P129 L’obésité interfère avec la détection oro-sensorielle des acides gras à longue chaîne chez l’homme

2013

The oral lipid sensor GPR120 is not indispensable for the orosensory detectionof dietary lipids in the mouse

2014

International audience; Implication of the long-chain fatty acid (LCFA) receptor GPR120, also termed free fatty acid receptor 4 (FFAR4), in the taste-guided preference for lipids is a matter of debate. To further unravel the role of GPR120 in the "taste of fat", the present study was conducted on GPR120-null mice and their wild-type littermates. Using a combination of morphological (i.e. immunohistochemical staining of circumvallate papillae - CVP), behavioral (i.e. two-bottle preference tests, licking tests and conditioned taste aversion) and functional studies (i.e. calcium imaging in freshly isolated taste bud cells - TBC), we show that absence of GPR120 in oral cavity was not associated…

Is the taste of fat regulated?

2013

Over the last decade, converging data have been accumulated both in rodents and humans, supporting the existence of a sixth taste modality devoted to the perception of dietary lipids. It is well known that the sense of taste is determinant for the food choice and that the overconsumption of highly palatable energy-dense foods contributes to the current obesity epidemic. Thus, an important issue in terms of Public Health is to understand the mechanisms by which the oro-sensory perception of fat is regulated. An overview of our current knowledge in this field of investigations is proposed in this mini-review.

P208: La chute de la détection oro-sensorielle des lipides alimentaires chez la souris obèse a-t-elle une origine inflammatoire ?

2014

Introduction et but de l’etude Nous avons recemment montre qu’une obesite induite chez la souris par un regime riche en acides gras satures perturbe la detection oro-sensorielle des lipides alimentaires. En effet, les souris obeses deviennent incapables de detecter correctement la presence de faibles concentrations de lipides lors de tests de double choix. Une correlation inverse entre masse grasse et seuil de detection orale des lipides a ete trouvee. Ce phenomene, dont l’origine est un dysfonctionnement de la cascade de signalisation CD36 dependante dans la papille gustative caliciforme (CVP), est reversible puisqu’une perte de masse grasse induite par une restriction calorique permet une…

Obesity interferes with the orosensory detection of long-chain fatty acids in humans

2014

Background: The association between the orosensory detection of lipids, preference for fatty foods, and body mass index (BMI; in kg/m 2 ) is controversial in humans. Objective: We explored the oral lipid-sensing system and the orosensory-induced autonomic reflex system in lean and obese subjects. Design: Lean (BMI: 19 to ,25; n = 30) and obese (BMI .30; n = 29) age-matched men were enrolled. Their oral threshold sensitivity to linoleic acid (LA) was determined by using a 3-alternative forced-choice ascending procedure, and their eating habits were established by the analysis of 4 consecutive 24-h food-consumption diaries. The effect of brief oral lipid stimulations on plasma triglyceride [(…

Obesity alters the gustatory perception of lipids in the mouse: plausible involvement of lingual CD36. : Obesity decreases the fat preference

2013

International audience; A relationship between orosensory detection of dietary lipids, regulation of fat intake, and body mass index was recently suggested. However, involved mechanisms are poorly understood. Moreover, whether obesity can directly modulate preference for fatty foods remains unknown. To address this question, exploration of the oral lipid sensing system was undertaken in diet-induced obese (DIO) mice. By using a combination of biochemical, physiological, and behavioral approaches, we found that i) the attraction for lipids is decreased in obese mice, ii) this behavioral change has an orosensory origin, iii) it is reversed in calorie-restricted DIO mice, revealing an inverse …

P073 L’obésité diminue, de manière réversible, la préférence pour les lipides alimentaires chez la souris

2013

International audience; Introduction et but de l’étude. – Au cours des dernières années, il a été suggéré l’existence d’un lien étroit entre la détection oro-sensorielle des lipides alimentaire, la régulation de la prise alimentaire et l’IMC. Toutefois, les mécanismes affectant la sensibilité aux lipides ainsi que l’éventuelle implication directe de l’obésité restent mal connus.

Lipid-mediated release of GLP-1 by mouse taste buds from circumvallate papillae: putative involvement of GPR120 and impact on taste sensitivity

2012

Glucagon-like peptide-1 (GLP-1) signaling modulates sweet-taste sensitivity in the mouse. Because circumvallate papillae (CVPs) express both GLP-1 and its receptor, a local regulation has been suggested. However, whether dietary lipids are involved in this regulation, as shown in the gut, is unknown. By using a combination of biochemical, immunohistochemical, and behavioral approaches, the present data i) confirm the role of GLP-1 signaling in the attraction for sucrose, ii) demonstrate that minute quantities of long-chain FAs (LCFAs) reinforce the attraction for sucrose in a GLP-1 receptor-dependent manner, iii) suggest an involvement of the LCFA receptor GPR120 expressed in taste buds in …

P060 Étude comparative par immunolocalisation des lipido-récepteurs CD36 et GPR120 dans la papille caliciforme de souris

2013

L’obésité interfère avec la détection oro-senorielle des acides gras à longue chaîne chez l’homme

2013

National audience

Obesity interferes with the oro-sensory detection of long-chain fatty acids in Human

2013

International audience