6533b7d5fe1ef96bd1263ee9
RESEARCH PRODUCT
Immuno-MALDI-MS in Human Plasma and on-Chip Biomarker Characterizations at the Femtomole Level
Patrick DucoroyMarven El OstaGé Raldine LucchiAlain RouleauWilfrid Boireausubject
immuno MALDI-MSMaldi mseducationlcsh:Chemical technologyProteomicsMass spectrometry01 natural sciencesBiochemistryArticleAnalytical Chemistry[SPI.MAT]Engineering Sciences [physics]/MaterialsAutomation03 medical and health sciencesproteomicsLimit of DetectionLab-On-A-Chip DevicesHumanslcsh:TP1-1185Electrical and Electronic Engineering[SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/MicroelectronicsBiochipInstrumentationmass spectrometry030304 developmental biologyDetection limit[SPI.ACOU]Engineering Sciences [physics]/Acoustics [physics.class-ph]0303 health sciencesChromatographyChemistry010401 analytical chemistryReproducibility of ResultsSurface Plasmon ResonanceAtomic and Molecular Physics and Optics0104 chemical sciencesBiomarker (cell)Human plasmaSpectrometry Mass Matrix-Assisted Laser Desorption-IonizationSurface Plasmon Resonance; mass spectrometry; immuno MALDI-MS; biomarker; proteomicsbiomarkerBiosensorBiomarkersdescription
Immuno-SPR-MS is the combination of immuno-sensors in biochip format with mass spectrometry. This association of instrumentation allows the detection and the quantification of proteins of interest by SPR and their molecular characterization by additional MS analysis. However, two major bottlenecks must be overcome for a wide diffusion of the SPR-MS analytical platform: (i) To warrant all the potentialities of MS, an enzymatic digestion step must be developed taking into account the spot formats on the biochip and (ii) the biological relevancy of such an analytical solution requires that biosensing must be performed in complex media. In this study, we developed a procedure for the detection and the characterization at ~1 µg/mL of the LAG3 protein spiked in human plasma. The analytical performances of this new method was established, particularly its specificity (S/N > 9) and sensitivity (100% of LAG3 identification with high significant mascot score >68 at the femtomole level). The collective and automated on-chip MALDI-MS imaging and analysis based on peptidic fragments opens numerous applications in the fields of proteomics and diagnosis.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2012-11-06 |