6533b7cefe1ef96bd12578e2

RESEARCH PRODUCT

Brominated Azaphilones from the Sponge-Associated Fungus Penicillium canescens Strain 4.14.6a

Attila MándiTibor KurtánMarian FrankFerhat Can ÖZkayaWenhan LinZhen LiuMalte PlenkerAlexandra HamacherMatthias U. KassackPeter ProkschRudolf HartmannWerner E.g. Müller

subject

PharmacologyBicyclic molecule010405 organic chemistryStereochemistryOrganic ChemistryAbsolute configurationPharmaceutical ScienceEther01 natural sciences0104 chemical sciencesAnalytical Chemistry010404 medicinal & biomolecular chemistryPigmentchemistry.chemical_compoundComplementary and alternative medicinechemistryPenicillium canescensvisual_artDrug Discoveryvisual_art.visual_art_mediumMolecular MedicineMoietyFermentationMethylene

description

The fungus Penicillium canescens was isolated from the inner tissue of the Mediterranian sponge Agelas oroides. Fermentation of the fungus on solid rice medium yielded one new chlorinated diphenyl ether (1) and 13 known compounds (2-14). Addition of 5% NaBr to the rice medium increased the amounts of 4-6, while lowering the amounts of 8, 12, and 14. Furthermore, it induced the accumulation of 17 and two new brominated azaphilones, bromophilones A and B (15 and 16). Compounds 15 and 16 are the first example of azaphilones with the connection of a benzene moiety and the pyranoquinone core through a methylene group. The structures of the new compounds were elucidated based on the 1D and 2D NMR spectra as well as on HRESIMS data. The absolute configuration of the condensed bicyclic moiety of 15 and 16 was determined by sTDA ECD calculations. Compound 16 exhibited moderate cytotoxicity against the mouse lymphoma cell line L5178Y (IC50 8.9 μM), as well as against the human ovarian cancer cell line A2780 (IC50 2.7 μM), whereas the stereoisomer 15 was considerably less active.

yearjournalcountryeditionlanguage
2019-07-30Journal of Natural Products
https://doi.org/10.1021/acs.jnatprod.9b00151