6533b829fe1ef96bd128afb6
RESEARCH PRODUCT
Modulation of protein synthesis and degradation maintains proteostasis during yeast growth at different temperatures
Paula AlepuzVicente TorderaJordi PlanellsAna MiguelTianlu LiFany CarrascoJosé E. Pérez-ortínMarta Benetsubject
0301 basic medicineSaccharomyces cerevisiae ProteinsTranscription GeneticRNA StabilitySaccharomyces cerevisiaeBiophysicsSaccharomyces cerevisiaeProtein degradationBiochemistryRibosomeRibostasis03 medical and health sciencesStructural BiologyGene Expression Regulation FungalGene expressionProtein stabilityGeneticsProtein biosynthesisHomeostasisRNA MessengerMolecular BiologyRegulation of gene expressionTranslation ratebiologyTemperaturebiology.organism_classificationYeastYeastCell biology030104 developmental biologyProteostasisBiochemistryProtein BiosynthesisProteostasisRibosomesdescription
To understand how cells regulate each step in the flow of gene expression is one of the most fundamental goals in molecular biology. In this work, we have investigated several protein turnover-related steps in the context of gene expression regulation in response to changes in external temperature in model yeast Saccharomyces cerevisiae. We have found that the regulation of protein homeostasis is stricter than mRNA homeostasis. Although global translation and protein degradation rates are found to increase with temperature, the increase of the catalytic activity of ribosomes is higher than the global translation rate suggesting that yeast cells adapt the amount of translational machinery to the constraints imposed by kinetics in order to minimize energy costs. Even though the transcriptional machinery is subjected to the same constraints, we observed interesting differences between transcription and translation, which may be related to the different energy costs of the two processes as well as the differential functions of mRNAs and proteins.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2016-12-29 |