6533b821fe1ef96bd127b7a9

RESEARCH PRODUCT

Influence of metformin on GLUT1 gene and protein expression in rat streptozotocindiabetes mellitusmodel

Olga SugokaLasma LauberteEvita RostokaSergejs IsajevsTatjana SjaksteNikolajs SjaksteDarja SvirinaJelena SharipovaJelizaveta SokolovskaIvars Kalvinsh

subject

Maleendocrine systemmedicine.medical_specialtyendocrine system diseasesPhysiologyCarbohydrate metabolismDiabetes Mellitus ExperimentalPhysiology (medical)Internal medicineDiabetes mellitusmedicineAnimalsHypoglycemic AgentsRats WistarGlycated HemoglobinGlucose Transporter Type 1Glucose tolerance testmedicine.diagnostic_testbiologybusiness.industrydigestive oral and skin physiologyGlucose transporternutritional and metabolic diseasesAMPKGeneral MedicineGlucose Tolerance TestStreptozotocinmedicine.diseaseMetforminRatsMetforminDisease Models AnimalGlucoseEndocrinologyGene Expression Regulationbiology.proteinGLUT1businessmedicine.drug

description

Metformin improves hyperglycaemia via mechanisms which include activation of AMP-activated protein kinase (AMPK). Recent findings indicate that some metabolic actions of metformin occur also by AMPK-independent mechanisms.To study the action of metformin on expression of GLUT1 glucose transporter in rat streptozotocin model of diabetes mellitus.Streptozotocin-induced rats were treated with metformin while monitoring parameters of carbohydrate and lipid metabolism. GLUT1 mRNA and protein expression in kidneys, heart, liver and muscles were studied by means of real time quantitative RT-PCR and immunohistochemistry correspondingly.Metformin treatment decreased glucose concentration, glycated haemoglobin % and improved glucose tolerance. Streptozotocin diabetes provoked increase of both GLUT1 gene and protein expression in kidneys, metformin treatment produced normalization of the GLUT1 expression levels. In the liver, diabetes triggered an increase in GLUT1 protein expression, which was normalized by metformin.Metformin is prospective for treatment of diabetic nephropathy.

yearjournalcountryeditionlanguage
2010-06-29Archives of Physiology and Biochemistry
https://doi.org/10.3109/13813455.2010.494672