6533b7d9fe1ef96bd126cb54
RESEARCH PRODUCT
Determination of amobarbital and secobarbital in plasma samples using micellar liquid chromatography
Rosa María Villanueva-camañasYolanda Martín-bioscaM.j. Medina-hernándezSalvador SagradoC. Quiñones‐torrelosubject
PharmacologyDetection limitChromatographyPlasma samplesChemistryButabarbitalAmobarbitalClinical BiochemistryAnalytical chemistryGeneral MedicineSecobarbitalBiochemistryAnalytical ChemistryDilutionMicellar liquid chromatographyDrug DiscoverymedicineUv detectionMolecular Biologymedicine.drugdescription
A new liquid chromatographic procedure for the determination of amobarbital and secobarbital in plasma samples is proposed. The method uses a Spherisorb octadecylsilane ODS-2 C18 analytical column, a guard column of similar characteristics and 0.04 M CTAB solution buffered at pH 7.5 containing 3% 1-propanol as micellar mobile phase. The UV detection was carried out at 250 nm. Butabarbital was used as internal standard. Plasma samples preparation only required adequate dilution with the mobile phase before injection into the chromatographic system. The limits of detection were 0.2 and 0.4 mg/L for amobarbital and secobarbital, respectively. The proposed method allows the determination of amobarbital and secobarbital in plasma at therapeutic levels. Copyright © 2000 John Wiley & Sons, Ltd.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2000-01-01 | Biomedical Chromatography |