0000000000679065

AUTHOR

Eva Sanchez

showing 3 related works from this author

Defining the genomic signature of totipotency and pluripotency during early human development.

2013

The genetic mechanisms governing human pre-implantation embryo development and the in vitro counterparts, human embryonic stem cells (hESCs), still remain incomplete. Previous global genome studies demonstrated that totipotent blastomeres from day-3 human embryos and pluripotent inner cell masses (ICMs) from blastocysts, display unique and differing transcriptomes. Nevertheless, comparative gene expression analysis has revealed that no significant differences exist between hESCs derived from blastomeres versus those obtained from ICMs, suggesting that pluripotent hESCs involve a new developmental progression. To understand early human stages evolution, we developed an undifferentiation netw…

EmbryologyBlastomeresMicroarraysCellular differentiationGene ExpressionCell Fate DeterminationMolecular Cell BiologyGene Regulatory NetworksInduced pluripotent stem cellreproductive and urinary physiologyGeneticsMultidisciplinarySystems BiologyStem CellsQTotipotentRGenomic signatureCell DifferentiationGenomicsCell biologyFunctional GenomicsBlastocyst Inner Cell MassBlastocyst Inner Cell Massembryonic structuresMedicineResearch ArticlePluripotent Stem CellsSystems biologyCell PotencyScienceEmbryonic DevelopmentBiologyMolecular GeneticsGeneticsHumansGene NetworksBiologyEmbryonic Stem CellsGenome HumanGene Expression ProfilingBio-OntologiesComputational BiologyMolecular Sequence AnnotationComparative GenomicsMolecular DevelopmentEmbryonic stem cellSignalingSignaling NetworksGene expression profilingGenome Expression AnalysisTotipotent Stem CellsDevelopmental BiologyPLoS ONE
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Effect of oocyte morphology on post-warming survival and embryo development in vitrified autologous oocytes.

2019

Abstract Research question Does the presence of dysmorphisms affect post-warming survival and embryo development in vitrified autologous oocytes? Design A retrospective study comparing post-warming survival, fertilization and embryo development between morphologically normal (n = 269) and dysmorphic oocytes (n = 147). Results The survival rate was 81.4% in the morphologically normal oocytes and 87.1% in the dysmorphic oocyte group (OR 1.53; 95% CI 0.86 to 2.72). The fertilization rate was 69.9 versus 66.4% (OR 0.85; 95% CI 0.53 to 1.36), the proportion of good-quality embryos on day 3 was 30.3% versus 32.0% (OR 1.08; 95% CI 0.59 to 1.97) and the blastocyst formation rate was 54.5% versus 60…

0301 basic medicineAdultCell SurvivalEmbryonic DevelopmentBiologyAndrologyEmbryo Culture Techniques03 medical and health sciences0302 clinical medicineHuman fertilizationOvulation InductionPregnancymedicineHumansBlastocystFormation rateSurvival rateRetrospective StudiesCryopreservation030219 obstetrics & reproductive medicineEmbryogenesisObstetrics and GynecologyEmbryoOocyteEmbryo TransferVitrification030104 developmental biologymedicine.anatomical_structureReproductive MedicineOocytesFemaleDevelopmental BiologyReproductive biomedicine online
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Derivation and characterization of three new Spanish human embryonic stem cell lines (VAL −3 −4 −5) on human feeder and in serum-free conditions

2006

A total of 184 human embryos, frozen for >5 years, were donated; informed consent was obtained according to Spanish law 45/2003. Survival rate was 40% and three out of 24 blastocysts (12.5%) developed into putative hESC lines, named VAL-3, VAL-4, and VAL-5. The derivation process was performed on microbiologically tested and irradiated human foreskin fibroblasts and designed to minimize contact with xeno-components in knockout DMEM supplemented with knockout serum replacement, and basic fibroblast growth factor. Fingerprinting and HLA typing of the cell lines allowed their identification and traceability. Karyotype was normal for VAL-3 (46XY), VAL-4 (46XX) and VAL-5 (46XX). All three hESC l…

MaleHomeobox protein NANOGCellular differentiationTransplantation HeterologousCell Culture TechniquesGene ExpressionMice SCIDGerm layerBiologyCriptoCulture Media Serum-FreeCell LineMiceSOX2Mice Inbred NODmedicineAnimalsHumansEmbryonic Stem CellsDNA PrimersCryopreservationGeneticsBase SequenceObstetrics and GynecologyCell DifferentiationFibroblastsEmbryonic stem cellMolecular biologyCoculture TechniquesTransplantationmedicine.anatomical_structureReproductive MedicineSpainKaryotypingembryonic structuresFemaleEndodermBiomarkersStem Cell TransplantationDevelopmental BiologyReproductive BioMedicine Online
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