6533b829fe1ef96bd1289acb
RESEARCH PRODUCT
Generalised H-point standard addition method for the isolation of the analyte signal from the sample signal when coelution of unknown compounds occurs in liquid chromatography.
F Blasco GómezC. Molins LeguaP. Campíns FalcóF. Bosch ReigRosa Herráez-hernándezsubject
AnalyteInternal standardChromatographyChemistryOrganic ChemistryAmphetaminesAnalytical chemistryGeneral MedicineBiochemistrySample (graphics)SignalHigh-performance liquid chromatographyAnalytical ChemistryInterference (communication)PhenolsStandard additionSpectrophotometry UltravioletDiureticsQuantitative analysis (chemistry)Chromatography Liquiddescription
The generalised H-point standard addition method (GHPSAM) is proposed for isolating the analytical signal of an analyte from the signal of an unknown sample. Samples containing two and three coeluting compounds have been analysed. The accuracy of the predictions depends on the shape of the analyte and interferent spectra but not on the degree of chromatographic overlap. This methodology involves the location of linear intervals for the unknown interference spectrum from the spectrum of the sample. Once the linear interval has been found the selection of three wavelengths within the interval will allow the cancellation of the signal of the unknown interferent. The method has been applied to the determination of diuretics, amphetamines and phenols in water.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1999-09-11 | Journal of chromatography. A |