0000000000280477
AUTHOR
R. Brehm
Muscarinic Receptor Activation Increases Efflux of Choline from Isolated Heart and Rat Cortex in Vivo. Interactions with Forskolin and IBMX
Muscarinic receptor activation modulates functions of the heart and neurotransmission in the peripheral and central nervous system. Moreover, muscarinic agonists produce changes in the metabolism of, for example, heart tissue, such as inhibition of beta-adrenoceptor-mediated cAMP accumulation, glycogenolysis and lipase activation.
Choline Fluxes to and from the Rat Cerebral Cortex Studied with the “Cup Technique” in Vivo
Since MacIntosh and Oborin (11) and later Mitchell (12) introduced the “cup technique” as a mean to study acetylcholine release from the cerebral cortex in vivo, this technique has been widely used for investigating the release of various neurotransmitters in anaesthetized as well as unanaesthetized mammals (2, 13, 14). Recently we proposed the “cup technique” as a way for studying the efflux of endogenous choline (Ch) from the rat cerebral cortex (4, 5) and to estimate changes in the extracellular concentration of Ch, if we consider the fluid filling the cup as an extension of the extracellular space.