Protéomique Shotgun des graines de féveroles : vers l’identification de protéines associées à la résistance aux bruches

Le protéome des graines en développement de lignées recombinantes issues du croisement entre desgénotypes de féverole résistants et sensibles aux bruches a été étudié par l’approche shotgun. Cetravail a permis d’identifier 749 protéines au début du remplissage des graines, dont 80 sontdifférentiellement accumulées entre les lignées sensibles et résistantes aux bruches. En plus de fournirun premier aperçu des protéines présentes à ce stade clé du développement des graines chez laféverole, ces données ont fait émerger des protéines candidates pour améliorer la résistance desgraines aux bruches. Parmi les protéines préférentiellement accumulée dans les lignées résistantes estune glycoprotéine …

A holistic overview of the impact of sulfur deficiency in pea facing water deficit

We report on the interplay between water deficit and sulfur deficiency, two constraints that are increasingly faced by crops due to climate change and low-input agricultural practices. In particular, we aim at better understanding the role of sulfur nutrition in the trade-off between seed quality establishment and plant stress tolerance in pea (Pisum sativum L.), a grain legume crop which has a pivotal role to play in both agroecological and food transitions. Like other legumes, pea is able to accumulate large amounts of seed proteins even in the absence of nitrogen fertilizers thanks to its symbiosis with N2-fixing soil bacteria. In this study, we deprived pea plants (cv. Caméor) of sulfur…

X!TandemPipeline: a tool to manage sequence redundancy for protein inference and phosphosite identification

X!TandemPipeline is a software designed to perform protein inference and to manage redundancy in the results of phosphosite identification by database search. It provides the minimal list of proteins or phosphosites that are present in a set of samples using grouping algorithms based on the principle of parsimony. Regarding proteins, a two-level classification is performed, where groups gather proteins sharing at least one peptide and subgroups gather proteins that are not distinguishable according to the identified peptides. Regarding phosphosites, an innovative approach based on the concept of phosphoisland is used to gather overlapping phosphopeptides. The graphical interface of X!Tandem…

Proteome adaptations under contrasting soil phosphate regimes of Rhizophagus irregularis engaged in a common mycorrhizal network.

International audience; For many plants, their symbiosis with arbuscular mycorrhizal fungi plays a key role in the acquisition of mineral nutrients such as inorganic phosphate (Pi), in exchange for assimilated carbon. To study gene regulation and function in the symbiotic partners, we and others have used compartmented microcosms in which the extra-radical mycelium (ERM), responsible for mineral nutrient supply for the plants, was separated by fine nylon nets from the associated host roots and could be harvested and analysed in isolation. Here, we used such a model system to perform a quantitative comparative protein profiling of the ERM of Rhizophagus irregularis BEG75, forming a common my…

Des potentiels régulateurs des réponses au stress hydrique et à la carence en soufre identifiés grâce à une analyse multi-omiques chez le pois

Dans leur environnement naturel, les plantes doivent faire face à plusieurs stress biotiqueset abiotiques au cours de leur cycle de développement. Certains de ces stress peuventsurvenir au même moment – comme le stress hydrique et les carences nutritionnelles – etpeuvent avoir des effets synergiques, antagonistes ou additifs sur les réponses moléculairesdes plantes. Chez le pois (Pisum sativum), il a été observé que l’effet de la carence en soufre(S) sur la composition protéique des graines peut être atténué lorsque cette carence estcombinée à un stress hydrique (Henriet et al., 2019). Afin de mieux caractériser les réponsesmoléculaires du pois au stress hydrique et/ou à la carence en S, un…

Membrane proteomic of arbuscular mycorrhiza

Membrane proteomic analyses to reveal and identify arbuscular mycorrhizal-related proteins

Multi-omics network analysis identifies putative regulators of molecular responses to water stress and sulfur deficiency in Pisum sativum

Plants in their physical environment face multiple biotic and abiotic stresses duringtheir life cycle. In nature, environmental stresses often co-occur – such as water deficit andnutrient deficiencies – and can have synergistic, antagonistic or additive effects on the plantmolecular responses. In pea (Pisum sativum), combination of water stress (WS) and sulfur (S)deficiency showed a mitigation effect on the seed protein composition, as compared to Sdeficiency occurring alone (Henriet et al., 2019). To better understand how pea responds toWS and/or S deficiency, a multi-omics (transcriptomics, proteomics, metabolomics, ionomics)analysis has been performed from leaf samples collected during a…

The membrane proteome of Medicago truncatula roots displays qualitative and quantitative changes in response to arbuscular mycorrhizal symbiosis

International audience; Arbuscular mycorrhizal (AM) symbiosis that associates roots of most land plants with soil-borne fungi (Glomeromycota), is characterized by reciprocal nutritional benefits. Fungal colonization of plant roots induces massive changes in cortical cells where the fungus differentiates an arbuscule, which drives proliferation of the plasma membrane. Despite the recognized importance of membrane proteins in sustaining AM symbiosis, the root microsomal proteome elicited upon mycorrhiza still remains to be explored. In this study, we first examined the qualitative composition of the root membrane proteome of Medicago truncatula after microsome enrichment and subsequent in dep…

Etude du rôle de la nutrition soufrée dans la réponse du pois au stress hydrique: vers la construction de réseaux protéiques

International audience

Protéome membranaire en réponse à la symbiose mycorhizienne à arbuscules par GeLC-MS/MS

Studying the interplay between sulfur nutrition and water stress tolerance in pea by proteomics : a focus on seed development and composition

International audience; Water stress and sulfur-deficiency are two constraints increasingly faced by crops due to climatechange and low-input practices. To investigate their interplay in the grain legume pea (Pisum sativumL.), sulfate was depleted at mid-vegetative stage and a moderate 9-day water stress period was imposedduring the early reproductive phase. The combined stress accelerated seed production, lowering yield,one-seed weight and seed number per plant, but rebalanced seed protein composition. In fact, themoderate water stress mitigated the negative effect of sulfur-deficiency on the accumulation of sulfurrichproteins in seeds, probably due to a lower seed sink strength for nitrog…

Mycorrhization-induced changes in the root plastid proteome of Medicago truncatula

How does sulfur deficiency modulate pea response to water stress? Impact on early developing and mature seeds

National audience; Pea (Pisum sativum L.) produces seeds rich in proteins but yield and quality remain unstable across years due to various stresses. Sulfur (S) deficiency and water stress are two abiotic stresses that interact in the current context of climate change and low-input practices, and recent studies suggest an important role for sulfate transport and metabolism in the plant response to water stress. To investigate the interplay between sulfur nutrition and the water stress response, sulfate-deprived pea plants were subjected to a 9-days period of water stress during the early reproductive phase. While water stress did not impact seed yield, sulfur deficiency alone or combined wi…

Changes in the pea seed proteome in response to drought combined with sulfur deficiency

EABAPGEAPSI DOCT INRA; Pea (Pisum sativum L.) produces seeds rich in proteins, but seed yield and quality remain unstable across years due to abiotic stresses occurring during the reproductive period. Drought and sulfur deficiency are two abiotic stresses that interact in the current context of climate change and lowinput practices, and recent studies suggest a role of sulfate transport and metabolism in the plant response to drought (Ernst et al., 2010; Chan et al., 2013; Gallardo et al., 2014; Ahmad et al., 2016). In this study, we investigated the impact of sulfur deficiency combined with drought on the pea seed proteome. Pea plants were subjected to sulfur-deficiency two weeks after sow…

Label-free 1-DE-LC-MS/MS and iTRAQ-OFFGEL-LC-MS/MS to identify arbuscular mycorrhiza-related membrane proteins.

Analyse protéomique des graines de pois en embryogénèse et à maturité : Impact d’un stress hydrique combiné à une carence en soufre

International audience

L'interaction entre la nutrition soufrée et la réponse du pois au stress hydrique

International audience

Vers l'étude quantitative et fonctionnelle des protéomes membranaires des racines mis en jeu au cours de la symbiose mycorhizienne à arbuscules de Medicago truncatula

Etude du rôle de la nutrition soufrée dans la réponse du pois au stress hydrique par une approche protéomique : Focus sur les graines

International audience

Label-free 1-DE-LC-MS/MS to identify arbuscular mycorrhiza-related membrane proteins

Deep changes in the shape and number of organelles, together with profound modifications in various membrane compartments, are induced within arbuscular mycorrhizal (AM) symbiosis. In this context, to investigate the membrane-associated proteins that are regulated in the model interaction Medicago truncatula – Rhizophagus irregularis, label-free 1DE-LC-MS/MS approach has been employed as alternative to two-dimensional gel electrophoresis. The existence of a correlation between protein abundance and peak areas or number of MS/MS spectra has widened the choice of label-free quantitative proteomics. The results highlighted microsomal protein candidates that could be involved in the symbiotic e…