0000000000372699
AUTHOR
Carlos Simon
Additional file 7 of Endometrial microbiota composition is associated with reproductive outcome in infertile patients
Additional file 6 : Supplementary Table 3. Sequencing reads obtained from endometrial fluid and endometrial biopsy samples.
Additional file 8 of Endometrial microbiota composition is associated with reproductive outcome in infertile patients
Additional file 7 : Supplementary Table 4. Bacteria detected in endometrial fluid and endometrial biopsy samples after filtering for low abundance taxa and potential contaminants.
Single cell RNAseq provides a molecular and cellular cartography of changes to the human endometrium through the menstrual cycle
SummaryIn a human menstrual cycle, the endometrium undergoes remodeling, shedding, and regeneration, all of which are driven by substantial gene expression changes in the underlying cellular hierarchy. Despite its importance in human fertility and regenerative biology, mechanistic understanding of this unique type of tissue homeostasis remains rudimentary. We characterized the transcriptomic transformation of human endometrium at single cell resolution, dissecting the multidimensional cellular heterogeneity of this tissue across the entire natural menstrual cycle. We profiled the behavior of 6 endometrial cell types, including a previously uncharacterized ciliated epithelial cell type, duri…
Additional file 5 of Endometrial microbiota composition is associated with reproductive outcome in infertile patients
Additional file 4 : Supplementary Table 1. Sociodemographic and clinical characteristics of study participants.
Single-cell RNA Expression of SARS-CoV-2 Cell Entry Factors in Human Endometrium during Preconception
AbstractWe investigated potential SARS-CoV-2 tropism in human endometrium by single-cell RNA-sequencing of viral entry-associated genes in healthy women. Percentages of endometrial cells expressing ACE2, TMPRSS2, CTSB, or CTSL were <2%, 12%, 80%, and 80%, respectively, with 0.7% of cells expressing all four genes. Our findings imply low efficiency of SARS-CoV-2 infection in the endometrium before embryo implantation, providing information to assess preconception risk in asymptomatic carriers.
Monitoring Stemness in Long-Term hESC Cultures by Real-Time PCR
Human embryonic stem cells (hESC) involve long-term cultures that must remain undifferentiated. The real-time PCR (RT-PCR) technique allows the relative quantification of target genes, including undifferentiation and differentiation markers when referred to a housekeeping control with the addition of a calibrator that serves as an internal control to compare different lots of reactions during the time. The main aspects will include a minimal number of cells to be analyzed, genes to be tested, and how to choose the appropriate calibrator sample and the reference gene. In this chapter, we present how to apply the RT-PCR technique, protocols for its performance, experimental set-up and softwar…
Additional file 6 of Endometrial microbiota composition is associated with reproductive outcome in infertile patients
Additional file 5 : Supplementary Table 2. Clinical variables in patients with different reproductive outcomes.
The Endometrial Microbiome and Its Impact on Human Conception
Changes in the female genital tract microbiome are consistently correlated to gynecological and obstetrical pathologies, and tract dysbiosis can impact reproductive outcomes during fertility treatment. Nonetheless, a consensus regarding the physiological microbiome core inside the uterine cavity has not been reached due to a myriad of study limitations, such as sample size and experimental design variations, and the influence of endometrial bacterial communities on human reproduction remains debated. Understanding the healthy endometrial microbiota and how changes in its composition affect fertility would potentially allow personalized treatment through microbiome management during assisted…
Additional file 4 of Endometrial microbiota composition is associated with reproductive outcome in infertile patients
Additional file 3 : Supplementary Figure 3. Confidence intervals and reference ranges of bacterial taxa detected in patients with live birth. The endometrial microbiome from patients with a live birth was analysed in (A) endometrial fluid and (B) endometrial biopsy samples to determine the reference ranges for each evaluated taxon. The confidence interval displays the relative abundance for all assessed patients, revealing the healthy ranges of abundance for the taxa in the tested panel. The healthy distribution was used to define the 95% confidence interval (red line) and taxa abundance in patients with poor reproductive outcomes���no pregnancy, biochemical pregnancy, and clinical miscarri…
Additional file 3 of Endometrial microbiota composition is associated with reproductive outcome in infertile patients
Additional file 2 : Supplementary Figure 2. Taxa enriched in patients with different reproductive outcomes.
Additional file 2 of Endometrial microbiota composition is associated with reproductive outcome in infertile patients
Additional file 1 : Supplementary Figure 1. Taxa found in endometrial fluid (A) and endometrial biopsy (B) samples.