Dynamik des Ketonkörperstoffwechsels bei gesunden Ratten

Bis zu 150 μMol Acetacetat/kg/min besteht bei gesunden Ratten eine lineare Beziehung zwischen Infusionsrate und Blutspiegelanstieg an Ketonkorpern. Zunahme der Infusionsrate um 10 μMol Acetacetat/kg/min bewirkt einen Blutspiegelanstieg um 0,072 μMol Gesamtketonkorper/ml. Die totale Clearance fur Gesamtketonkorper betragt 139±17 ml · kg−1 · min−1. Uberschreitet die Zufuhr 150 μMol · kg−1 · min−1, so steigen die Ketonkorperspiegel rasch auf uberhohte Werte an. Aus den Daten lassen sich endogene Produktion und Verwertung von Ketonkorpern fur jeden stationaren Blutspiegel innerhalb der Umsatzkapazitat berechnen.

The effect of arabinofuranosyl-cytosine upon the synthesis of herpesvirus hominis Electron microscopic observations in relation to viral DNA-synthesis

The paper describes experiments about the degree of dependency of capsid, envelope and antigen synthesis byherpesvirus hominis upon viral DNA synthesis. The DNA synthesis has been blocked by different doses of Ara-C and the remnant DNA-synthesis has been measured by [3H]-thymidine incorporation after CsCl-density gradient centrifugation. Electron microscopic studies were done in parallel after incubation of infected cells with different doses of Ara-C. Finally, antigens were prepared after infection without and with added Ara-C. Increasing amounts of Ara-C inhibited the synthesis of viral DNA and infective particles. 1.5 μg Ara-C reduced the remaining incorporation into DNA to about less th…

Biological activity of 2-phenylethanol and its derivatives

The biosynthesis of herpesvirus DNA in rabbit kidney cells is inhibited to 50% by PEA (2-Phenylethanol) at 0.65 mg PEA/ml. The inhibition of cellular DNA synthesis in uninfected cells by PEA is about twice as sensitive as that of viral DNA synthesis.

Hemmung von DNA-Synthese und Zellvermehrung durch Apurins�ure und Apyrimidins�ure

Apurinic acid (APA) and apyrimidinic acid (APyA) prepared from deoxyribonucleic acid (DNA) by removing either purine or pyrimidine bases have been reported to inhibit enzymatic DNA-synthesis in vitro (Heicke, 1970). The present paper deals with in vivo effects of APA and APyA on mammalian cells in tissue culture.

Biological Activity of the phenylethanol and its derivatives. Influence on isolated DNA nucleotidyltransferase and DNAase.

High-molecular-weight deoxyribonuclease fromVerongia aerophoba

�ber die Wirkung von Apurins�ure und Apyrimidins�ure auf die enzymatische Desoxyribonukleins�ure-(DNA-) Synthese in vitro

The effect of chemically modified deoxyribonucleic acid (DNA) on enzymatic DNA-synthesis in vitro has been investigated with analogues of DNA prepared without either purine bases (apurinic acid, APA) or pyrimidine bases (apyrimidinic acid, APyA). DNA-synthesis in vitro was studied with the deoxynucleotide polymerizing enzymes purified from calf thymus (replicative and terminal deoxynucleotidyltransferase respectively).

Influence of molecular weight of DNA on the determination of anti-DNA antibodies in systemic lupus erythematosus (SLE) sera by radioimmunoassay

Using a radioimmunoassay (RIA) based on the Farr technique with radioactively labeled 3-H-DNA for quantitative measurements of anti-DNA antibodies in sera of patients with systemic lupus erythematosus (SLE), the influence of molecular weight of DNA (ranging from 0.1 times 10-6 to 22.0 times 10-6 daltons) on binding and precipitation in this system has been investigated. Comparing our results with mathematical models it follows that one antibody molecule is fixed on the average to a statistical DNA segment of 2 times 10-6 to 4 times 10-6 daltons. Furthermore binding capacity of the DNA was found to be independent of the molecular weight, as demonstrated in a double label experiment using 14-…

Apurinic acid, a modified DNA with anticancer activity

Apurinsaure, ein purinfreies hochmolekulares Desoxyribonukleinsaure-Derivat, hemmt das Wachstum des menschlichen Kolontumors GW-77 und das amelanotsiche Melanom des Hamsters.