0000000000513970
AUTHOR
Marilisa Barranca
showing 28 related works from this author
Production of an egg yolk antibody against Parietaria judaica 2 allergen
2009
Specific antibodies are essential tools for studying proteins as well as for diagnostic research in biomedicine. The egg yolk of immunized chicken is an inexpensive source of high-quality polyclonal antibodies. The 12-kDa Parietaria judaica 2 allergen was expressed as a fusion protein and was used to immunize Leghorn chickens. In this paper, we show, using 2-dimensional gel electrophoresis and immunoblotting, that chicken antibodies raised against a recombinant allergen can be used to recognize similar proteins from a pollen raw extract. Allergen identity was confirmed by nanoLC-nanospray-tandem mass spectrometry analysis. Our data demonstrate for the first time that a synergistic combinati…
Proteomic analysis of Parietaria judaica pollen and allergen profiling by an immunoproteomic approach
2010
Parietaria judaica pollen is a common cause of airway allergic disease in the Mediterranean area. Proteome analysis of mature Parietaria judaica pollen by two-dimensional gel electrophoresis (2-DE) and mass spectrometry has established the first reference proteome map of this weed. Proteins involved in a variety of cellular functions as well as the occurrence of allergens were detected. By using 2-DE and immunoblotting with sera from Parietaria judaica allergic patients we obtained a more detailed characterization of Parietaria judaica allergen profile so to improve our comprehension of the pathogenesis of pollen-induced allergic reaction.
Identification of Biomarkers in Cerebrospinal Fluid and Serum of Multiple Sclerosis Patients by Immunoproteomics Approach
2014
Multiple sclerosis (MS) is an autoimmune inflammatory demyelinating disease of the central nervous system. At present, the molecular mechanisms causing the initiation, development and progression of MS are poorly understood, and no reliable proteinaceous disease markers are available. In this study, we used an immunoproteomics approach to identify autoreactive antibodies in the cerebrospinal fluid of MS patients to use as candidate markers with potential diagnostic value. We identified an autoreactive anti-transferrin antibody that may have a potential link with the development and progression of MS. We found this antibody at high levels also in the serum of MS patients and created an immun…
Zymographic analysis of circulating and tissue forms of colon carcinoma gelatinase A (MMP-2) and B (MMP-9) separated by mono- and two-dimensional ele…
2001
Gelatinase A (MMP-2) and gelatinase B (MMP-9) play a key role in the proteolytic cascade leading to ECM degradation during invasion and metastasis. The enzyme activity is regulated both at the intra- and extra-cellular level. Extracellular regulation is achieved mainly through the balance between proenzyme activation and inhibition, which appears to be altered in cancer patients. One of the mechanisms of MMP inhibition is the binding of the enzymes to appropriate tissue inhibitors (TIMP). In the recent literature, it has been suggested that MMP-2 and/or MMP-9 are indeed over-produced in many carcinomas, while the identity of the various enzymatic forms (latent, activated and enzyme/inhibito…
Comparative Proteome Profiling and Functional Analysis of Chronic Myelogenous Leukemia Cell Lines
2007
The aim of the present study was the molecular profiling of different Ph+ chronic myelogenous leukemia (CML) cell lines (LAMA84, K562, and KCL22) by a proteomic approach. By employing two-dimensional gel electrophoresis combined with mass spectrometry analysis, we have identified 191 protein spots corresponding to 142 different proteins. Among these, 63% were cancer-related proteins and 74% were described for the first time in leukemia cells. Multivariate analysis highlighted significant differences in the global proteomic profile of the three CML cell lines. In particular, the detailed analysis of 35 differentially expressed proteins revealed that LAMA84 cells preferentially expressed prot…
Effects of Parietaria judaica pollen extract on human microvascular endothelial cells
2008
Abstract Pollinosis from Parietaria judaica is one of the main causes of allergy in the Mediterranean area. The present study is designed to assess if P. judaica pollens contain bioactive compounds able to elicit a functional response in endothelial cells. We have demonstrated that addition of pollen extract to human lung microvascular endothelial cells (HMVEC-L) induces a modification of cell morphology, actin cytoskeletal rearrangements and an increase in endothelial cell permeability. We further showed that the treatment of endothelial cells with pollen extract causes an increase of E-selectin and VCAM-1 protein levels as well as an increase of IL-8 production. The stimulation of cell–ce…
Profilo proteomico del polline di Parietaria judaica
2007
Proteome profiling of mature pollen of Parietaria judaica.
2007
Analysis of cellular signalling and proteomic profiling in Imatinib-resistant chronic myeloid leukemia cells following treatment with carboxyamidotri…
2006
Effects of Parietaria judaica on human lung microvascular endothelial cells
2008
Analisi proteomica del polline di Parietaria judaica
2006
Molecular characterization of imatinib-resistant CML cells lines by proteomic profiling
2006
Cross-talk between fibroblast and breast cancer cells: a proteomic study
2003
Molecular profiling of imatinib resistant chronic myelogenous leukemia cells
2010
Zymographic detection of matrix proteases in breast cancer biopsies by mono- and two-dimensional electrophoresis
2004
Molecular characterization of imatinib-resistant CML cell lines by proteomic profiling
2006
Analisi comparativa del profilo proteomico di cellule di leucemia mieloide cronica resistenti all’imatinib dopo trattamento con carbossiamidotriazolo…
2006
Application of an immunoproteomic approach to detect anti-profilin antibodies in sera of paritaria judaica allergic patients
2011
Pollen from grasses, weeds, and trees constitutes one of the main sources of inhalant allergens frequently associated with seasonal patterns of allergic diseases. Pollen allergens show some analogies in the amino acids sequence which determine immunological similarity and cross reactivity. Parietaria judaica (P.j) pollen represents one of the main sources of allergens in the Mediterranean area and its major allergens have already been identified (Par j 1 and Par j 2). Recently, has been also described a minor allergen, profilin (Par j 3), an allergen present in pollen of trees, grasses and weeds. Allergenic plant profilins constitute a highly conserved family with sequence identities of 70%…
Caratterizzazione del profilo proteomico di linee cellulari di leucemia mieloide cronica resistenti all'imatinib
2006
Proteomics and proteomic modulations of breast cancer cells
2004
Effect of Parietaria judaica pollen on human lung microvascular endothelial cells.
2008
Profilo proteomico ed analisi funzionale di linee cellulari di leucemia mieloide cronica
2008
Involvement of gelatinases in breast cancer: correlation with clinical parameters and 2D-IPG applications.
2004
Metodo e kit per la sclerosi multipla
2011
E’ descritta la scoperta di anticorpi anti-transferrina come biomarcatori per la sclerosi multipla da cui è stato messo a punto un metodo di diagnosi, prognosi, monitoraggio della terapia e relativo kit.