6533b7ddfe1ef96bd1274a53
RESEARCH PRODUCT
Rare association of human herpesvirus 6 DNA with human papillomavirus DNA in cervical smears of women with normal and abnormal cytologies.
Maria Rosaria VillafrateF. M. RomanoFrancesco VitaleF BonuraNino RomanoEnza VivianoR GuttadauroAnna Maria Pernasubject
Microbiology (medical)AdultPathologymedicine.medical_specialtyvirusesHerpesvirus 6 HumanUterine Cervical NeoplasmsCervix UteriBiologyCervical intraepithelial neoplasiamedicine.disease_causePolymerase Chain ReactionHerpesviridaelaw.inventionlawmedicineHumansPapillomaviridaePapillomaviridaePolymerase chain reactionCervical cancerVaginal SmearsCocarcinogenesisvirus diseasesMiddle Agedmedicine.diseasebiology.organism_classificationUterine Cervical DysplasiaVirologyKoilocyteDNA ViralHuman herpesvirus 6FemaleNested polymerase chain reactionResearch Articledescription
We investigated by nested PCR the possible association of human herpesvirus 6 (HHV-6) and human papillomavirus (HPV) genomes in the cervixes of 109 women with normal and abnormal cytological smears. HPV DNA was detected in 8.33% of 24 women with normal cytologies and in 41.1% of 85 women with abnormal cytologies; the proportion of HPV DNA was directly related to the severity of the lesions. HHV-6 DNA was found in only one patient, who had a cytological pattern of koilocytosis. The HHV-6 genome was classified by restriction enzyme analysis as variant B. The study indicates that detection of the HHV-6 genome in the cervixes of women with a wide spectrum of gynecological complaints is a rare event and rules out the possible association between HHV-6 and HPV genomes in cervical cancer lesions.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1996-06-01 | Journal of clinical microbiology |