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RESEARCH PRODUCT
Science based calibration for the extraction of 'analyte-specific' HPLC-DAD chromatograms in environmental analysis
M. De La GuardiaJulia KuligowskiHéctor C. GoicoecheaM. Martínez GaleraGuillermo QuintásM.d. Gil GarcíaSalvador GarriguesMaría J. Culzonisubject
AnalyteResolution (mass spectrometry)Adrenergic beta-AntagonistsStatistics as TopicAnalytical chemistryEnvironmentHigh-performance liquid chromatographyAnalytical ChemistryMatrix (chemical analysis)AbsorbanceRiversSCIENCE BASED CALIBRATIONSample preparationLeast-Squares AnalysisElectrodesChromatography High Pressure LiquidAnalgesicsENVIRONMENTAL ANALYSISChromatographyChemistryOtras Ciencias QuímicasExtraction (chemistry)Ciencias QuímicasDrug ResiduesStandard curveHIGH PERFORMANCE LIQUID CHROMATOGRAPHY-DIODE ARRAY DETECTION (HPLC-DAD)CalibrationMultivariate AnalysisEnvironmental PollutantsCIENCIAS NATURALES Y EXACTASdescription
Multivariate science based calibration (SBC) has been applied to the resolution of overlapped peaks in liquid chromatography with diode array detection (LC-DAD). Complex river water samples spiked with 11 pharmaceutical substances resulted in poorly resolved chromatograms containing additional peaks from interfering matrix compounds and a change in the background absorbance due to the mobile phase gradient. Applying the present multivariate approach it was possible to resolve all 11 analytes from overlapping peaks, obtaining linear calibration lines (R2 > 0.96). Recovery percentages on spiked samples ranged between 74.6 and 113.5%, which are quite satisfactory taking into account the low concentration ranges considered to 1-7 μg L-1. Fil: Kuligowski, J.. Universidad de Valencia; España Fil: Martínez Galera, M.. Universidad de Almería; España Fil: Gil García, M. D.. Universidad de Almería; España Fil: Culzoni, Maria Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina Fil: Garrigues, S.. Universidad de Valencia; España Fil: Quintás, G.. Universidad de Valencia; España Fil: de la Guardia, Miguel. Universidad de Valencia; España
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2010-04-15 |