Molecular Phylogeny of Tintinnid Ciliates (Tintinnida, Ciliophora)
We investigated the phylogeny of tintinnids (Ciliophora, Tintinnida) with 62 new SSU-rDNA sequences from single cells of 32 marine and freshwater species in 20 genera, including the first SSU-rDNA sequences for Amphorides, Climacocylis, Codonaria, Cyttarocylis, Parundella, Petalotricha, Undella and Xystonella, and 23 ITS sequences of 17 species in 15 genera. SSU-rDNA phylogenies suggested a basal position for Eutintinnus, distant to other Tintinnidae. We propose Eutintinnidae fam. nov. for this divergent genus, keeping the family Tintinnidae for Amphorellopsis, Amphorides and Steenstrupiella. Tintinnopsis species branched in at least two separate groups and, unexpectedly, Climacocylis branc…
Sinophysis and Pseudophalacroma are Distantly Related to Typical Dinophysoid Dinoflagellates (Dinophysales, Dinophyceae)
Dinophysoid dinoflagellates are usually considered a large monophyletic group. Large subunit and small subunit (SSU) rDNA phylogenies suggest a basal position for Amphisoleniaceae (Amphisolenia,Triposolenia) with respect to two sister groups, one containing most Phalacroma species plus Oxyphysis and the other Dinophysis,Ornithocercus, Dinophysoid dinoflagellates are usually considered a large monophyletic group. Large subunit and small subunit (SSU) rDNA phylogenies suggest a basal position for Amphisoleniaceae (Amphisolenia,Triposolenia) with respect to two sister groups, one containing most Phalacroma species plus Oxyphysis and the other Dinophysis,Ornithocercus, Histioneis,Citharistes an…
Prvi nalazi dinoflagelata roda Dicroerisma (Actiniscales, Dinophyceae) na Sredozemnom moru
This study is the first to describe and illustrate the dinoflagellate genus Dicroerisma in the Mediterranean Sea. Twenty individuals ascribed to Dicroerisma psilonereiella were encountered. The highest abundance (12 cells L-1) was recorded in the Balearic Sea at 50 m depth. Other specimens were found in subsurface waters (>75 m depth, with some records at 250 m depth) in the Ionian and Levantine Basin. These findings suggest that Dicroerisma is a widespread genus and its presence has gone unnoticed due to its small size, deep water distribution and misidentification with other gymnodinioid cells.
REINSTATEMENT OF THE DINOFLAGELLATE GENUS Tripos TO REPLACE Neoceratium, MARINE SPECIES OF Ceratium (DINOPHYCEAE, ALVEOLATA)
Morphological and molecular data support the split at the genus level of the marine and freshwater species of the dinoflagellate Ceratium (Gonyaulacales, Dinophyceae). A new genus name Neoceratium F. Gomez, D. Moreira & P. Lopez-Garcia was proposed for the marine species. However, it is considered illegitimate (type species is type of Biceratium Vanhoffen), and the nomenclatural priority is here given for the genus Tripos Bory (type species Tripos muelleri ) which replaces Neoceratium . New combinations are proposed for the existing names of species and infraspecific taxa. Reinstalacion del genero de dinoflagelados Tripos para reemplazar a Neoceratium , especies marinas de Ceratium (Dinophy…
Molecular phylogeny of the marine dinoflagellate genus Heterodinium (Dinophyceae)
The dinoflagellate genus Heterodinium has unusual morphological characters such as a mid-ventral intercalary plate with a pore, a small plate in the left side of the dorsal epitheca, three antapical plates, and a well-developed anterior cingular list. We obtained the first SSU rDNA sequences from single cells of six species of Heterodinium from Mediterranean coastal and open waters. They included the type species H. scrippsii and H. rigdeniae and representatives of the other subgenera, Sphaerodinium (H. doma, H. milneri, H. globosum) and Platydinium (H. pavillardii). SSU rDNA phylogeny showed that Heterodinium spp. formed a well-supported monophyletic group (100% bootstrap support) composed…
Diversity and Distribution of the Dinoflagellates Brachidinium, Asterodinium and Microceratium (Brachidiniales, Dinophyceae) in the open Mediterranean Sea
Diversity and Distribution of the DinoflagellatesBrachidinium, AsterodiniumandMicroceratium(Brachidiniales, Dinophyceae) in the open Mediterranean SeaBrachidiniacean dinoflagellates have been investigated in the open waters of the Mediterranean Sea, along a transect from the south of France to the south of Cyprus (20 June-18 July 2008).BrachidiniumandKarenia papilionaceaoften co-occurred,B. capitatumpredominating in the surface waters. The highest abundance ofBrachidiniumwere found in the upper 25min the western Mediterranean with amaximum (24 cells L-1) at a depth of 5 m in the Balearic Sea.Asterodinium(up to 4 cells L-1) was recorded below of deep chlorophyll maxima. The genusMicroceratiu…
A checklist and classification of living dinoflagellates (Dinoflagellata, Alveolata)
A checklist and classification of the extant dinoflagellates are given. Dinokaryotic dinoflagellates (including Noctilucales) comprised 2,294 species belonging to 238 genera. Dinoflagellates sensu lato (Ellobiopsea, Oxyrrhea, Syndinea and Dinokaryota) comprised 2,377 species belonging to 259 genera. The nomenclature of several taxa has been corrected according to the International Code of Botanical Nomenclature. When gene sequences were available, the species were classified following the Small and Large SubUnit rDNA (SSU and LSU rDNA) phylogenies. No taxonomical innovations are proposed herein. However, the checklist revealed that taxa distantly related to the type species of their genera …
Valor de la proteína C reactiva en la detección de la necrosis en la pancreatitis aguda
Objetivos Valorar la utilidad de la proteina C reactiva (PCR) en la deteccion de la necrosis en la pancreatitis aguda y precisar el mejor nivel de corte de la PCR para esta finalidad. Material y metodos Se incluyeron, de forma retrospectiva, 157 pacientes con pancreatitis aguda a los que se habia efectuado una tomografia computarizada (TC) con contraste intravenoso, entre las 72 h y el octavo dia del inicio de la sintomatologia, y determinado el valor de la PCR en el suero por nefelometria a partir de las 24 h del comienzo del dolor. Resultados En total, fueron incluidos 94 varones y 63 mujeres, con una media de edad de 61 anos (limites, 15-96 anos). La etiologia de la pancreatitis fue: lit…
A quantitative review of the lifestyle, habitat and trophic diversity of dinoflagellates (Dinoflagellata, Alveolata)
This study reviews the trends in the lifestyle, habitat distribution and trophic diversity of the 2377 described species of dinoflagellates (Dinophyceae). Most of the dinoflagellates inhabit marine waters, whereas 17% of the total species have colonized continental waters. Dinoflagellates are dominated by planktonic species, while benthic forms represented 8% of the species. From the total number of species, 49% are heterotrophic (devoid of plastids), while 51% of the species have been reported with plastids (that does not strictly imply autotrophy). All the basal dinoflagellates (ellobiopsids, Duboscquodinida, Syndiniales) are heterotrophic, with the exception of a few Noctilucales (Spatul…
Database of diazotrophs in global ocean: abundance, biomass and nitrogen fixation rates
Marine N<sub>2</sub> fixing microorganisms, termed diazotrophs, are a key functional group in marine pelagic ecosystems. The biological fixation of dinitrogen (N<sub>2</sub>) to bioavailable nitrogen provides an important new source of nitrogen for pelagic marine ecosystems and influences primary productivity and organic matter export to the deep ocean. As one of a series of efforts to collect biomass and rates specific to different phytoplankton functional groups, we have constructed a database on diazotrophic organisms in the global pelagic upper ocean by compiling about 12 000 direct field measurements of cyanobacterial diazotroph abundances (based on microscopic …
Esteatosis hepática en la hepatitis crónica por virus C: estudio de los factores de riesgo y relación con el estadio de fibrosis
Fundamento y objetivo La esteatosis hepatica (EH) es un hallazgo histologico frecuente en la infeccion cronica por el virus de la hepatitis C (VHC) que puede influir en el pronostico de la enfermedad. El objetivo del presente estudio prospectivo es valorar los factores asociados a su presencia y su relacion con el estadio de fibrosis en pacientes con infeccion cronica por VHC. Material y metodo Se incluyo a 84 pacientes consecutivos con ARN-VHC positivo, no tratados previamente y a los que se practico una biopsia hepatica. En todos los pacientes se recogieron en el momento de la biopsia variables demograficas, clinicas, analiticas, virales e histologicas. Se practicaron analisis bivariante …
Pancreas and Cystic Fibrosis: The Implications of Increased Survival in Cystic Fibrosis
Background: In acute pancreatitis (AP), pancreatic necrosis (PN) is an important local complication that can be identified by means of contrast-enhanced computed tomography (CECT).
99mTc-Hexamethylpropylene Amineoxime Leukocyte Scintigraphy in Acute Pancreatitis: An Alternative to Contrast-Enhanced Computed Tomography?
Contrast-enhanced computed tomography (CECT) is the most efficient imaging technique for the diagnosis and staging of acute pancreatitis (AP); its use, however, may be unfeasible in some patients as a consequence of the drawbacks of intravenous (IV) contrast material. The aim of this study was to test the utility of labeled leukocyte scintigraphy (LLS) as an alternative imaging technique to CECT for the staging of AP.Sixty-six patients with AP were prospectively studied. All patients underwent CECT and pancreatic LLS using (99m)Tc-hexamethylpropylene amineoxime as leukocyte label within a time interval of 2 days, in the early phase of AP. In addition, all patients had their serum C-reactive…
M.U investigación en CC de la actividad física y el deporte 2012. (Mesa proyectos Estic)
Taula rodona de projectes Estic.''M.U investigación en CC de la actividad física y el deporte 2012'', presentat per Fernando Gómez Gonzalvo en el marc de les III Trobades d'Innovació Educativa de la Universitat de València. Producción:CREAM (http://cream.uv.es)-SFPIE(www.uv.es/sfpie)-VLC Campus