6533b824fe1ef96bd127ffca
RESEARCH PRODUCT
1H and 13C HR-MAS spectroscopy of intact biopsy samples ex vivo and in vivo 1H MRS study of human high grade gliomas.
Bernardo CeldaOlivier AssematPilar FerrerAntonio RevertM. Carmen Martínez-bisbalLuis Martí-bonmatíMartial PiottoJosé L. LlácerJ. Piquersubject
AdultMaleMagnetic Resonance SpectroscopyResolution (mass spectrometry)Statistics as TopicNuclear magnetic resonanceIn vivoBiopsyMagic angle spinningmedicineHumansRadiology Nuclear Medicine and imagingSpectroscopyNuclear Magnetic Resonance BiomolecularSpectroscopyAgedCarbon Isotopesmedicine.diagnostic_testChemistryBrain NeoplasmsMiddle AgedIn vitroNeoplasm ProteinsMolecular MedicineFemaleProtonsGlioblastomaHeteronuclear single quantum coherence spectroscopyEx vivoBiomarkersdescription
High-resolution magic angle spinning (HR-MAS) one- and two-dimensional 1H and 13C nuclear magnetic resonance (NMR) spectroscopy has been used to study intact glioblastoma (GBM) brain tumour tissue. The results were compared with in vitro chemical extract and in vivo spectra. The resolution of 1H one-dimensional, 1H TOCSY and 13C HSQC HR-MAS spectra is comparable to that obtained on perchloric extracts. 13C HSQC HR-MAS spectra have been particularly useful for the identification of 37 different metabolites in intact biopsy tumours, excluding water and DSS components. To our knowledge, this is the most detailed assignment of biochemical compounds obtained in intact human tissue, in particular in brain tumour tissue. Tissue degradation during the recording of the NMR experiment was avoided by keeping the sample at a temperature of 4 degrees C. Detailed metabolical compositions of 10 GBM (six primary, two secondary and two unclassified) were obtained. A good correlation between ex vivo and in vivo MRS has been found.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2004-06-01 | NMR in biomedicine |