6533b7d8fe1ef96bd126a429
RESEARCH PRODUCT
Assessment of Tumor Development and Wound Healing Using Endoscopic Techniques in Mice
Nadine WittkopfMarkus F. NeurathAlexandra WlodarskiClemens NeufertChristoph BeckerMaximilian J. WaldnerClaudia GüntherStefan Wirtzsubject
Pathologymedicine.medical_specialtyColorectal cancerBiopsyDiseasePathogenesisMiceMicromanipulation03 medical and health sciences0302 clinical medicineIn vivoIntestinal inflammationmedicineAnimalsHumans030304 developmental biologyWound Healing0303 health sciencesMiniaturizationHepatologymedicine.diagnostic_testbusiness.industryDisease mechanismsGastroenterologyEndoscopyColitismedicine.disease3. Good healthEndoscopyEndoscopes GastrointestinalDisease Models AnimalColonic Neoplasms030211 gastroenterology & hepatologybusinessWound healingdescription
Mouse models of intestinal inflammation and colon cancer are valuable tools to gain insights into the pathogenesis of the corresponding human diseases. Recently, in vivo mouse endoscopy has been developed, allowing not only the high-resolution monitoring and scoring of experimental disease development, but also enables the investigator to perform manipulations, including local injection of reagents or the taking of biopsies for molecular and histopathologic analyses. Chromoendoscopic staining with methylene blue enables visualization of the crypt structure and allows discrimination between inflammatory and neoplastic changes. The development of endoscopic techniques in live mice opened new options for the investigation of disease mechanisms in the gut and for the preclinical testing of potential therapeutic effects of drug candidates. Finally, mouse endoscopy can help to reduce animal numbers needed to gain significant experimental data.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2010-10-20 | Gastroenterology |