinical conditions

Effect of nimodipine on rheologic parameters in patients with chronic cerebrovascular disease

Abstract Sixteen patients with chronic cerebrovascular disease were treated with a monotherapeutic regimen of nimodipine 30 mg orally three times a day. At baseline and after 45 and 90 days of therapy, the patients' whole blood filterability, erythrocyte membrane fluidity, red blood cell membrane protein lateral mobility, and red blood cell Ca 2+ content (total and cytosolic) were evaluated. After 45 days, an increase in whole blood filterability and a decrease in the cytosolic red cell Ca 2+ content was seen. At the end of treatment, whole blood filterability, erythrocyte membrane fluidity, and red cell membrane protein lateral mobility had increased, compared with baseline values, while t…

Diabetes mellitus: mean erythrocyte aggregation, glycometabolic pattern, red cell Ca2+ content, and erythrocyte membrane dynamic properties.

Red Cell Membrane Protein Lateral Mobility in Vascular Atherosclerotic Disease: Preliminary Report

Effect of mesoglycan on macrorheologic and microrheologic parameters

Abstract Whole blood filtration, mean erythrocyte aggregation, and erythrocyte membrane fluidity were examined in 10 patients with vascular atherosclerotic disease (VAD) and in 15 VAD patients with noninsulin-dependent diabetes mellitus (NIDDM) treated with mesoglycan (100 mg orally twice a day) for 30 days. The red cell membrane transverse fluidity gradient was evaluated in the VAD subjects, and the red cell membrane protein lateral mobility was measured in the VAD subjects with NIDDM. In both groups, mesoglycan treatment was responsible for an increase in erythrocyte membrane fluidity. This finding, which reflects an improvement in the dynamic properties of red cell membrane, assumes part…

Erythrocyte Ca2+ content and red cell membrane transverse fluidity gradient in a group of subjects with chronic renal failure (CRF)

Erythrocyte membrane fluidity during mesoglycan treatment

Vascular atherosclerotic disease: Behaviour of the red cell phospholipids and their relationships with the erythrocyte membrane fluidity. Preliminary report

Red cell phospholipids and membrane microviscosity in diabetics

Red cell metabolic parameters and rheological determinants in essential hypertension

Red cell Ca2+ content (cytosolic and total) and erythrocyte membrane fluidity in vascular atherosclerotic disease

Red cell membrane fluidity and red cell aggregation in vascular atherosclerotic disease

Survey of neonatal respiratory care and surfactant administration in very preterm infants in the Italian neonatal network

INTRODUCTION: Variation of respiratory care is described between centers around the world. The Italian Neonatal Network (INN), as a national group of the Vermont-Oxford Network (VON) allows to perform a wide analysis of respiratory care in very low birth weight infants. METHODS: We analyzed the dataset of infants enrolled in the INN in 2009 and 2010 and, for surfactant administration only, from 2006 to 2010 from 83 participating centers. All definitions are those of the (VON). A questionnaire analysis was also performed with a questionnaire on centers practices. RESULTS: We report data for 8297 infants. Data on ventilator practices and outcomes are outlined. Variation for both practices and…

Microrheological determinants and erythrocyte membrane phospholipids in vascular atherosclerotic disease (VAD)