6533b824fe1ef96bd12812d9

RESEARCH PRODUCT

Determination of ascorbic acid in asparagus by differential pulse polarography

A. FrígolaRosaura FarréMaría J. Esteve

subject

Detection limitPolarographyChromatographybiologyPulse (signal processing)ChemistryExtraction (chemistry)biology.organism_classificationAscorbic acidBiochemistryAnalytical ChemistryVegetable ProductAsparagusQuantitative analysis (chemistry)

description

A useful method for the determination of ascorbic acid in a vegetable product (asparagus) by differential pulse polarography has been set up and evaluated. Extraction and instrumental conditions were optimized. The analytical parameters are: linearity (0–18.18 μg/ml); detection limit (0.182 μg/ml); instrumental and method precision (2.77% and 4%, respectively); accuracy (96.9–113.4%). These data show that the method is sufficiently sensitive, reliable and accurate. It was also compared with the official fluorometric AOAC method.

https://doi.org/10.1007/bf00323643