6533b7ddfe1ef96bd1275552
RESEARCH PRODUCT
Annotation of microsporidian genomes using transcriptional signals
Ivan WawrzyniakSébastien TerratOlivier GonçalvesNicolas ParisotNicolas ParisotPierre PeyretPatrick WinckerPatrick WinckerCorinne Biderre-petitSimone DupratFrédéric DelbacJean WeissenbachJean WeissenbachSébastien RimourEric Dugat-bonyAntoine MahulGaelle SamsonBrigitte ChebanceStéphanie BornesJérémie DenonfouxJérémie DenonfouxEric PeyretailladeMichael KatinkaMichael KatinkaValérie Polonaissubject
Transcription Geneticgenome annotationMESH : Molecular Sequence AnnotationGeneral Physics and AstronomyMESH: PhosphotransferasesGenometranscriptional signalMESH : Protein TransportMESH : Fungal ProteinsDNA FungalConserved SequenceComputingMilieux_MISCELLANEOUSGenetics0303 health sciencesFungal proteinMESH: Conserved SequenceMultidisciplinaryMESH: Genomics030302 biochemistry & molecular biologyGenomicsGenome projectProtein TransportMolecular Sequence Annotation[ SDV.BBM.GTP ] Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN]MESH: Genome FungalMESH: Fungal ProteinsMESH : PhosphotransferasesGenome FungalTransposable elementMESH: Protein TransportGenes FungalGenomicsMESH: Molecular Sequence AnnotationMESH : MicrosporidiaMESH : Open Reading FramesComputational biologyBiologyGeneral Biochemistry Genetics and Molecular BiologyFungal ProteinsOpen Reading Frames03 medical and health sciencesMESH : Conserved Sequence[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN]Anncaliia algeraeparasitic diseasesGene030304 developmental biologybioinformaticMESH: Transcription GeneticMESH : Genome FungalPhosphotransferasesstructural annotationMESH : GenomicsfungiMESH : Transcription GeneticMolecular Sequence AnnotationGeneral ChemistryMESH: Open Reading FramesMESH: MicrosporidiaMESH: DNA FungalmicrosporidiaMESH : Genes Fungal[INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM]MESH : DNA FungalMESH: Genes Fungaldescription
EA GenoSol CT3; International audience; High-quality annotation of microsporidian genomes is essential for understanding the biological processes that govern the development of these parasites. Here we present an improved structural annotation method using transcriptional DNA signals. We apply this method to re-annotate four previously annotated genomes, which allow us to detect annotation errors and identify a significant number of unpredicted genes. We then annotate the newly sequenced genome of Anncaliia algerae. A comparative genomic analysis of A. algerae permits the identification of not only microsporidian core genes, but also potentially highly expressed genes encoding membrane-associated proteins, which represent good candidates involved in the spore architecture, the invasion process and the microsporidian-host relationships. Furthermore, we find that the ten-fold variation in microsporidian genome sizes is not due to gene number, size or complexity, but instead stems from the presence of transposable elements. Such elements, along with kinase regulatory pathways and specific transporters, appear to be key factors in microsporidian adaptive processes.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2012-01-01 | Nature Communications |