Epigastric cutaneous discharge: think amoebiasis

Évaluation du kit de PCR en temps réel MycoGENIE ® Pneumocystis jirovecii pour le diagnostic moléculaire de la pneumocystose

Pneumocystis jirovecii (Pj) est un champignon pathogene opportuniste strictement humain. Il colonise transitoirement et sans symptome l’arbre respiratoire des patients immunocompetents, et peut etre responsable d’infections pulmonaires severes chez les patients immunodeprimes. Le diagnostic biologique de pneumocystose repose sur la mise en evidence du pathogene a l’examen direct d’un prelevement pulmonaire profond. Si l’examen direct manque souvent de sensibilite, de nombreuses techniques de PCR se sont developpees pour ameliorer la sensibilite de detection du champignon. Les techniques de PCR quantitatives (qPCR) permettent de quantifier la charge fongique dans le prelevement, et potentiel…

Oestrus ovis external ophtalmomyiasis : a case report in Burgundy France

Background External ophtalmomyiasis (EOM) is a zoonosis related to the presence of Oestrus ovis larvae at the ocular level in small ruminants (i.e. ovine, caprine). In humans, EOM is a rare cosmopolitan disorder, mostly described in warm and dry rural areas in patients living close to livestock areas. In metropolitan France (excluding Corsica), EOM is an exceptional disease with less than 25 cases recorded since 1917. Case presentation We report a case of EOM in a 19-years old man in the last week of September 2016 in Burgundy. Conclusion The diagnosis of an EOM in Burgundy, a French region described as cold and humid, is surprising and could be due to a more marked climatic warming during …

Profil épidémiologique et mycologique des dermatophytoses au CHU de Dijon (2007–2016)

International audience; IntroductionL’objectif de ce travail a été d’étudier le profil épidémiologique et mycologique des dermatophytoses diagnostiquées au laboratoire de parasitologie-mycologie du CHU de Dijon.Matériel et méthodesIl s’agit d’une étude rétrospective qui couvre une période de 10 ans (2007–2016). Seuls les examens mycologiques positifs pour les dermatophytes ont été pris en compte pour cette étude (genres Epidermophyton, Microsporum et Trichophyton).De janvier 2007 à août 2014, l’identification de ces micromycètes a été réalisée selon les caractères macro- et microscopiques des colonies fongiques. À partir de septembre 2014, leur identification a été réalisée par microscopie …

Tight Junctions as a Key for Pathogens Invasion in Intestinal Epithelial Cells

Tight junctions play a major role in maintaining the integrity and impermeability of the intestinal barrier. As such, they act as an ideal target for pathogens to promote their translocation through the intestinal mucosa and invade their host. Different strategies are used by pathogens, aimed at directly destabilizing the junctional network or modulating the different signaling pathways involved in the modulation of these junctions. After a brief presentation of the organization and modulation of tight junctions, we provide the state of the art of the molecular mechanisms leading to permeability breakdown of the gut barrier as a consequence of tight junctions’ attack by pathogens, including…

Additional file 1: of Oestrus ovis external ophtalmomyiasis: a case report in Burgundy France

Figure S1. Parasite cycle of Oestrus ovis. Oestrus ovis exerts a strict parasitism of the nasal cavities of small sheep and goat ruminants. The viviparous females of Oestrus ovis deposit first-stage larvae (L1) directly in the nasal orifices of sheep and goats. L1 actively penetrate through the nasal orifices and colonize the cornets and septum where they will develop. Once located at the ethmoid level, L1 molt to stage 2 larvae (L2). L2 further ascend from the nasal cavity to the frontal sinuses where they molt to stage 3 larvae (L3). Thereafter, L3 are expelled from the nasal cavity of the host by sneezing via the nasal mucus that subsequently contaminate the soils. Then, L3 turn into a p…