6533b7d1fe1ef96bd125cd37
RESEARCH PRODUCT
Evaluation and comparison of tailor-made stationary phases based on spherical silica-based beads for capillary electrochromatography via peptide separation analysis.
T.p. HennessyK.k. UngerDieter LubdaM I Hubersubject
chemistry.chemical_classificationAnalyteCapillary electrochromatographyChromatographySilicon dioxideClinical BiochemistryAnalytical chemistryCell BiologyGeneral MedicineElectrolyteSilicon DioxideBiochemistryCyclic peptideAnalytical Chemistrychemistry.chemical_compoundchemistryElectrochromatographycardiovascular systemSurface modificationAmino Acid SequencePorosityPeptidesChromatography Micellar Electrokinetic Capillarydescription
Small cyclic peptides have been employed to elucidate the performance of novel sorbents as stationary phases in capillary electrochromatography (CEC). In this paper chain length dependencies for ordinary liquid chromatographic sorbents are reported together with findings acquired on beads specifically designed to suit CEC. The latter, tailor-made, spherical, porous silica exhibits a distinguished surface modification to meet the criteria anticipated to enhance performance profiles in CEC. With well-characterised peptides resembling the analytes, probing of the CEC system in a systematic manner (predominantly via the organic modifier content of the background electrolyte (BE)) reveals insight into the complex interplay occurring in such analytical systems at the molecular and sub-molecular level in particular upon various modes of interaction.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2004-04-15 | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences |