6533b82bfe1ef96bd128d902
RESEARCH PRODUCT
Defining the genomic signature of totipotency and pluripotency during early human development.
David MontanerVerónica RuizMaria Eugenia PooAna ConesaDiana ValbuenaEva SanchezCarlos SimónPatricia Diaz-gimenoJoaquín DopazoAmparo Galánsubject
EmbryologyBlastomeresMicroarraysCellular differentiationGene ExpressionCell Fate DeterminationMolecular Cell BiologyGene Regulatory NetworksInduced pluripotent stem cellreproductive and urinary physiologyGeneticsMultidisciplinarySystems BiologyStem CellsQTotipotentRGenomic signatureCell DifferentiationGenomicsCell biologyFunctional GenomicsBlastocyst Inner Cell MassBlastocyst Inner Cell Massembryonic structuresMedicineResearch ArticlePluripotent Stem CellsSystems biologyCell PotencyScienceEmbryonic DevelopmentBiologyMolecular GeneticsGeneticsHumansGene NetworksBiologyEmbryonic Stem CellsGenome HumanGene Expression ProfilingBio-OntologiesComputational BiologyMolecular Sequence AnnotationComparative GenomicsMolecular DevelopmentEmbryonic stem cellSignalingSignaling NetworksGene expression profilingGenome Expression AnalysisTotipotent Stem CellsDevelopmental Biologydescription
The genetic mechanisms governing human pre-implantation embryo development and the in vitro counterparts, human embryonic stem cells (hESCs), still remain incomplete. Previous global genome studies demonstrated that totipotent blastomeres from day-3 human embryos and pluripotent inner cell masses (ICMs) from blastocysts, display unique and differing transcriptomes. Nevertheless, comparative gene expression analysis has revealed that no significant differences exist between hESCs derived from blastomeres versus those obtained from ICMs, suggesting that pluripotent hESCs involve a new developmental progression. To understand early human stages evolution, we developed an undifferentiation network signature (UNS) and applied it to a differential gene expression profile between single blastomeres from day-3 embryos, ICMs and hESCs. This allowed us to establish a unique signature composed of highly interconnected genes characteristic of totipotency (61 genes), in vivo pluripotency (20 genes), and in vitro pluripotency (107 genes), and which are also proprietary according to functional analysis. This systems biology approach has led to an improved understanding of the molecular and signaling processes governing human pre-implantation embryo development, as well as enabling us to comprehend how hESCs might adapt to in vitro culture conditions.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2013-04-01 | PLoS ONE |