0000000001316390
AUTHOR
Lucile Dondaine
Lipoproteins LDL versus HDL as nanocarriers to target either cancer cells or macrophages
free open access article 31 p.; International audience; In this work, we have explored natural unmodified low- and high-density lipoproteins (LDL and HDL) as selective delivery vectors in colorectal cancer therapy. We show in vitro in cultured cells and in vivo (NanoSPECT/CT) in the CT-26 mice colorectal cancer model that LDLs are mainly taken up by cancer cells, while HDLs are preferentially taken up by macrophages. We loaded LDLs with cisplatin and HDLs with the heat shock protein-70 inhibitor AC1LINNC, turning them into a pair of “Trojan horses” delivering drugs selectively to their target cells as demonstrated in vitro in human colorectal cancer cells and macrophages, and in vivo. Coupl…
Nanofitins targeting heat shock protein 110: an innovative immunotherapeutic modality in cancer.
The presence of an inactivating heat shock protein 110 (HSP110) mutation in colorectal cancers has been correlated with an excellent prognosis and with the ability of HSP110 to favor the formation of tolerogenic (M2-like) macrophages. These clinical and experimental results suggest a potentially powerful new strategy against colorectal cancer: the inhibition of HSP110. In this work, as an alternative to neutralizing antibodies, Nanofitins (scaffold ~7 kDa proteins) targeting HSP110 were isolated from the screening of a synthetic Nanofitin library, and their capacity to bind (immunoprecipitation, biolayer interferometry) and to inhibit HSP110 was analyzed in vitro and in vivo. Three Nanofiti…
Coumarin-Phosphine-Based Smart Probes for Tracking Biologically Relevant Metal Complexes: From Theoretical to Biological Investigations
International audience; Ten metal-based complexes and associated ligands have been synthesized and characterized. One of the metal ligands is a coumarin-phosphine derivative, which displays tunable fluorescence properties. The fluorescence is quenched in the case of the free ligand and ruthenium and osmium complexes, whereas it is strong for the gold complexes and phosphonium derivatives. These trends were rationalized by theoretical calculations, which revealed non-radiative channels involving a dark state for the free ligands that is lower in energy than the emissive state and is responsible for the quenching of fluorescence. For the Ru-II and Os-II complexes, other non-radiative channels…
Highly antiproliferative neutral Ru(ii)-arene phosphine complexes
Six ruthenium(II)- and four gold(I)-phosphine based complexes were synthesized and fully characterized. Some of them displayed strong antiproliferative properties for several types of cancer including colon, breast, and lung. Notably, two of the Ru(II) complexes displayed an IC50 of around 2 μM, which is exceptional for these types of complexes. The dramatic impact of the nature of the arene coordinated on the ruthenium center was clearly evidenced.
Anticancer Agents: Does a Phosphonium Behave Like a Gold(I) Phosphine Complex? Let a “Smart” Probe Answer!
Gold phosphine complexes, such as auranofin, have been recognized for decades as antirheumatic agents. Clinical trials are now underway to validate their use in anticancer or anti-HIV treatments. However, their mechanisms of action remain unclear. A challenging question is whether the gold phosphine complex is a prodrug that is administered in an inactive precursor form or rather that the gold atom remains attached to the phosphine ligand during treatment. In this study, we present two novel gold complexes, which we compared to auranofin and to their phosphonium analogue. The chosen ligand is a phosphine-based smart probe, whose strong fluorescence depends on the presence of the gold atom. …
Gold( i )–BODIPY–imidazole bimetallic complexes as new potential anti-inflammatory and anticancer trackable agents
International audience; Two new gold(I)–BODIPY–imidazole based trackable therapeutic bimetallic complexes have been synthesized and fully characterized. They display strong antiproliferative properties on several types of cancers including colon, breast, and prostate and one of them presents a significant anti-inflammatory effect. Additionally, the two compounds could be visualised in vitro by confocal microscopy in the submicromolar range.
CCDC 1420553: Experimental Crystal Structure Determination
Related Article: Lucile Dondaine, Daniel Escudero, Moussa Ali, Philippe Richard, Franck Denat, Ali Bettaieb, Pierre Le Gendre, Catherine Paul, Denis Jacquemin, Christine Goze and Ewen Bodio|2016|Eur.J.Inorg.Chem.||545|doi:10.1002/ejic.201501304
CCDC 1014822: Experimental Crystal Structure Determination
Related Article: Moussa Ali , Lucile Dondaine , Anais Adolle , Carla Sampaio , Florian Chotard , Philippe Richard , Franck Denat , Ali Bettaieb , Pierre Le Gendre , Véronique Laurens , Christine Goze , Catherine Paul , and Ewen Bodio|2015|J.Med.Chem.|58|4521|doi:10.1021/acs.jmedchem.5b00480
CCDC 1538131: Experimental Crystal Structure Determination
Related Article: Audrey Trommenschlager, Florian Chotard, Benoît Bertrand, Souheila Amor, Lucile Dondaine, Michel Picquet, Philippe Richard, Ali Bettaïeb, Pierre Le Gendre, Catherine Paul, Christine Goze, Ewen Bodio|2017|Dalton Trans.|46|8051|doi:10.1039/C7DT01377A
CCDC 1014823: Experimental Crystal Structure Determination
Related Article: Moussa Ali , Lucile Dondaine , Anais Adolle , Carla Sampaio , Florian Chotard , Philippe Richard , Franck Denat , Ali Bettaieb , Pierre Le Gendre , Véronique Laurens , Christine Goze , Catherine Paul , and Ewen Bodio|2015|J.Med.Chem.|58|4521|doi:10.1021/acs.jmedchem.5b00480
CCDC 1420552: Experimental Crystal Structure Determination
Related Article: Lucile Dondaine, Daniel Escudero, Moussa Ali, Philippe Richard, Franck Denat, Ali Bettaieb, Pierre Le Gendre, Catherine Paul, Denis Jacquemin, Christine Goze and Ewen Bodio|2016|Eur.J.Inorg.Chem.||545|doi:10.1002/ejic.201501304
CCDC 1014821: Experimental Crystal Structure Determination
Related Article: Moussa Ali , Lucile Dondaine , Anais Adolle , Carla Sampaio , Florian Chotard , Philippe Richard , Franck Denat , Ali Bettaieb , Pierre Le Gendre , Véronique Laurens , Christine Goze , Catherine Paul , and Ewen Bodio|2015|J.Med.Chem.|58|4521|doi:10.1021/acs.jmedchem.5b00480