0000000001198003
AUTHOR
Karine Gallardo-guerrero
Storage cells – oil and protein bodies
BAPGEAPSI; Molecular Cell Biology of the Growth and Differentiation of Plant Cells encompasses cell division, cell enlargement and differentiation; which is the cellular basis of plant growth and development. Understanding these developmental processes is fundamental for improving plant growth and the production of special plant products, as well as contributing to biological understanding. The dynamics of cells and cellular organelles are considered in the context of growth and differentiation, made possible particularly by advances in molecular genetics and the visualization of organelles using molecular probes. There is now a much clearer understanding of these basic plant processes of c…
Optimizing response to abiotic stress
International audience
The role of sulfur in the control of seed quality traits in legumes: from model to crop species
National audience; voir pdf
Regulation of legume seed size by an endosperm-expressed transcription factor
BAP Pôle GEAPSI; International audience; There are numerous reports of transcription factors (TFs) which are implicated in the control of seed size and seed composition. We have identified, using a platform of TF sequences derived from the Medicago truncatula genome sequence, a class of TFs specifically expressed during the seed filling stage. One such TF, DASH, was shown to be confined to the developing endosperm. We investigated the role played by DASH through analysis of mutant alleles. These give rise to seed-lethal or near-lethal phenotypes, with degeneration of the endosperm and arrested embryo development. The relation of this phenotype to seed auxin action was investigated.
Genetic diversity for seed protein composition in Meticago truncatula
International audience
How does sulfur deficiency modulate pea response to water stress? Impact on early developing and mature seeds
National audience; Pea (Pisum sativum L.) produces seeds rich in proteins but yield and quality remain unstable across years due to various stresses. Sulfur (S) deficiency and water stress are two abiotic stresses that interact in the current context of climate change and low-input practices, and recent studies suggest an important role for sulfate transport and metabolism in the plant response to water stress. To investigate the interplay between sulfur nutrition and the water stress response, sulfate-deprived pea plants were subjected to a 9-days period of water stress during the early reproductive phase. While water stress did not impact seed yield, sulfur deficiency alone or combined wi…
Changes in the pea seed proteome in response to drought combined with sulfur deficiency
EABAPGEAPSI DOCT INRA; Pea (Pisum sativum L.) produces seeds rich in proteins, but seed yield and quality remain unstable across years due to abiotic stresses occurring during the reproductive period. Drought and sulfur deficiency are two abiotic stresses that interact in the current context of climate change and lowinput practices, and recent studies suggest a role of sulfate transport and metabolism in the plant response to drought (Ernst et al., 2010; Chan et al., 2013; Gallardo et al., 2014; Ahmad et al., 2016). In this study, we investigated the impact of sulfur deficiency combined with drought on the pea seed proteome. Pea plants were subjected to sulfur-deficiency two weeks after sow…
Adapter la composition protéique des graines de légumineuses en fonction des usages
National audience
L'accumulation des protéines dans les graines de légumineuses
The agroecological benefits of legumes and the renewed interest in the consumption of vegetable proteins stimulate researches on cognitive and applied aspects of seed biology aiming at optimizing protein composition of legume seeds. This article presents the state of knowledge on the genetic and environmental control of the accumulation of major proteins found in legume seeds, with a view to developing varieties with improved nutritional seed quality. It also shows how high-throughput genomics and post-genomics data, along with translational research aiming at transferring knowledge between model and crop species, can accelerate the identification of genes controlling the accumulation of st…
Legume adaptation to sulfur deficiency
BAPSPEEAGEAPSIIPM; Because sulfur-deficiency represents an agronomic issue for crops and notably for the quality of legume products, it is necessary to provide sulfur fertilizers according to crop sulfur needs along with improving sulfur use efficiency. To achieve this goal, increased knowledge of how plants respond to sulfur-deficiency is required. We subjected Medicago truncatula, a model legume species, to sulfur deficiency at different developmental stages and compared yield, nutrient allocation and seed traits. We revealed an impressive plasticity of M. truncatula that varied according to the S-stress period and influenced seed composition and germination. Sulfur deficiency at a mid-ve…