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Abstract This article presents a pulsed narrowband injection-locked Titanium:Sapphire laser aimed for high-resolution in-jet resonance ionization spectroscopy at the SLOWRI/PALIS at RIKEN. The laser has been integrated into the PALIS laser laboratory enabling it to be utilized with the existing broadband Titanium:Sapphire and dye lasers. The seed efficiency has been evaluated to be close to unity over the master laser wavelength range ∼ 753 to 791 nm, and the slope efficiency, namely the ratio of the pump power to the output power, was determined to be ∼ 30 % at 780 nm. A two-step ionization scheme with 386.4016 nm first step and 286.731 nm second step into an autoionizing state was developed for resonance ionization spectroscopy of 93 Nb . Magnetic hyperfine coupling constants of 1866 ± 8  MHz and 1536 ± 7  MHz were measured for the ground and excited state, respectively, in a good agreement with the literature values. A Gaussian dominated Voigt linewidth of 434.5 ± 7.4 MHz was extracted from the hyperfine spectra measured for niobium. In addition, the resolution of the in-jet resonance ionization in PALIS is estimated through numerical methods.