6533b7d5fe1ef96bd1264947
RESEARCH PRODUCT
Direct identification of microorganisms from thioglycolate broth by MALDI-TOF MS.
Manuel Rodríguez-iglesiasJorge Arca-suárezPilar Marín-casanovaFátima Galán-sánchezsubject
0301 basic medicinePhysiologyMicroorganismlcsh:MedicinePathology and Laboratory MedicineNervous SystemMass SpectrometryAnalytical Chemistrychemistry.chemical_compoundSpectrum Analysis TechniquesMicrobial PhysiologyCandida albicansMedicine and Health SciencesMatrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometrylcsh:ScienceCerebrospinal FluidMultidisciplinaryChemistryMicrobial Growth and DevelopmentClinical Laboratory SciencesBody FluidsClinical microbiologyChemistryClinical LaboratoriesThioglycolatesPhysical SciencesAnaerobic bacteriaAnatomyCellular DebrisResearch ArticleClinical PathologyThioglycolate broth030106 microbiologyAnaerobic BacteriaResearch and Analysis MethodsMicrobiology03 medical and health sciencesSigns and SymptomsDiagnostic MedicineGram Negative BacteriaLaboratory methodsChromatographyBacteriaBacterial Growthlcsh:ROrganismsBiology and Life SciencesBacteriologyCulture MediaMatrix-assisted laser desorption/ionizationAbscessesClinical MicrobiologySpectrometry Mass Matrix-Assisted Laser Desorption-Ionizationlcsh:QLaboratoriesDevelopmental Biologydescription
We developed an easy MALDI-TOF MS-based assay to identify microorganisms directly from thioglycolate broth. A total of 101 positive thioglycolate broths inoculated with 15 different kinds of samples were evaluated. In 91 samples (90.1%), direct MALDI-TOF MS identifications were the same as those obtained after conventional laboratory procedures including subcultures. In 10 samples misidentified by direct processing, yeasts or mixed cultures grew in the thioglycolate subcultures, or high cellular debris hampered a correct analysis. This rapid method can provide a fast, clinically- relevant species-level identification without disturbing the daily workflow in clinical microbiology laboratories.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2017-09-21 | PLoS ONE |