6533b7d0fe1ef96bd125b980
RESEARCH PRODUCT
Visualizing a protein quake with time-resolved X-ray scattering at a free-electron laser
Daniel P. DeponteMichael J. BoganIrina KoshelevaDaniel JamesRobert HenningFrancesco StellatoAndrew AquilaKasper S. KjærKasper S. KjærErik MalmerbergIlme SchlichtingJohn C. H. SpenceChristopher KupitzM. Marvin SeibertJennie SjöhamnAndrew V. MartinThomas A. WhiteMarc MesserschmidtCecilia WickstrandDingjie WangHenry N. ChapmanRichard A. KirianGergely KatonaRaimund FrommeDavid ArnlundPeter BerntsenJan DavidssonTim Brandt Van DrielNadia A. ZatsepinGerrit GroenhofGarth J. WilliamsLinda C. JohanssonDespina MilathianakiSadia BariMark S. HunterSébastien BoutetSebastian WestenhoffUwe WeierstallR. Bruce DoakMatthias FrankMartin NielsenMengning LiangIngo GrotjohannRobert L. ShoemanPetra FrommeRichard NeutzeAnton Bartysubject
Photosynthetic reaction centreMaterials scienceProtein ConformationPhysics::OpticsPhycobiliproteinsfrequency vibrational-modesRadiation DosageBiochemistryMolecular physicsArticlelaw.inventionProtein structureX-Ray Diffractionlawddc:570Scattering Small AngleMolecular Biologyta116Quantitative Biology::BiomoleculesScatteringLasersMolecular biophysicsFree-electron laserCell BiologyLaserstructural dynamicsEnergy TransferPicosecondBiophysicsUltrashort pulseBiotechnologydescription
We describe a method to measure ultrafast protein structural changes using time-resolved wide-angle X-ray scattering at an X-ray free-electron laser. We demonstrated this approach using multiphoton excitation of the Blastochloris viridis photosynthetic reaction center, observing an ultrafast global conformational change that arises within picoseconds and precedes the propagation of heat through the protein. This provides direct structural evidence for a 'protein quake': the hypothesis that proteins rapidly dissipate energy through quake-like structural motions. peerReviewed
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2014-08-10 | Nature methods |