6533b7d0fe1ef96bd125ae9d
RESEARCH PRODUCT
Isocratic high-performance liquid chromatographic determination of tryptophan in infant formulas.
J.c. LópezRosaura FarréM. FerrerésReyes BarberáAmparo AlegríaMaría Jesús Lagardasubject
AcetatesBiochemistryHigh-performance liquid chromatographySensitivity and SpecificityAnalytical ChemistryBarium hydroxidechemistry.chemical_compoundColumn chromatographyIsothiocyanatesHumansDerivatizationChromatography High Pressure LiquidAcetic AcidDetection limitChromatographyPhenyl isothiocyanateElutionOrganic ChemistryOsmolar ConcentrationTryptophanInfant NewbornTryptophanInfantGeneral MedicineHydrogen-Ion ConcentrationchemistrySolventsInfant FoodThiocyanatesdescription
The application to infant formulas of a method for tryptophan determination by isocratic HPLC with UV detection at 254 nm, after derivatization with phenyl isothiocyanate, was studied. Protein was hydrolysed by barium hydroxide at 120 degrees C for 8 h, followed by derivatization with phenyl isothiocyanate, HPLC and UV detection at 254 nm. The optimum chromatographic conditions (pH, ionic strength of elution solvent and eluent ratio) were established. The analytical parameters (linearity, precision, accuracy of derivatization and limits of detection and quantification) were determined. The values obtained demonstrated that the method is useful for determining the tryptophan content of infant formulas. The tryptophan contents in six different types of infant formulas were 0.535-0.848 g per 100 g of protein.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1996-01-15 | Journal of chromatography. A |