Experimental Assessment of the Backoff Behavior of Commercial IEEE 802.11b Network Cards

It has been observed that different IEEE 802.11 commercial cards produced by different vendors experience different performance, either when accessing alone the channel, as well as when competing against each other. These differences persist also when thorough measurement methodologies (such as RF shielding, laptop rotation, etc) are applied, and alignment of the environmental factors (same laptop models, traffic generators, etc) is carried out. This paper provides an extensive experimental characterization of the backoff operation of six commercial NIC cards. It suggests a relevant methodological approach, namely a repeatable, well defined, set of experiments, for such a characterization. …

FPGA-based concurrent watchdog for real-time control systems

A straightforward and efficient implementation of a custom concurrent watchdog processor for real-time control systems is presented. Emphasis is given to the techniques used for on-line checking the main processor activity without adding overhead, and to the advantages of a field programmable gate array implementation.

The Monte San Nicola section (Sicily) revisited: A potential unit-stratotype of the Gelasian Stage

The Monte San Nicola area (Southern Sicily) offers a spectacular exposure of open-marine sediments that were employed in 1998 for defining the Global Stratotype Section and Point (GSSP) of the Gelasian Stage (Upper Pliocene). After the lowering of the Pliocene/Pleistocene boundary to ca. 2.6 Ma in 2010, the Gelasian GSSP has been redefined as the base of both the Pleistocene Series and the Quaternary Period, which increased its importance and visibility within the scientific community. However, documentation on the Monte San Nicola reference section is still sparse. In the light of its renewed status, we decided to undertake a complete revision of the Gelasian Stage in its type area, in ord…

Contract for the support of household appliances control and monitoring application interworking specification

A two-end traveling wave fault location system for MV cables based on LoRa technology

In this paper a new low cost device is proposed for fault location in power distribution system. The device is part of a system that should include several units installed in couples at the two ends of MV cable lines. Their low cost justifies a widespread installation of these devices, moreover other functionalities could be considered so as to enrich the potential of the system for MV cables monitoring and asset management. The system exploits a two-end traveling wave technique, low complexity and low cost solutions both for the analog front end as well as for the communication infrastructure. A LoRa network is indeed considered as wireless modulation technique perfectly suited when commun…

Novel in vitro and in vivo data on the cellular localization of Hsp10 in smokers affected by COPD and in lung-derived cell lines exposed to cigarette smoke extract as stressor.

Cigarette smoke is a potent stressor for the respiratory system, contributing to pathogenesis, for instance in chronic obstructive pulmonary disease (COPD), but its effects on the expression, function, and cellular localization of mitochondrial chaperonins are still largely unknown. We studied in vivo (airways biopsies) the localization of Hsp10 and Hsp60 in patients (smokers and non-smokers) affected by mild-moderate COPD, and characterized the effects of non-lethal doses of cigarette smoke extract (CSE) on the expression of these molecules in two human cell lines: lung fibroblasts (HFL-1) and bronchial epithelial (16HBE). We applied various in vitro methods: immunohistochemistry (IHC), su…

Chaperone patterns in vernal keratoconjunctivitis are distinctive of cell and Hsp type and are modified by inflammatory stimuli

Background Vernal keratoconjunctivitis (VKC) is a severe ocular allergy with pathogenic mechanism poorly understood and no efficacious treatment. The aims of the study were to determine quantities and distribution of Hsp chaperones in the conjunctiva of VKC patients and assess their levels in conjunctival epithelial and fibroblast cultures exposed to inflammatory stimuli. Methods Hsp10, Hsp27, Hsp40, Hsp60, Hsp70, Hsp90, Hsp105, and Hsp110 were determined in conjunctiva biopsies from nine patients and nine healthy age-matched normal subjects, using immunomorphology and qPCR. Conjunctival epithelial cells and fibroblasts were cultured and stimulated with IL-1β, histamine, IL-4, TNF-α, or UV-…

Liquid Biopsy in Colorectal Cancer

Colorectal cancer (CRC) is the third most common cancer worldwide. Oncological CRC treatment is changing gradually replacing ancient drugs with the newest targeted agents (target therapy) and immunotherapy. The correct use of these molecular agents is based on a series of genetic evaluations able to predict treatment response. The tissue evaluation of these predictors is generally fully of serious limitations among which the invasiveness of the tissue biopsy procedure and its non-informative quality compared to the entire neoplastic mass. For these reasons, liquid biopsy could represent an ideal tool to obtain easily and quickly the necessary information to decide the best therapeutic strat…

Association of increased CCL5 and CXCL7 chemokine expression with neutrophil activation in severe stable COPD

BACKGROUND: Increased numbers of activated neutrophils have been reported in the bronchial mucosa of patients with stable chronic obstructive pulmonary disease (COPD), particularly in severe disease. OBJECTIVES: To investigate the expression of neutrophilic chemokines and adhesion molecules in bronchial biopsies from patients with stable COPD of different severity (GOLD stages I-IV) compared with age-matched control subjects, smokers with normal lung function and never smokers. METHODS: The expression of CCL5, CXCL1, 5, 6, 7 and 8, CXCR1, CXCR2, CD11b and CD44 was measured in the bronchial mucosa using immunohistochemistry, confocal immunofluorescence, real-time quantitative polymerase chai…

Human Wharton's jelly-derived mesenchymal stem cells express several immunomodulatory molecules both in their naïve state and hepatocyte-like differentiated progeny: prospects for their use in liver diseases

Wharton’s jelly (WJ), the main constituent of umbilical cord, is a reliable source of mesenchymal stem cells (MSC). WJ-MSC show unique ability in crossing lineage borders. As other extraembryonic mesenchymal populations (placenta and amnionderived cells), WJ-MSC express several immunomodulatory molecules, essential during the initial phases of human development. Indeed, our recent work pointed out the expression of non-classical HLA molecules as HLA-G in such cells, together with a favorable combination of B7 costimulators. Very few data in literature suggest that some of the immune features of the naïve cells are maintained after performing differentiation. The aim of this work was extendi…

Wide bandwidth impedance meter using low rate random sampling

A novel impedance measurement method based on random sampling of voltage and current signals is proposed. This technique dramatically reduces the sampling frequency requirements, thus circumventing the limitations imposed by maximum speed of the analog to digital converter and the signal processing unit. The lowering of the sampling frequencies allows the design and the implementation of an almost all digital architecture by using a simple microprocessor based embedded system and a digital frequency synthesizer. The basic principles are presented, and the implemented algorithms are described. Experimental results show the instrument performances compared to others commercial alternatives.

The pathogenetic role of the chaperonin Hsp60 in chronic obstructive pulmonary disease: new data and perspectives

Contribution of bronchial biopsies in the evaluation of pathogenesis and progression of COPD.

This review summarizes and discusses the lung pathology of COPD patients emphasising on inflammatory cell phenotypes and mechanisms which prevail in different clinical conditions. In bronchial biopsies a series of events takes place during the progression of the disease from mild to severe. T-lymphocytes, particularly CD8+ cells and macrophages are the prevalent inflammatory cells in the lungs of healthy smokers and patients with mild/moderate COPD. This T-cell activation seems to be sustained by CD4+, CD8+ cells and macrophages expressing transcription factors and Tc1 cytokines such as NF-kB, STAT4 and IFNγ. In contrast, severe disease is characterized by lymphocytes producing greater amo…

Energy Management via Connected Household Appliances

The study reported hereafter provides results based on a campaign of simulations conducted by University of Palermo on the basis of its knowledge in the field Energy Value Chain and in the field of Energy Management (EM) for household appliances. Inputs from CECED Convergence WG has been fundamental to base that study on realistic data and cases. Moreover, the so called EM study, offers also a clear picture about the opportunity to have energy management applications and services in house, being those opportunities strictly related to the cost of in- frastructure needed and to the benefits belonging to different actors in the energy value chain. Energy savings have been considered both in t…

Survivin is regulated by interleukin-4 in colon cancer stem cells

Colorectal cancer has provided an important model to test the stem cell hypothesis of cancer origin, which implies that cancer arises as a result of genetic aberrations in stem cells leading to deregulation of the proliferation/differentiation balance. We and others have demonstrated that, similarly to other solid tumors, colon carcinogenesis and progression are dictated by highly apoptosis-resistant stem-like cells. Our data have suggested that protection from apoptosis is achieved by autocrine production of interleukin-4 (IL-4) through up-regulation of anti-apoptotic mediators. In this study, we extend our analysis to another apoptosis inhibitor widely expressed in tumors, namely survivin…

An FPGA-Based Software Defined Radio Platform for the 2.4GHz ISM Band

A prototype of a Software Defined Radio (SDR) platform has been successfully designed and tested implementing a reconfigurable IEEE 802.11 and ZigBee receiver. The system exploits the reconfiguration capability of an FPGA for implementing a number of receiver configurations that share the same RF front-end. Configurations can be switched at run time, or can share the available logic and radio resource.

An Experimental Testbed and Methodology for Characterizing IEEE 802.11 Network Cards

It has been observed that IEEE 802.11 commercial cards produced by different vendors show a different behavior in terms of perceived throughput or access delay. Performance differences are evident both when the cards contend alone to the channel, and when heterogeneous cards contend together. Since the performance misalignment does not disappear by averaging the environmental factors (such as propagation conditions, laptop models, traffic generators, etc), it is evident that the well known throughput-fairness property of the DCF protocol is not guaranteed in actual networks. In this paper we propose a methodological approach devised to experimentally characterize the IEEE 802.11 commercial …

Misfire Detection System based on the Measure of Crankshaft Angular Velocity

Misfire detection systems are becoming increasingly important in automotive market due to recent environmental issues (Euro rules). An early misfire diagnosis also allows to prevent damages to the exhaust emission system and consequent costs for the user. Today few low cost methods exists in order to precisely detect single misfires in real time, the majority in fact require the use of expensive sensors (e.g. pressure sensors) or dedicated circuits (e.g. ionization current sensing). This work describes a method and electronic system capable of detecting misfires with good accuracy, using parameters such as the speed sensor signal, already available in commercial engines. The proposed method…

Airway inflammation in healthy smokers

Cigarette smoking is a risk factor associated with lung cancer and many other neoplasms of various organs, coronary artery disease and numerous vascular disorders, Chronic Obstructive Pulmonary Disease (COPD) and other types of lung diseases [13]. Much work has been done on the in vitro and ex vivo carcinogenetic effects of tobacco smoking while the effects of smoking on innate and adaptive immune function have been studied to a lesser degree. Recent data suggests that cigarette smoke alters the functions of the immune system and increases susceptibility to viral and bacterial infections [4-6]. In the respiratory system along the last years many studies have depicted the changes induced by …

Reconfigurable digital instrumentation based on FPGA

A novel application of FPGA to realize digital test equipment is proposed. It takes advantage of the dynamic reconfigurability of FPGAs so easily tailoring custom test functions in the same instrument. This results in high effective, compact and low cost instruments.

The Burdigalian GSSP: the missing tile to complete the Neogene interval of the Geological Time Scale

The effects of LIPUS on ctDNA release in the medium of NSCLC cell lines

On the Fidelity of IEEE 802.11 commercial cards

The IEEE 802.11 D CF protocol is known to be fair in terms of long-term resource repartition among the contending stations. However, when considering real scenarios, where commercial 802.11 cards interact, very unpredictable as well as sometimes surprising behaviors emerge. Motivation of this paper is to investigate the reasons of the very evident disagreement between the theoretical IEEE 802.11 DCF protocol models and its practical implementations. Inparticular, we try to characterize the card behavior not only in terms of perceived throughput, but also in terms of low-level channel access operations. In fact, the simple throughput analysis does not allow to identify what affecting paramet…

Integration of distributed on site control actions via combined photovoltaic and solar panels system

A systematic evaluation of the potential energy saving and CO2 emissions reduction on buildings was carried out, when a renewable energy system is locally installed. The exploitation of a combined solar and photovoltaic system and the integration of suitable energy management actions were deeply investigated. A model of these solar and photovoltaic components has been developed and included within a complete house simulator, capable to precisely figure out the daily electric energy consumptions of typical apartments and to measure the positive actions induced by the added renewable energy. Saving up to 40% on the energy consumptions and even more savings on wiring losses seems to be easily …

In-situ gelling xyloglucan formulations as 3D artificial niche for adipose stem cell spheroids.

Abstract Three-dimensional spheroidal cell aggregates of adipose stem cells (SASCs) are a distinct upstream population of stem cells present in adipose tissue, with enhanced regeneration properties in vivo. The preservation of the 3D structure of the cells, from extraction to administration, can be a promising strategy to ensure optimal conditions for cell viability and maintenance of stemness potential. With this aim, an artificial niche was created by incorporating the spheroids into an injectable, in-situ gelling solution of partially degalactosylated xyloglucan (dXG) and an ad hoc formulated culture medium for the preservation of stem cell spheroid features. The evolution of the mechani…

Chaperones in disease: quantitative changes in chronic obstructive pulmonary disease (COPD)

Nerve ablation after bronchial thermoplasty and sustained improvement in severe asthma

Abstract Background Bronchial thermoplasty (BT) is a non-pharmacological intervention for severe asthma whose mechanism of action is not completely explained by a reduction of airway smooth muscle (ASM). In this study we analyzed the effect of BT on nerve fibers and inflammatory components in the bronchial mucosa at 1 year. Methods Endobronchial biopsies were obtained from 12 subjects (mean age 47 ± 11.3 years, 50% male) with severe asthma. Biopsies were performed at baseline (T0) and after 1 (T1), 2 (T2) and 12 (T12) months post-BT, and studied with immunocytochemistry and microscopy methods. Clinical data including Asthma Quality of Life Questionnaire (AQLQ) and Asthma Control Questionnai…

Isolation and characterization of pluripotent cells from the subendocardial layer of human hearts from chronic heart failure patients

Technical Aspects for the Evaluation of Circulating Tumor Cells (CTCs)

Liquid biopsy is considered a valid strategy to improve patients’ selection and treatment monitoring in clinical practice. Indeed Circulating Tumor Cells (CTCs) represent an important component of liquid biopsy and their clinical evaluation is becoming fundamental for a better patients’ stratification. Several techniques have been developed for CTCs isolation and enrichment and in this chapter we provide an updated overview of the main strategies that can be used.

T helper type 17-related cytokine expression is increased in the bronchial mucosa of stable chronic obstructive pulmonary disease patients.

Summary There are increased numbers of activated T lymphocytes in the bronchial mucosa of stable chronic obstructive pulmonary disease (COPD) patients. T helper type 17 (Th17) cells release interleukin (IL)-17 as their effector cytokine under the control of IL-22 and IL-23. Furthermore, Th17 numbers are increased in some chronic inflammatory conditions. To investigate the expression of interleukin (IL)-17A, IL-17F, IL-21, IL-22 and IL-23 and of retinoic orphan receptor RORC2, a marker of Th17 cells, in bronchial biopsies from patients with stable COPD of different severity compared with age-matched control subjects. The expression of IL-17A, IL-17F, IL-21, IL-22, IL-23 and RORC2 was measure…

Efficient FPGA Implementation of an Adaptive Noise Canceller

A hardware implementation of an adaptive noise canceller (ANC) is presented. It has been synthesized within an FPGA, using a modified version of the least mean square (LMS) error algorithm. The results obtained so far show a significant decrease of the required gate count when compared with a standard LMS implementation, while increasing the ANC bandwidth and signal to noise (S/N) ratio. This novel adaptive noise canceller is then useful for enhancing the S/N ratio of data collected from sensors (or sensor arrays) working in noisy environment, or dealing with potentially weak signals.

High resolution computed tomography quantitation of emphysema is correlated with selected lung function values in stable COPD.

BACKGROUND: The literature shows conflicting results when high-resolution computed tomography (HRCT) scores of emphysema were correlated with different indices of airflow obstruction. OBJECTIVES: We correlated HRCT scores of emphysema with different indices of airflow obstruction. METHODS: We performed HRCT of the chest in 59 patients, all smokers or ex-smokers, with stable chronic obstructive pulmonary disease of different severity [GOLD stages I-IV; mean age ± SD 67.8 ± 7.3 years; pack/years 51.0 ± 34.6; percent predicted forced expiratory volume in 1 s (FEV(1)% predicted) 52.3 ± 17.6; post-bronchodilator FEV(1)% predicted 56.5 ± 19.1; FEV(1)/forced vital capacity (FVC) ratio 50.8 ± 10.2;…

Apoptosis resistance in epithelial tumors is mediated by tumor-cell-derived interleukin-4

We investigated the mechanisms involved in the resistance to cell death observed in epithelial cancers. Here, we identify that primary epithelial cancer cells from colon, breast and lung carcinomas express high levels of the antiapoptotic proteins PED, cFLIP, Bcl-xL and Bcl-2. These cancer cells produced interleukin-4 (IL-4), which amplified the expression levels of these antiapoptotic proteins and prevented cell death induced upon exposure to TRAIL or other drug agents. IL-4 blockade resulted in a significant decrease in the growth rate of epithelial cancer cells and sensitized them, both in vitro and in vivo, to apoptosis induction by TRAIL and chemotherapy via downregulation of the antia…